Authors: Wasilewska et al.

Journal: International Journal of Biological Macromolecules (2023)

Institution: Polish Academy of Sciences

Research Areas: Materials Science

Cell Lines: MC3T3-E1 (Osteoblastic cell line)

Summary: The regulation of cellular adhesion is a crucial aspect in the development of biomaterials and cell-based biosensing assays. In this study, synthetic poly(diallyldimethylammonium chloride) (PDADMAC), natural chitosan, and heparin were utilized to assemble PDADMAC/heparin and chitosan/heparin films. The physicochemical properties of these macroion multilayers were characterized using streaming potential measurements (SPM), quartz crystal microbalance (QCM-D), and optical waveguide lightmode spectroscopy (OWLS), while their topography was imaged using atomic force microscopy (AFM). The adhesion of the preosteoblastic cell line MC3T3-E1 on these well-characterized polysaccharide-based multilayers was evaluated using a resonant waveguide grating (RWG) based optical biosensor and digital holographic microscopy HoloMonitor M4. Results showed that PDADMAC/heparin films were the most effective in inducing cellular adhesion, while chitosan/heparin-based multilayers exhibited negligible cell attachment. These findings suggest that polysaccharide-based multilayers have potential for use in medical applications.HoloMonitor M4 is used to study the cell proliferation and morphological parameters of preosteoblastic cells on different coatings.

Keywords: HoloMonitor M4, Cell proliferation, Cell morphology, Macroion multilayers, Polysaccharide layers, Label-free biosensors, Streaming potential, OWLS, Resonant waveguide grating, Cell adhesion, Antimicrobial coatings, QCM