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November 21, 2017

Cell Proliferation Assay

The HoloMonitor® Cell Proliferation Assay provides label-free growth curves by automatically monitoring cell count and confluence over time. Understand the growth pattern of your cells using the cell proliferation assay.

Automatic Presentation of Kinetic Cell Proliferation

Cell proliferation assays are widely applied in life science to understand the growth pattern of cultured cells and to assess the in vitro safety and efficacy of drugs over time. Traditional methods are end-point assays that often assess cell proliferation indirectly or are based on cell confluence measurements only. HoloMonitor® offers a convenient assay that automatically presents label-free cell proliferation data.

Cell Proliferation Assay Results

The cell count and confluence growths curve are updated and displayed throughout the experiment.

Excel Export of Cell Proliferation Data

After the experiment, cell proliferation data is easily exported to Excel (green arrow in the above image).

Cell Proliferation using HoloMonitor® – Kinetic Cell Count and Confluence Assessment

HoloMonitor offers a robust and label-free kinetic cell proliferation assay with high temporal resolution based on direct and continuous cell counting:

  • Kinetic assessment of both cell count and cell confluence
  • Individual cells are automatically identified and counted by the system software
  • The analysis includes data for both individual cells and entire populations at the same time.

Experiments setup using App Suite software can eaily be re-analysed to generate results for another assay type. For example, for a proliferation experiment, motility results can be achieved from the same experimental data.

HoloMonitor video of proliferating cells, showing frequent cell divisions.

Key References Cell Proliferation

  • General Cytotoxicity and Its Application in Nanomaterial Analysis
    M. Jedrzejczak-Silicka and E. Mijowska
    IntechOpen (2018)

    Review describing and comparing various assays used to study biocompatibility and cytotoxicity of nanomaterials. Holographic phase imaging is pointed out as an excellent tool for cell morphometric characterisation and cell migration studies, and the authors conclude that the interest in the use of DH microscopy in research is constantly increasing.

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  • Label-free High Temporal Resolution Assessment of Cell Proliferation Using Digital Holographic Microscopy
    Birgit Janicke, Andreas Kårsnäs, Peter Egelberg and Kersti Alm
    Cytometry Part A (2017)

    The authors have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using HoloMonitor M4. Only two image processing settings were adjusted between cell lines, making the assay practical, user friendly, and free of user bias. In the recorded time-lapse image sequences, individual cells were automatically identified to provide detailed growth curves and growth rate data of cell number, confluence, and average cell volume. The results demonstrate how these parameters facilitate a deeper understanding of cell processes than what is achievable with current single-parameter and end-point methods.

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