Cell Culture Quality Control
Label-free, non-invasive cell culture quality control (cell QC) of your cells — inside the cell incubator.
It is well known that when cells are cultured over longer or shorter time periods they may undergo morphological changes. Quick assessment of the health and quality of a cell culture prior to experiments may, therefore, be useful to avoid unexpected or inferior results.
Since no labels or stains are needed when running a HoloMonitor assay, experimental setup is quick and easy. However, to ensure optimal experimental conditions, a quick cell quality control is recommended prior to imaging.
Cell QC — Ensures Cell Health and Optimal Setup
The HoloMonitor® Cell Quality Control assay can be used to ensure that the cell count and/or confluence is appropriate at start of an experiment. It can also be used as a tool to detect undesired changes in your cell culture as compared to previous experiment(s). We recommend that the QC is performed regularly on cells growing in log phase.
Detects morphological changes
The assay automatically presents cell count and confluence, as well as basic morphology of adherent cells.
Distribution graphs can help to detect any undesired changes in the cell culture.
Non-invasive, Quantitative Assessment of the Morphology of y-irradiated Human Mesenchymal Stem Cells and Periosteal Cells Using Digital Holographic MicroscopyInternational journal of radiation biology (2016)Read more
Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.
Non-invasive, Label-free Cell Counting and Quantitative Analysis of Adherent Cells Using Digital HolographyJournal of Microscopy (2008)Read more
Using a unique non-invasive label-free cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HoloMonitor™ M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.