Cell Culture Quality Control
Avoid contaminated cells. Ensure the quality of your cells with a cell culture quality control (cell QC) assay, and detect undesired cell changes. HoloMonitor cell quality control assay provides a label-free solution to validate cell health inside your incubator.
The importance of cell quality control
It is well known that when cells are cultured over longer or shorter time periods they may undergo morphological changes. Quick assessment of the health and quality of a cell culture prior to experiments may, therefore, be useful to avoid unexpected or inferior results.
Since no labels or stains are needed when running a HoloMonitor assay, experimental setup is quick and easy. However, to ensure optimal experimental conditions, a quick cell quality control is recommended prior to imaging.
Cell QC — Ensures Cell Health and Optimal Setup
The HoloMonitor® Cell Quality Control assay can be used to ensure that the cell count and/or confluence is appropriate at start of an experiment. The cell quality control assay can also be used as a tool to detect undesired changes in your cell culture as compared to previous experiment(s). We recommend that the cell QC is performed regularly on cells growing in log phase.
Detects cell morphological changes
The assay automatically presents cell count and confluence, as well as basic morphology of adherent cells.
Distribution graphs can help to detect any undesired changes in the cell culture.
Key Cell Quality Publications
Non-invasive, Label-free Cell Counting and Quantitative Analysis of Adherent Cells Using Digital HolographyJournal of Microscopy (2008)Read the article
Using a unique non-invasive label-free cell counting method comparable to conventional cell counting using a hemocytometer was produced. The major advantage of using HoloMonitor™ M2 is the opportunity to easily access information about cell number, size, optical thickness, and confluence in an automatic, non-invasive manner.
Non-invasive, Quantitative Assessment of the Morphology of y-irradiated Human Mesenchymal Stem Cells and Periosteal Cells Using Digital Holographic MicroscopyInternational journal of radiation biology (2016)Read the article
Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.