Cell Culture Quality Control

Label-free, non-invasive cell culture quality control (cell QC) of your cells — inside the cell incubator.

It is well known that when cells are cultured over longer or shorter time periods they may undergo morphological changes. Quick assessment of the health and quality of a cell culture prior to experiments may, therefore, be useful to avoid unexpected or inferior results.

Since no labels or stains are needed when running a HoloMonitor assay, experimental setup is quick and easy. However, to ensure optimal experimental conditions, a quick cell quality control is recommended prior to imaging.

Cell QC — Ensures Cell Health and Optimal Setup

The HoloMonitor® Cell Quality Control assay can be used to ensure that the cell count and/or confluence is appropriate at start of an experiment. It can also be used as a tool to detect undesired changes in your cell culture as compared to previous experiment(s). We recommend that the QC is performed regularly on cells growing in log phase.

Detects morphological changes

The assay automatically presents cell count and confluence, as well as basic morphology of adherent cells.

Distribution graphs can help to detect any undesired changes in the cell culture.

Instant Cell Quality Assurance using HoloMonitor®

The HoloMonitor Cell QC assay reveals end-point data on cell population level. It is designed to be used as a tool to detect undesired changes in your cell culture as compared to previous experiments. As all HoloMonitor applications, the assay is cell-friendly and based on label-free live-cell imaging.

Endpoint data are given as values per well/condition:

  • Cell count (cells/cm2)
  • Mean cell area (µm2)
  • Mean cell diameter (µm)
  • Mean cell volume (µm3)
  • Confluence (%)

If needed, further analyses can be undertaken for details on cell morphology and other parameters.

Key Publications


  • Non-invasive, Quantitative Assessment of the Morphology of y-irradiated Human Mesenchymal Stem Cells and Periosteal Cells Using Digital Holographic Microscopy
    Tomoyuki Kawase, Kazuhiro Okuda, Masaki Nagata, Makoto Tsuchimochi, Hiromasa Yoshie & Koh Nakata
    International journal of radiation biology (2016)

    Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.

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  • Non-invasive, Label-free Cell Counting and Quantitative Analysis of Adherent Cells Using Digital Holography
    Anna Mölder et al
    Journal of Microscopy (2008)

    Using a unique non-invasive label-free cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HoloMonitor™ M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.

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