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It is well known that when cells are cultured over longer or shorter time periods they may undergo morphological changes. Quick assessment of the health and quality of a cell culture prior to experiments may therefore be useful to avoid unexpected or inferior results.
Since no labels or stains are needed when running a HoloMonitor assay, experimental setup is quick and easy. However, to ensure optimal experimental conditions, a quick cell quality control is recommended prior to imaging.
HoloMonitor® Cell Quality Control assay can be used to ensure that the cell count and/or confluence is appropriate at start of an experiment. It can also be used as a tool to detect undesired changes in your cell culture as compared to previous experiment(s). We recommend that the QC is performed regularly on cells growing in log phase.
The assay automatically presents cell count and confluence, as well as basic morphology of adherent cells.
Distribution graphs can help to detect any undesired changes in the cell cuture.