A HoloMonitor microscope placed on a white lab bench with cell lab tools.

HoloMonitor® — A Cell Culture Microscope

The inverted HoloMonitor cell culture microscope gives you label-free, single-cell analysis on a population level. It works in real-time within a CO₂ incubator or hypoxia chamber. This lets you do long-term studies of in vitro cell cultures in a stable and physio­logi­cally relevant environment. HoloMonitor non-invasively quantifies more than 30 cellular parameters reflecting cell proliferation, cell migration and invasion, and cell morphology and differentiation.

The inverted HoloMonitor live cell imaging microscope system provides more than 30 cell parameters, reflecting cell culture health, proliferation, migration, differentiation and cell cycle phase.

Cell-friendly, long-term analysis

The HoloMonitor cell culture microscope is an incubator tolerant cell culture imager. This makes it suitable for long-term live cell analysis. Better yet, using quantitative phase-contrast microscopy, it images your cells with no labels or stains. When you place it in a CO₂ incubator, you can monitor your cell cultures under optimal conditions. Hence, HoloMonitor allows cell analysis for long periods of time, without having the imaging procedure or instrument affect your cells.

HoloMonitor cell morphology assay: cell morphology analysis using gated scatter plot

Single-cell analysis on a population level

HoloMonitor is a cell culture microscope, which means it images and analyzes cell cultures. However, each image it takes contains data on more than 30 cellular parameters on every single cell. Therefore, you can analyze your cell cultures not only at a population level but down to the single-cell level. The time-lapse images are automatically analyzed as your experiment goes on. Hence, it gives you graphs and scatterplots on single-cell and population data in real-time.

Workflow of the HoloMonitor live cell imaging system

Reuse your cells after the experiment

HoloMonitor does not need labeling, stained or modified cells to be able to image them. The low-energy laser keeps phototoxicity to a minimum, and it images the cells in their normal environment. This means your cells are completely untouched after your HoloMonitor experiment is finished. Therefore, you can use your cells for further studies using other instru­mentation, for example, fluorescent imaging by high-resolution confocal microscopy.

The HoloMonitor M4 is a beneficial device for live-cell imaging, providing a lot of data. The device does not require large space and the software application is user-friendly, while recorded pictures look great. The good thing is that you can analyze your experiment using one software assay, and later, you can rerun the recorded data in another assay, which allows you to get the maximum from your experiment.

Sasa Vasilijic, PhD
Stanford University

Cell culture analysis with standard cell culture vessels

Pipetting with Hololid in 6-well microplate imaging inside incubator microscope

Cell culture microscopes made for live cell imaging often require specially designed cell culture vessels. These vessels are usually expensive and are tailor-made for a specific purpose. On the contrary, the HoloMonitor cell culture microscope was designed to use standard cell culture vessels, such as 35 mm Petri dishes, 96-well plates or microslides. Hence, you can prepare your experiments in your standard vessels. This saves not only money but also streamlines your daily cell culture work by reducing the amount of specially designed consumables. HoloMonitor adapts to you, not the other way around.