The PHI solution for studying dose-response relationships enables you to measure dose-response curves of drugs over time. By letting you continuously image and study your cells directly inside your incubator, you will generate more accurate results and save time:
Created from a single in vitro experiment, the HoloMonitor dose-response curves are interactive and displayed for the time-point selected using the timeline.
The above dose-response result was created by recording a time-lapse movie of each well for 48 hours.
Modeling the desired effect of a new drug candidate in vitro and understanding the dose-response relationship of drugs over time is essential when developing effective treatment strategies in cancer research or pharmacology.
This makes the dose-response curve of adherent cells a vital tool in drug screening and pre-clinical research, for example in determining threshold dose, pharmacological profiles and site of action.
The dose-response relationship allows drug dose-response and potency to be determined in vitro and provide guidance for future studies. Additionally, knowledge of drug effects on cell population morphology facilitates advances in drug development and future disease treatments.
Kendra L. Barker et al., Label-Free Classification of Apoptosis, Ferroptosis and Necroptosis Using Digital Holographic Cytometry, Applied Sciences (2020)
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A quick video demonstrating the HoloMonitor App Suite software.
In addition to the dose-response relationship, associated changes in basic morphology are given. All data is easily exported to Excel for further analysis.
Screenshot of the HoloMonitor Dose-Response Assay — showing cell count, confluence and mean cell volume over time.
HoloMonitor provides a detailed analysis of drug effects and toxicity at selected time points:
When preferred, further analyses can be undertaken for details on cell morphology changes, cell proliferation and cell death kinetics using additional HoloMonitor assays.