March 12, 2019

Conference Papers and Posters

  • Controlled Conditions Reduce Critical Edge Effect in 96-Well Plates
    Allayna M. Frank, Stassa Henn, Shannon Darou, Alicia D. Henn, Randy Yerden
    SOT - Society of Toxicology (2019)

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  • Eliminating Edge Effect in 96-well Plates by Controlling Thermal Conditions during Cell Plating
    Shannon Darou1 , Alicia D. Henn1 , Allayna M. Frank1 , Kersti Alm2 , Randy Yerden
    AACR - American Association for Cancer research annual meeting (2019)

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  • Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holo­graphic imaging
    Ed Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. Torchilin
    AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)

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  • Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture Models
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    AACR Annual Meeting (2017)
    4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
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  • Cystatin C Decreases Proliferation of Melanoma Cells by Affecting Mitosis Length
    Hanna Wallin, Kersti Alm and Magnus Abrahamson
    AACR Annual Meeting (2017)
    Analysis using HoloMonitor revealed qualitative differences in the normal behavior of the A375 cells under study as a result of cystatin C addition. The motility of the cells was significantly affected. The mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the mitosis phase.
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  • HoloMonitor M4: holographic imaging cytometer for real-time kinetic label-free live-cell analysis of adherent cells
    Mikael Sebesta; Peter J. Egelberg; Anders Langberg; Jens-Henrik Lindskov; Kersti Alm; Birgit Janicke
    Proceedings, Quantitative Phase Imaging II (2016)

    Live-cell imaging enables studying dynamic cellular processes that cannot be visualized in fixed-cell assays. An increasing number of scientists in academia and the pharmaceutical industry are choosing live-cell analysis over or in addition to traditional fixed-cell assays. We have developed a time-lapse label-free imaging cytometer HoloMonitor M4. HoloMonitor M4 assists researchers to overcome inherent disadvantages of fluorescent analysis, specifically effects of chemical labels or genetic modifications which can alter cellular behavior. Additionally, label-free analysis is simple and eliminates the costs associated with staining procedures.

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  • Transcription Regulation of Chemokine Receptor CXCR4 by Nuclear Respiratory Factor 1 (NRF1) Controls Estrogen-Induced Malignant Transformation of Breast Epithelial Cells to Breast Cancer Stem Cells
    J. K. Das and D. Roy
    AACR Annual Meeting (2016)
    Chemokine receptor CXCR4 is involved in the maintenance of stemness property of stem cells, aging and metastasis of cancer. NRF1 is one of the major transcription factors that controls transcription of CXCR4. In this study, the authors have investigated whether transcription regulation of CXCR4 by NRF1 controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells.
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  • A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov Test
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    3rd Conference on Impedance-Based Cellular Assays (2016)

    Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.

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  • Impedance-Based Analysis and Holographic Phase Imaging of The GNRH-Iii-Based Drug-Targeting In Melanoma Cells
    E. Lajkó, S. Spring, O. Láng, S. Ingebrandt, G. Mező and L. Kőhidai
    3rd Conference on Impedance-Based Cellular Assays (2016)

    HoloMonitor M4 was used for the morphometry and migration studies.

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  • Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imaging
    Ed Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang, Mansoor Amiji.
    AACR Annual Meeting (2015)

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