Conference Papers and Posters
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Controlled Conditions Reduce Critical Edge Effect in 96-Well PlatesAllayna M. Frank, Stassa Henn, Shannon Darou, Alicia D. Henn, Randy YerdenSOT - Society of Toxicology (2019)
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Eliminating Edge Effect in 96-well Plates by Controlling Thermal Conditions during Cell PlatingShannon Darou1 , Alicia D. Henn1 , Allayna M. Frank1 , Kersti Alm2 , Randy YerdenAACR - American Association for Cancer research annual meeting (2019)
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Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holographic imagingEd Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. TorchilinAACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)
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Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture ModelsEd Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir TorchilinAACR Annual Meeting (2017)4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
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Cystatin C Decreases Proliferation of Melanoma Cells by Affecting Mitosis LengthHanna Wallin, Kersti Alm and Magnus AbrahamsonAACR Annual Meeting (2017)Analysis using HoloMonitor revealed qualitative differences in the normal behavior of the A375 cells under study as a result of cystatin C addition. The motility of the cells was significantly affected. The mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the mitosis phase.
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HoloMonitor M4: holographic imaging cytometer for real-time kinetic label-free live-cell analysis of adherent cellsMikael Sebesta; Peter J. Egelberg; Anders Langberg; Jens-Henrik Lindskov; Kersti Alm; Birgit JanickeProceedings, Quantitative Phase Imaging II (2016)Read more
Live-cell imaging enables studying dynamic cellular processes that cannot be visualized in fixed-cell assays. An increasing number of scientists in academia and the pharmaceutical industry are choosing live-cell analysis over or in addition to traditional fixed-cell assays. We have developed a time-lapse label-free imaging cytometer HoloMonitor M4. HoloMonitor M4 assists researchers to overcome inherent disadvantages of fluorescent analysis, specifically effects of chemical labels or genetic modifications which can alter cellular behavior. Additionally, label-free analysis is simple and eliminates the costs associated with staining procedures.
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Transcription Regulation of Chemokine Receptor CXCR4 by Nuclear Respiratory Factor 1 (NRF1) Controls Estrogen-Induced Malignant Transformation of Breast Epithelial Cells to Breast Cancer Stem CellsJ. K. Das and D. RoyAACR Annual Meeting (2016)Chemokine receptor CXCR4 is involved in the maintenance of stemness property of stem cells, aging and metastasis of cancer. NRF1 is one of the major transcription factors that controls transcription of CXCR4. In this study, the authors have investigated whether transcription regulation of CXCR4 by NRF1 controls estrogen-induced malignant transformation of breast epithelial cells to breast cancer stem cells.
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A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov TestEd Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin3rd Conference on Impedance-Based Cellular Assays (2016)
Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.
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Impedance-Based Analysis and Holographic Phase Imaging of The GNRH-Iii-Based Drug-Targeting In Melanoma CellsE. Lajkó, S. Spring, O. Láng, S. Ingebrandt, G. Mező and L. Kőhidai3rd Conference on Impedance-Based Cellular Assays (2016)
HoloMonitor M4 was used for the morphometry and migration studies.
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Characterization of macrophage behavior by 4-dimensional label free quantitative holographic imagingEd Luther, Meghna Talekar, Adwait Oka, Qijun Ouyang, Mansoor Amiji.AACR Annual Meeting (2015)