Northeastern University – Holographic Imaging Cytometry Program of Excellence

The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis. The program covers several activities including:

  • Establishing a network of collaborators utilizing Holographic Imaging Cytometry.
  • Developing novel applications supporting research interests of the Northeastern faculty and students.
  • Presenting results at scientific meetings.
  • Organizing an annual technology symposium and hands-on laboratory courses.

Achievements

In May 2017, a summary of the scientific achievements was published in a special issue of Cytometry part A, dedicated to holographic microscopy and quantitative phase imaging.

Read the summary here and view the associated CYTO 2017 presentation here.

Poster session at AACR Annual Meeting 2017. View poster presented by Ed Luther, Supervisor Core Imaging and Cytometry Facility at Northeastern University here.

The expansion of our existing collaboration with PHI is the result of productive utilization of HoloMonitor technology for non-invasive real-time cell cycle analysis in living cells. We are now developing state-of-the art applications multiplexing cell cycle and cell motility.

Ed Luther

Northeastern University

Peer Reviewed Articles and Book Chapters

  • Applications of quantitative time lapse holographic imaging to the development of complex pharmaceutical nano formulations
    E Luther, L Mendes, J Pan, D Costa, C Sarasozen and V Torchilin
    SPIE BiOS Conference Proceedings (2018)

    We rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. We employ an incubator-adapted, label-free holographic imaging cytometer HoloMonitor M4® (Phase Holographic Imaging, Lund, Sweden) to obtain multi-day time-lapse sequences at 5- minute intervals. An automated stage allows hand-free acquisition of multiple fields of view.

    Read more
  • Applications of Label-free, Quantitative Phase Holographic Imaging Cytometry to the Development of Multi-specific Nanoscale Pharmaceutical Formulations
    Luther E et al
    Cytometry Part A 2017 (2017)

    A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2

    Read more
  • Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of a p-glycoprotein Inhibitor and Paclitaxel in a Liposomal Platform
    Y. Zhang et al.
    Molecular Cancer Therapeutics (2016)

    The authors created a nanoparticle formulation against chemotherapy resistant ovarian cancer, loaded with the commonly used cancer drug paclitaxel together with a drug that breaks down the resistance mechanism of cancer cells. Using HoloMonitor, the scientists were able to show that paclitaxel-resistant ovarian cancer cells stopped multiplying when treated with the nanoparticles.

    Read more

Conference Papers and Posters

  • Comparison of the effects of pharmaceutical compounds on tumor cells in 2D and 3D in vitro models using label-free, quantitative 4 dimensional holo­graphic imaging
    Ed Luther, Giuseppina Salzano, Shravan Kumar Sriraman, Daniel Costa and Vladimir P. Torchilin
    AACR-NCI-EORTC Inter. Conf. on Molecular Targets and Cancer Therapeutics 2015 (2018)
  • Development of a 4-Sample Version of the Kolmogorov-Smirnov Test for Evaluating the Temporal Physiology of Cells Treated with test Compounds in a Label-Free, High Content, Platform for Quantitative Analysis of Adherent Cell-Culture Models
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    AACR Annual Meeting (2017)
    4-dimensional plots of HoloMonitor time-lapse data were developed to assist in the visualization of the effects of pharmaceutical compounds under development.
  • A Label-Free, High Content, Moderate Throughput Analytical Platform for Quantitative Kinetic Analysis of Cell Behavior upon Drug Activation Based on the Kolmogorov-Smirnov Test
    Ed Luther, Livia Mendes, Jiayi Pan, Daniel Costa, Elena Holden and Vladimir Torchilin
    3rd Conference on Impedance-Based Cellular Assays (2016)
    “Our objective is to develop multi-functional nanotechnology-based antitumor drug delivery systems for improving efficacy of treatments and reducing undesirable side effects. The essential part of this process is the development of un-biased quantitative analytical techniques. We are reporting a successful validation of a very high content, medium throughput system in multi-well plates, employing the newly developed holographic imaging cytometer HoloMonitor® M4 for label-free time-lapse cellular analysis.”

    View poster

Featured Applications

Chemoresistance

Macrophage Polarization

Macrophage Response to Polarization

Location

Department of Pharmaceutical Sciences
School of Pharmacy
Bouvé College of Health Sciences
Northeastern University
140 The Fenway, Room 228/229
60 Huntington Avenue
Boston, Massachusetts 02115