Time-lapse Imaging Cytometry

Time-lapse imaging cytometry by holographic microscopy allows non-invasive visualization and analysis of live cell populations by tracking and quantifying individual cells.

Understanding Cellular Dynamics

An advantage of holographic microscopy is that the created quantitative phase images are focused when viewed, not when recorded. This makes the HoloMonitor time-lapse cytometer ideal for long-term imaging and analysis of living cells by means of time-lapse micro­scopy, which acquires a series of cell images at regular time intervals to analyze the dynamics of various cellular events. Un­focused images, caused by focus drift, are simply refocused by letting the computer soft­ware recreate the phase image from the recorded hologram.

A time-lapse microscopy image showing a Monster HeLa cell, acquired by the HoloMonitor imaging cytometer.

An example time-lapse image sequence of a monster HeLa cell, imaged by HoloMonitor. HeLa cells are cancer cells named after Henrietta Lacks, who in the early 1950s donated the first cells that was successfully kept alive and cultured in a laboratory environment. HeLa cells and other immortal cells are today routinely cultured by scientists to study the complex behavior of cells and their response to drug treatments.

In addition to identifying each individual cell, HoloMonitor provides data for analysis of more than 30 morphological parameters. However, the true power of time-lapse cytometry first emerges when the same cells are monitored over time. The HoloMonitor design utilizes recent tech­nological advances to allow time-lapse image sequences of cultured cells to be effortlessly recorded over long time periods.

With HoloMonitor installed in a cell incubator, the cells are kept in a cell friendly environment during the entire experiment. Long-term live cell kinetic data can easily be obtained using time-lapse microscopy. Images are recorded at selected intervals, down to 1 image/sec. Depending on the app­lication, cell images are played back as a video recording to aid analysis of dynamic cell behavior.

A wound healing time-lapse image sequence of a wound healing scratch assay, acquired by holographic time-lapse microscopy using the HoloMonitor imaging cytometer.

Time-lapse image sequence of a wound healing assay experiment.

Cytometric Imaging Software

From recorded time-lapse image sequences, the Holo­Monitor App Suite software helps the user to auto­matically extract and kinetically analyze live cell population data based on individual cell data. When preferred, individual cell data — such as cell count, cell morphology, cell velocity, and cell division rate — can all be measured from the same experimental time-lapse data, without requiring additional experiments.

HoloMonitor App Suite software

HoloMonitor App Suite

Time-lapse Cytometry References

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