HoloMonitor — A Quantitative Live Cell Imager

The HoloMonitor live cell imager facilitates long-term in vitro imaging of cell cultures within an incubator. Scientists across the world use HoloMonitor to non-invasively image and to auto­matically obtain cell morphology, proliferation and migration data on a single-cell level, in real-time.

A Cell-friendly Imager

The non-invasive core technology, holographic microscopy, is label-free, cell-friendly, fast and simple to use — offering unique imaging capabilities for long-term live cell kinetic investigations at single-cell and cell population level.

Developed by and for cell biologists, the HoloMonitor live cell imager is designed to in vitro visualize and quantify adherent cells in real-time in a CO2 cell incubator environment. The App Suite cell imaging and analysis software allows researchers to create publi­cation-ready images, videos and diagrams of cellular drug response and behavior, using a range of applications — all label-free.

An apoptotic cell explosion imaged by the HoloMonitor live-cell microscope.
Quantitative phase image of an apoptotic cell explosion, created by HoloMonitor.

Visualizes and Quantifies Cellular Events

Without requiring labels or stains, HoloMonitor generates both visual elements and quantitative data. Visual elements — such as sequences of cell images and time-lapse videos — illustrate cellular events taking place during the experiment, while the quantitative data puts numbers on what can be seen with the eye.

Single-cell Imaging & Analysis

The label-free quantitative phase images recorded by HoloMonitor allow the HoloMonitor software to automatically identify and quantify individual cells in standard cell culture samples. As the cell culture sample is being automatically imaged and analyzed, the individual cell measurements are processed to provide kinetic population data of cell morphology, proliferation and migration in real-time.

Additionally, the in-depth Cell Tracking Assay allows individual cells to be tracked over time to detect and quantify rare heterogeneous cellular events.

A Cell Imager for Incubators and Hypoxia Chambers

  • Cell-friendly

    HoloMonitor allow cell cultures to be continuously imaged and analyzed over extended periods of time under physiologically optimal conditions — in a standard CO2 incubator or a hypoxia chamber. As all HoloMonitor applications are completely label-free, the risk of toxic effects masking drug-induced effects is reduced to a minimum.
  • Reliable and Cost-effective Operation

    Since no labels or stains are needed, the assay protocols are straight forward and the experiments are quick and easy to set-up. A new and innovative mechanical design together with an intuitive software interface makes HoloMonitor operation simple and reliable.
  • Single-cell Analysis on a Population Level

    Images can be recorded with high temporal resolution, ensuring that important cellular events are not overlooked. This high temporal resolution is a key reason why HoloMonitor is optimal for kinetic live cell studies to advance the understanding of processes such as cell growth, movements, division and cell death.
  • Long-term Live Cell Experiments

    The incubator-compatible design makes HoloMonitor especially well suited for long-term kinetic cellular analysis. The low-intensity single-wavelength laser generates no heat, reducing the risk of photo-damage to an absolute minimum.
The HoloMonitor live cell imaging system operating inside a standard cell incubator at the SmiLe life science business incubator in Lund, Sweden.
HoloMonitor in operation inside a cell incubator at the SmiLe life science business incubator in Lund, Sweden.