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Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy

Cancer cells differ from other cells when it comes to cell morphology, cell proliferation, cell division, cell migration and cell motility. To investigate cell division, the researchers behind this article, used HoloMonitor to track cell movements. Holographic time-lapse movies were used to extract information on cell division of individual cells, and cell family trees were constructed where the cells were categorized.

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Date	Title	Journal	Author(s)	Country	Research area	Applications	Cell line(s)	Cell line(s) description	Technology	Conclusions
03/05/2021	Tuning Anti-Biofilm Activity of Manganese(II) Complexes: Linking Biological Effectiveness of Heteroaromatic Complexes of Alcohol, Aldehyde, Ketone, and Carboxylic Acid with Structural Effects and Redox Activity	International Journal of Molecular Sciences	Jabłońska-Wawrzycka et al.	Poland	Antibiotics	Cell counter	VH10	VH10 - Human foreskin fibroblasts		Antibiotic resistance is a rising issue worldwide and becoming one of the most challenging problems in our time. Thus alternative antimicrobial therapies are urgently required. In this study, the authors have used new manganese (II) complex to target bacterial pathogen by inhibiting the biofilm formation and other antibacterial activity in the primary human fibroblast (VH10) model. HoloMonitor M4 was used to count cell numbers after various treatments to study cell viability.
16/05/2021	Mast Cell Proteases Tryptase and Chymase Induce Migratory and Morphological Alterations in Bronchial Epithelial Cells	International Journal of Molecular Sciences	F. Berlin et al.	Sweden	Cell Research	Cell growth, cell division, cell morphology, cell migration, cell motility	BEAS-2B	BEAS-2B - Human bronchial epithelial cells	HoloMonitor M4	Mast cells (MCs) home into the epithelium in respiratory diseases, but the interaction mechanisms remain unclear. This study investigates how MC tryptase and chymase affect bronchial epithelial cells. HoloMonitor M4 was used to study cell growth and division rate, cell morphology changes, and cell motility and migration. The authors conclude that MC proteases induce cell migration and proliferative and morphological alterations in bronchial epithelial cells, suggesting that MC release of proteases may play a critical role in airway epithelial remodeling and disruption of epithelial function.
13/04/2021	The implication of ROCK 2 as a potential senotherapeutic target via the suppression of the harmful effects of the SASP: Do senescent cancer cells really engulf the other cells?	Cellular Signalling	Y. D. Simay et al.	Turkey	Cell Research	Cell morphology	HeLa and A549	HeLa - cervical cancer cell line; A549 - human lung adenocarcinoma cells	HoloMonitor M4	Senescence-associated secretory phenotype (SASP) is responsible for chemotherapy adverse effects: drug resistance and the induction of cancer cell proliferation, migration, and invasion. Hence, developing senolytic/semomorphic drugs, targeting senescence cells, are important to improve cancer treatment outcome. In this work, Y. D. Simay with colleagues evaluated if Rho/Rho kinase pathway could be a target for potential drugs in doxorubicin-induced senescent cancer cell lines. Authors have shown that ROCK inhibitors (i.e. ROCK2) have the potential to be developed into semomorphic drugs as they change SASP composition and reduce senescence cell secretory activity. HoloMonitor M4 was used to study cell morphology (cell area, optical volume, and optical thickness).
08/04/2021	Externally added cystatin C reduces growth of A375 melanoma cells by increasing cell cycle time	FEBS Openbio	H. Wallin et al.	Sweden	Cancer research	Cell proliferation, cell morphology, cell cycle	A375	A375 - human malignant melanoma	HoloMonitor M4	Human cell growth and intracellular proteolysis is affected by naturally secreted cysteine protease inhibitors, thus it could potentially be used to suppress tumour growth. In this paper, H. Wallin with colleagues have studied the effects of externally added Cystatin C on melanoma cancer cell viability and proliferation. Authors have shown that the addition of Cystatin C and its internalization prolongs cell cycle length (division) and decreases melanoma cell proliferation and could potentially be utilized in suppressing tumor growth. HoloMonitor M4 was used to study cell proliferation, cell cycle length, and cell morphology (area and optical thickness).
06/04/2021	Evaluation of the Toxicity on Lung Cells of By-Products Present in Naphthalene Secondary Organic Aerosols	Life	Y. L. de Albuquerque et al.	France	Aerosol toxicity	Cell morphology, cell movements,	A549	A549 - human lung adenocarcinoma cells	HoloMonitor M4	Due to pollution, around 7 million people died in 2018. One of the most toxic particles in the air is Phthalic acid (PA) and its derivatives. Thus, in this paper, Y. L. de Albuquerque with colleagues have studied the toxicity of PA by-products on lung cells. Authors have determined that 1,4-NQ is the most toxic by-product and highlights the complementary of RTCA and the HoloMonitor microscope for studying effects on lung cells induced by chemicals. HoloMonitor M4 was used to study A549 cell morphology (area, optical volume, perimeter) and movements (migration, motility, and motility speed) after exposure to various chemicals.
26/03/2021	Epac1 signaling pathway mediates the damage and apoptosis of inner ear hair cells after noise exposure in a rat model	Neuroscience	F. Sun et al.	China	Hearing loss studies	Cell proliferation	HEI-OC1	HEI-OC1 - inner ear cell line	HoloMonitor M4	Noise-induced hearing loss (NIHL) is a major type of sensorineural hearing loss. However, to date, there are no approved drugs for treatment. In this study, F. Sun with colleagues have investigated the role exchange proteins activated (Epac) by cAMP in inner ear hair cell damage and apoptosis after noise exposure. Authors have shown that Epac1 inhibitor protects from NIHL and makes it a potential therapeutic target. HoloMonitor M4 was used to study HEI-OC1 cell proliferation.
02/03/2021	Targeted Delivery of Combination Therapeutics Using Monoclonal Antibody 2C5-Modified Immunoliposomes for Cancer Therapy	Pharmaceutical Research	R. Narayanaswamy and V. P. Torchilin	USA	Drug activity	Cell Proliferation and Cell Morphology	MDA-MD-231	MDA-MB-231 - Human mammary carcinoma cell line;	HoloMonitor M4	Improving the various properties, such as biodistribution and solubility, are the main driving force for using nano-carries in drug deliveries. In this study, the authors have used monoclonal antibody modified immunoliposomes for targeted delivery of paclitaxel and salinomycin for cancer therapy. Researchers have shown that a combination of drugs increases therapeutical efficacy when delivered in immunoliposomes. HoloMonitor M4 was used to study cell killing, cell division and proliferation post-treatment.
23/02/2021	Cytoplasmic localization of prostate-specific membrane antigen inhibitors may confer advantages for targeted cancer therapies	Cancer Research	J. Matthias et al.	Germany	Cytotoxicity	Cell Proliferation	LNCaP and 22Rv1	LNCaP - androgen-sensitive human prostate adenocarcinoma cell line; 22Rv1 - prostate carcinoma epiithelial cell line	HoloMonitor M4	The metastatic, castration-resistant prostate cancer represents a major therapeutic challenge as treatment options are limited to only a few options, such as prostate-specific membrane antigen (PSMA) inhibitors. However, the detailed understanding of the mode of action inside the cells and the internalization mechanism of these molecules are still unknown. In this publication, J. Matthias with colleagues have synthesized a fluorescence-labeled PMSA inhibitors and elucidated their uptake mechanism and fate inside the cell using STED nanoscopy. HoloMonitor M4 was used to evaluate fluorescence molecule cytotoxicity and revealed that they do not affect cell proliferation.
09/02/2021	A novel ROCK inhibitor: off-target effects of metformin	Turkish Journal of Biology	A. Özdemir et al.	Turkey	Drug activity	Cell morphology	HeLa and MCF-7	HeLa - cervical cancer cell line; MCF-7 breast cancer cell line;	HoloMonitor M4	Drug discovery and approval is a lengthy process. Thus in recent years, already approved drugs are tested against nontarget treatments. In this study, A. Özdemir with colleagues have tested two small molecules, metformin and tolbutamide, as Rho kinase (ROCK) inhibitors. Authors have shown that metformin has a potential as a ROCK inhibitor and could be used in treatments of various pathological conditions in which ROCK plays a role. HoloMonitor M4 was used to study cell morphology (cell area, thickness, volume, and irregularity) post-treatment with various concentrations of tolbutamide and metformin.
23/01/2021	Monobutyl phthalate can induce autophagy and metabolic disorders by activating the ire1a-xbp1 pathway in zebrafish liver	Journal of Hazardous Materials	Y. Zhang et al.	China	Chemical toxicity	Cell morphology	Zebra fish (AB strain) liver cells	Primary hepatocytes extracted from adult zebra fish (AB strain)	HoloMonitor M4	Monobutyl phthalate (MBP) and dibutyl phthalate (DBP) may have similar toxicity. Thus, in this study, Y. Zhang with colleagues assess the MBP effect on zebrafish. Authors revealed that MBP introduces acute toxicity to zebra liver, leading to disturbances in metabolism while triggering cell autophagy. HoloMonitor M4 was used to observe MBP effects on hepatocyte cell morphology, including cell area, thickness, irregularity, and roughness.
06/01/2021	Snail Overexpression Alters the microRNA Content of Extracellular Vesicles Released from HT29 Colorectal Cancer Cells and Activates Pro-Inflammatory State In Vivo	Cancers	I. Papiewska-Pająk et al.	Poland	Cancer research	Cell motility	HT-29	HT-29 - human colon cancer cell line	HoloMonitor M4	Extracellular vesicles (EV) released by cancer cells have a role as intercellular communicators in the cancer metastasis process. I. Papiewska-Pajak with colleagues have studied how EV released from various types of HT-29 cells affect cancer progression. Authors have shown that HT-29 cells affected by Snail transcription factors release EVs with modified microRNA cargo and lead to more invasive cell phenotype. HoloMonitor M4 was used to study cell motility speed and migration after EV treatment.
15/12/2020	Monoclonal antibody 2C5 specifically targets neutrophil extracellular traps	mAbs	L. P. Mendes et al.	USA	Cell Research	Cell morphology	HL-60	HL-60 - human leukemia cell line	HoloMonitor M4	Neutrophil extracellular traps (NETs) are a defense mechanism against pathogens, such as bacteria, they have also been implicated as an important part of a wide range of pathologies, such as protecting tumor cells from T lymphocytes and natural killer cells; involved in acute thrombotic complications in cardiovascular and autoimmune disease, and most recently it was shown that NETs may contribute to organ damage and mortality in COVID-19. Thus, it is important to detect and remove NETs when their pathological role becomes dominant. In this publication, L. P. Mendes with colleagues have developed a method to visualize and detect NET-affected areas using 2C5 antibody modified nanocarriers. Authors have employed HoloMonitor M4 to study HL-60 cell morphology after treatment with differentiation agents.
01/12/2020	Targeted inhibition of ERα signaling and PIP5K1α/Akt pathways in castration‐resistant prostate cancer	Molecular Oncology	J. Semenas et al.	Sweden	Cancer research	Cell morphology	PC-3	PC-3 - human prostate adenocarcinoma cell line	HoloMonitor M4	As there are limited treatment options for metastatic prostate cancer (PCa), the disease progresses to castration-resistant after the initial androgen-deprivation therapy. Thus, there is a need to develop new treatment strategies to effectively treat patients. In this study, J. Semenas with colleagues have developed a novel selective inhibitor (ISA-2011B), which exhibits specific effects towards subgroups of PCas alone and in combination with tamoxifen and can provide new therapeutic opportunities. Authors have used HoloMonitor M4 to study PC-3 cell morphology changes after drug treatment.
12/11/2020	Efficient and crucial quality control of HAP1 cell ploidy status	Biology Open	T. B. Beigl et al	Norway	Cell Research	Cell morphology and cell movements	HAP1	HAP1 - near haploid cell line derived from male chronic myelogenous leukemia	HoloMonitor M4	Haploid cells are powerful tools to study gene functions and became a "workhorse" for CRISPR/Cas9 editing because a change/mutation in a single allele is enough to reveal a potential phenotype. In this study, T. B. Beigl with colleagues have aimed to check for fundamental differences between HAP1 cell cultures in the haploid versus diploid state. Authors have shown that it is extremely important to control the ploidy of HAP1 cells prior to phenotypic analyses and provide a streamlined procedure to obtain diploid cultures. Here HoloMonitor M4 was employed while comparing haploid and diploid HAP1 cell morphology, growth kinetics, and cell motility.
15/10/2020	Smart drug combinations for cervical cancer: dual targeting of Bcl-2 family of proteins and aurora kinases	Am J Cancer Res	J. Yumol et al	Australia	Cancer research	Cell morphology	HeLa	HeLa - human cervical cancer cell line	HoloMonitor M4	Current available cervical cancer treatments are not always sufficient and result in cancer recurrence. In this publication, J. Yumol and colleagues have studied how the treatment efficacy can be enhanced by combinbing Alisertib drug with Bcl-2 family anti-apoptotic protein inhibitors. Authors have shown that having this type of combination accelerates cell apoptosis during the mitotic delay. Here, HoloMonitor M4 was used to study cell cycle, e.g. mitosis time and the number of cell divisions, after drug treatments.
07/09/2020	Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays	Scientific Reports	M. Hellesvik et al	Norway	Method Development	Cell movements, Cell morphology, Wound Healing, Cell Invasion	NCL-H524, U2OS	NCL-H524 - human lung carcinoma cells; U2OS - human bone osteosarcoma epithelial cells	HoloMonitor M4	Standard 2D cell cultures do not mimic physiological conditions, resulting in research results that are not always applicable in vivo. This paper focuses on developing cell-friendly methods where cells grow in Matrigel to create a physiologically relevant environment. Using the HoloMonitor M4, Hellesvik with colleagues have shown how to quantify cell morphology, proliferation, migration, and motility in different concentrations of Matrigel. In addition, the authors have demonstrated how to quantify the initial formation of spheroids in Matrigel and the paper introduces a novel method for measuring cell invasion.
06/09/2020	SUMOylation of PUM2 promotes the vasculogenic mimicry of glioma cells via regulating CEBPD	Clinical and Translational Medicine	D. Wang et al.	China	Cancer research	Cell movements	U251, U373	U251 - human glioblastoma astrocytoma, U373 - human glioblastoma astrocytoma	HoloMonitor M4	40 % of primary intracranial tumors are glioma and is the most common primary tumor. Additionally, it is hard to treat as it is invasive and prone to recurrance. Thus in this paper, D. Wang with colleagues have studied what is the role of SUMOylation, expression, and interaction in vasculogenic mimicry, which is a blood supply channel in glioma. Authors have shown that UBE21/PUM2/CEBPD/DSG2 gene expression play a crucial role in regulating glioma vasculogenic mimicry. In this study, HoloMonitor M4 was used to study glioma cell migration capacity after transfection.
24/08/2020	Improved Autophagic Flux in Escapers from Doxorubicin-Induced Senescence/Polyploidy of Breast Cancer Cells	International Journal of Molecular Sciences	A. Bojko et al.	Poland	Cancer research	Cell proliferation	MDA-MD-231	MDA-MB-231 - Human mammary carcinoma cell line;	HoloMonitor M4	It is hypothesized that cell senescence can induce cancer recurrence and together with cell autophagy reduces chances of cancer patient recovery. In this study, A. Bojko with colleagues aimed to understand autophagy modulation in breast cancer cell induced senescence after doxorubicin treatment. Authors have shown that breast cancer cells can undergo senescence uncoupled from autophagy status. HoloMonitor M4 was used to study asymmetric cell proliferation.
02/08/2020	Monobutyl phthalate (MBP) induces energy metabolism disturbances in the gills of adult zebrafish (Danio rerio)	Environmental Pollution	Y. Tao et al	China	Chemical toxicity	Cell morphology	Zebra fish (Danio rerio) gill epithelial cells	Primary gill epithelial cells extracted from adult zebra fish (Danio rerio)	HoloMonitor M4	Monobutyl phthalate (MBP) is a primary metabolite of an environmental endocrine disruptor dibutyl phthalate, which is a threat to ecosystems. In this study, Y. Tao with coworkers have studies MBP effects on energy metabolism in the zebrafish respiratory system (gills). Authors show that MBP is acutely toxic by destroying mitochondria structure and function, disrupting ATP synthesis and ion transport, and leads to cell death. HoloMonitor M4 was used to study gill epithelial cell morphology changes exposure to various concentrations of MBP.
15/07/2020	Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry	Applied Sciences	El-Schich et al	Sweden	Cancer research	Cell morphology	Jurkat, THP-1, Hs-578T, MDA-MD-231, MDA-MB-468, T57D, Cama-1, MCF-7	Jurkat - human T-cell acute lymphoblastic leukemia; THP-1 - human monocytic cell line; Hs-578T - human mammary gland carcinoma MDA-MB-231 - Human mammary carcinoma cell line; MDA-MB-468 - Human mammary carcinoma cell line; T57D - Human mammary carcinoma cell line; Cama-1 - Human breast cancer cell line; MCF-7 breast cancer cell line;	HoloMonitor M4	Circulating Tumor Cells (CTC) are the main cause of cancer metastasis and regeneration. However, at the moment there is a lack of methods capable to detect and isolate CTC, as they are rare and heterogeneous. In this work, Z. El-Scich with colleagues have studied the morphological differences between white blood cell lines and human breast cancer cell lines. Authors have utilized HoloMonitor M4 and results show that there are significant differences in cell size-related parameters (cell area, cell volume, and cell thickness) between white blood cell lines and breast cancer cell lines, implying that HoloMonitor M4 and digital holographic cytometry can become a powerful diagnostic tool to characterize and detect CTCs in the blood.
09/07/2020	Quantifying the Rate, Degree, and Heterogeneity of Morphological Change during an Epithelial to Mesenchymal Transition Using Digital Holographic Cytometry	Applied Sciences	S. Kamlund et al.	Sweden, USA	Cancer research	Cell morphology	HDF, RPTE, L929, NMuMG, JIMT-1	HDF - human derman fibroblasts; RTPE - human renal proximal tubule epothelial cells; L929 - mouse fibroblast cell line; NMuMG - mouse mmary gland cells; JIMT-1 - human breast cancer cell line	HoloMonitor M4	Cell-based assays designed to characterize epithelial-mesenchymal phenotypic (EMP) transition usually are end-point, bulk population, or qualitative methods. Thus obtained results can miss key information on precise EMP transition process nature, kinetics, and uniformity of molecular changes depending on cell line and conditions. In this work, S. Kamlund with colleagues used digital holographic cytometry (DHC), HoloMonitor M4, to develop a tractable method for monitoring EMP in adherent mammalian cell populations. Authors have shown, that cells in different EMP transition stages exhibit different cell morphological parameters (e.g. cell eccentricity, cell hull convexity, cell optical thickness, cell roughness skewness, etc), which can be quantified and applied to calculate the degree of the EMP transition. This could potentially make DHC and HoloMonitor M4 a fast and efficient primary method to investigate EMP cell morphological changes due to experimental conditions.
09/07/2020	Salinomycin Treatment Specifically Inhibits Cell Proliferation of Cancer Stem Cells Revealed by Longitudinal Single Cell Tracking in Combination with Fluorescence Microscopy	Applied Sciences	S. Kamlund et al.	Sweden	Cancer research	Cell movements	JIMT-1	JIMT-1 - human breast cancer cells	HoloMonitor M4	Cell lines derived from tumors are comprised of a mixture of cells, which have different phenotypes. Hence, in cell population-based assays, small sub-population impact or behavior against treatment can be undetected. In this study, S. Kamlund with colleagues have used HoloMonitor M4 in combination with fluorescence microscopy to study JIMT-1 breast cancer cells for extended time and study Salinomycin effects on individual cells via longitudinal single cell tracking. Authors have shown that Salinomycin specifically targets a CD24- cell subpopulation by inhibiting its proliferation and the surviving CD24+ cell subpopulation was more epithelial-like.
07/07/2020	Subpopulations of Organoid-Forming Cells Have Different Motility	Applied Sciences	D. G. Jimenez et al	Sweden	Organoid formation	Cell morphology and cell movements	HN26	HN26 - p16+ human papillomavirus (HPV16+) cell line	HoloMonitor M4	Cancer stem cells from oropharyngeal squamous cell carcinoma can self-renew and differentiate into heterogeneous 3D structures, i.e. organoids, which have tumor cell features. However, there is a need for easy-to-use methods to understand organoid formation. In this study, D.G. Jimenez with colleagues have used HoloMonitor M4 and digital holographic microscopy to study tumor organoid development from single cells. Authors have revealed organoid formation kinetics by studying cell aggregate morphology (area, volume, thickness) changes and single cell motility.
07/07/2020	The osteogenic commitment of CD271+CD56+ bone marrow stromal cells (BMSCs) in osteoarthritic femoral head bone	Scientific Reports	D. C. Ilas et al	UK, Germany	Stem cell therapy	Cell movements	BMSC	BMSC - Bone marrow stromal cells	HoloMonitor M4	Progressive structural changes in cartilage and subchondral bone lead to osteoarthritis. In this study, D.C. Ilas with colleagues have sorted cells and used immunohistochemistry to phenotypically distinguish, osteogenically-committed bone marrow stromal cell (BMSC) subset in human osteoarthritic trabecular bone. Authors have shown that two genes, responsible for a nerve growth factor (CD271) and neural cell adhesion molecule (CD 56) expression, could be utilized as a future therapeutic target for osteoarthritis and other bone-associated pathologies. In this study, HoloMonitor M4 was used to study how BMSC cell motility is affected by cell morphology.
27/06/2020	Label-Free Classification of Apoptosis, Ferroptosis and Necroptosis Using Digital Holographic Cytometry	Applied Sciences	K. L. Barker et al	USA	Basic Cell Research	Cell morphology	501mel	501mel - human derived metastatic melanoma cell line	HoloMonitor M4	Activation of alternative programmed cell death pathways, e.g. ferroptosis and necroptosis, is a promising therapeutic strategy for cancer cells, which evade apoptosis. However, the interplay between distinct programmed cell death pathways and cancer progression is complex and can paradoxically promote the disease. In this publication, K.L. Barker with colleagues has aimed to develop a label-free, live cell imaging-based assay for classifying forms of programmed cell death with single-cell resolution. By employing HoloMonitor M4 and studying cell morphology parameter changes, authors have developed a method, which with high accuracy could classify cell death pathway in the test set for the melanoma cell line.
19/06/2020	Assembly of Epithelial Monolayers and Transmigration of Cancer Cells Captured with Phase Holographic Imaging	IEEE	Á. Nagy et al	Hungary	Cancer research	Cell morphology and cell movements	Vero and HeLa	Vero - kidney epithelial cell line HeLa - cervical cancer cell line	HoloMonitor M4	Cancer metastasis capabilities, i.e. cancerous cell ability to move from the tumor and invade healthy tissue, is a key factor for patient survival. Thus in this paper, A. Nagy with colleagues have studied how cancerous HeLa cells invade healthy Vero cell monolayer. Authors have used HoloMonitor M4 to study HeLa cell morphology and movement changes during the Vero cell invasion and added valuable information to further understanding of cancer metastasis mechanism. Additionally, they showed that HeLa cell population was comprised of invasive and non-invasive cell subpopulations.
09/06/2020	MicroRNA-362-3p Inhibits Migration and Invasion via Targeting BCAP31 in Cervical Cancer	Frontiers in Molecular Biosciences	S. Yang et al	China	Cancer research	Cells movements	HeLa	HeLa - cervical cancer cell line	HoloMonitor M4	MicroRNAs play one of the key roles in cervical cancer metastasis. Thus, in this study, S. Yang with colleagues have investigated the miR-362-3p targeting mechanism and effect. Authors have shown that miR-263-3p plays an important role in cervical cancer metastasis, as it regulates cell migration and motility by directly targeting BCAP31 protein. Hence, making miR-263-3p a potential target for cervical cancer treatment. Here, HoloMonitor M4 was used to characterize HeLa cell migration and motility after transfection.
03/06/2020	Effect of three-dimensional ECM stiffness on cancer cell migration through regulating cell volume homeostasis	Biochemical and Biophysical Research Communications	M. Wang et al	China	Cancer research	Cell morphology and cell movements	MDA-MB-231	MDA-MB-231: Human mammary carcinoma cell line	HoloMonitor M3	The extracellular matrix (ECM) stiffness has a direct effect on cancer cell homeostasis (e.g., cell volume), which is critical for the regulation of their migration. In this work, M. Wang with colleagues have modified studied extracellular matrix with tunable stiffness to study how the 3D ECM stiffness and cell media osmosity influences cell volume and their migration. Authors have revealed that 3D ECM stiffness regulates cell migration behavior through regulating cell volume homeostasis. In this study, HoloMonitor M3 was used to study cell migration and volume changes.
22/05/2020	Defining COMMD4 as an anti-cancer therapeutic target and prognostic factor in non-small cell lung cancer	British Journal of Cancer	A. Suraweera et al	Australia	Cancer research	Cell proliferation	HBEC3-KT, H460, H1975, CRL5889	HBEC3-KT - Human bronchial epithelial cells; H460 - Large cell lung carcinoma; H1975 - human non-small cell lung carcinoma CRL-5889 - non-small cell lung cancer	HoloMonitor M4	Despite current advances in cancer therapy, novel therapeutic targets and cancer therapies are needed to improve non-small lung patient prognosis and treatment. In this study, A. Suraweera with colleagues has identified that COMMD4 protein, which controls DNA transcription protein, can also function as a prognostic factor in non-small lung cancer and can be a therapeutic target. Authors have used HoloMonitor M4 to study cell proliferation changes after COMMD4 gene depletion.
27/04/2020	Exosomes derived from mesenchymal stem cells repair a Parkinson’s disease model by inducing autophagy	Cell Death and Disease	H.-X. Chen et al	China	Stem cell therapy	Cell proliferation	hucMSC	hucMSC - human umbilical cord mesenchymal stem cells	HoloMonitor M4	Human umbilical cord mesenchymal stem cells (hucMSC) can provide neuroprotection in neurodegenerative diseases, such as Parkinson's disease, treatments. However, at the moment, hucMSC applications are limited due to various reasons. In this study, H.-X. Chen with colleagues have shown that Exosomes derived from hucMSC have the potential for effective Parkinson's disease treatment because they can pass the Brain-Blood barrier. Here, HoloMonitor M4 was used to characterize hucMSC cell proliferation and helped to characterize hucMSC self-renewal and reproductive abilities.
21/04/2020	The human papillomavirus confers radiosensitivity in oropharyngeal cancer cells by enhancing DNA double strand break	Oncotarget	M. Zhang and A. M. Hong	Australia	Cancer research	Cell morphology and cell movements	OPSCC	OPSCC - Oropharyngeal squamous cell carcinoma	HoloMonitor M4	The overall better prognosis seen in Human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (OPSCC) may be related to the disease's response to radiation therapy. Radiation therapy plays an important role in the management of OPSCC, either as definitive therapy or as adjuvant therapy after surgery. In this study, M. Zhang and A. M. Hong have studied how HPV and its E6 oncoprotein affect cell morphology, radiosensitivity, and repair of radiation-induced DNA damage. Authors have shown that HPV-positive OPSCC was more radiosensitive and that E6 oncoprotein enhanced HPV-negative OPSSC cell radiosensitivity by impairing the DNA repair mechanism and enhancing apoptotic death. Authors have studied cellular response to radiation treatment with HoloMonitor M4 and observed cell migration and morphology changes.
14/04/2020	Alkyl-carbon chain length of two distinct compounds and derivatives are key determinants of their anti-Acanthamoeba activities	Scientific Reports	R. Mooney et al	Scotland, UK	Parasitic infections	Cell morphology	Acanthamoeba castellanii	Acanthamoeba castellanii - amobea	HoloMonitor M4	Acanthamoeba are free-living protists, who have opportunistic tendencies. Thus, they can affect the skin, cause severe cornea infections, or in rare cases lead to death. If Acanthamoeba damage the cornea, it can lead to sight loss. Consequently, in this publication, R. Moorey with colleagues, have studied how two molecules affect A. castellani trophozoites and showed that these molecules can be useful for preventing amoeba cornea infections, i.e. by formulating them into contact lens solution. In this study, authors have used HoloMonitor M4 to characterize how the treatment affects A. castellanii morphology: area, perimeter, and volume.
21/03/2020	N-terminal acetylation of actin by NAA80 is essential for structural integrity of the golgi apparatus	Experimental Cell Research	T. B. Beigl et al	Norway	Cell Research	Cell morphology	HAP1	HAP1 - near-haploid human cell	HoloMonitor M4	Actin is a cell skeleton and is responsible for not only cell movements, but also for molecule transport and organization of other organelles, such as Golgi aparatus. In this study, T. B. Beigl with colleagues have evaluated how actin stucture, N-terminus acetylation and actin amounts affect Golgi aparatus structure and dynamics. Authors have used HoloMonitor M4 to quantitatively assess cell morphologicy parameters - area, volume, and irregularity - change after N-terminus acetylation protein knockout.
15/03/2020	G9a-mediated repression of CDH10 in hypoxia enhances breast tumour cell motility and associates with poor survival outcome	Theranostics	F. Casciello et al	Australia	Cancer treatment	Cell motility	MDA-MB-231, MCF-7	MDA-MB-231: Human mammary carcinoma cell line; MCF-7 breast cancer cell line;	HoloMonitor M4	Epigenetic enzymes play an important role in regulating gene expression under lack of oxygen (hypoxia) in tumors. Thus during cancer metastasis, tumor hypoxia impairs cell adhesion molecule expression and allows tumor cells to detach from the primary site, migrate and establish metastases in distant areas of the body. This process is epithelial to mesenchymal transition (EMT) and leads to enhanced tumor aggressiveness, especially under hypoxia. In the present study, F. Casciello with colleagues have shown that epigenetic enzyme (G9a) accumulation in hypoxia leads to CDH10 protein transcription repression. This enhances cancer cell motility and is an integral part of hypoxia-mediated EMT in breast cancer. In this study, HoloMonitor M4 was used to precisely track single cells and quantify the total distance cells have traveled under different conditions and led authors to reach their conclusions on G9a and CDH10 role in the EMT mechanism.
10/03/2020	Antibacterial AZT derivative regulates metastasis of breast cancer cells	European Journal of Medicinal Chemistry	S. Chirumarry et al	Korea	Cancer research	Cell motility	MDA-MB-231	MDA-MB-231: Human mammary carcinoma cell line	HoloMonitor M4	Having one molecule with multiple action pathways could have a great impact on fighting antibiotic resistance and cancer metastasis. Bacteria and cancer cell membrane surfaces are similar, thus creating an opportunity for a single molecule to act as both antibacterial and anticancer agents by altering membrane tension. In the present work, S. Chirumarry with colleagues have synthesized various small molecules for antibacterial treatment and they hypothesized that their most potent compound ADG-2e may interact with cancer cell surface via electrostatic interaction and reduce the membrane tension and affect cell motility. Authors have performed cytotoxicity and cell migration and invasion studies. By employing HoloMonitor M4 and spatial single cell tracking, authors have shown, that ADG-2e compound interacts with the cancer cell surface and remarkably decreases cell migration (locomotion) compared to the untreated cells.
19/02/2020	BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis	Scientific Reports	J. Wang et al	China	Cancer research	Cell morphology and cell movements	A549, PLA-801D, GLC-82	A549 - human lung adenocarcinoma cells; PLA-801D - lung giant cell carcinoma; GLC-82 - human papillomavirus-related endocervical adenocarcinoma	HoloMonitor M4	In the absence of symptoms and effective screening programs, most lung cancers are diagnosed at advanced metastatic stages. Thus, in this study J. Wang with colleagues have explored how the metastasis mechanism (EMT) is involved in lung cancer development. Authors have demonstrated that B cell receptor-associated protein 31 (BCAP31) can be employed as a metastasis prognostic factor and used in therapeutic strategies for treating lung cancer. In this study, HoloMonitor M4 has been used to evaluate how BCAP31 protein affects cell migration, motility, optical thickness, and movement tracks. Additionally, the authors have studied the effects of BCAP31 amounts inside the cells on cell area.
29/01/2020	Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin	Frontiers in Pharmacology	S. Zhang et al	China	Cancer research	Cell morphology and cell movements	HL-60	HL-60 - human acute promyelocytic leukemia	HoloMonitor M4	Chemotherapy is one of the main treatments for cancer patients. However, some patients develop chemotherapeutic agent resistance, thus impeding treatment efficiency. One of the hypotheses is that chemotherapeutic resistance and immune responses are related to each other, however, the relationship between them is still unclear. In this study, Sh. Zhang with colleagues have explored the neutrophil's contribution to chemotherapeutic resistance and berberine-regulated cancer cell sensitivity to doxorubicin (DOX). Authors have shown that DOX alone induces HL-60 cell differentiation to N2 type neutrophils and induces chemotherapeutic resistance. When DOX is administered in combination with berberine, HL-60 cells differentiated into N2 type neutrophils and stimulated HL-60 cell immune clearance. In this study, authors have used HoloMonitor M4 to evaluate HL-60 cell differentiation to N1 and N2 type neutrophils by evaluating different phenotypic characteristics in means of morphology and motility.
21/01/2020	Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry	Applied Sciences	M. Patel et al	Sweden, Czech Republic	Cancer treatment	Cell morphology and cell movements	MCF-7 and MDAMB231	MCF-7 breast cancer cell line; MDA-MB-231: Human mammary carcinoma cell line;	HoloMonitor M4	In order to find a way to increase breast cancer patient prognosis, scientists need to understand cell behavior and metastasis. One of the possible tools in the efficient diagnosis is sialic acid-molecularly imprinted polymers (SA-MIPs). The aim of this paper was to study the effects of SA-MIPs on cell morphology and behavior. Using HoloMonitor M4 M. Patel with colleagues show SA-MIP can change cell motility (slower down MCF-7 cells and increase MDAMB231 cell speed), cell morphology for both cell lines.
23/12/2019	Synthesis and biological evaluation of 4,5,6,7‐tetrahydrothieno[2,3‐c]pyridine–based β‐aminonitriles and their derivatives: β‐amino carboxamides, (thio)ureas, and tetracycles	Journal of Heterocyclic Chemistry	R. Madacsi et al.	Hungary	Drug reseach	Cell morphology	A549	A549 - human lung adenocarcinoma cells	HoloMonitor M3
03/12/2019	Development of label-free biophysical markers in osteogenic maturation	Journal of the Mechanical Behavior of Biomedical Materials	A. Bartolozzi et al	Italy	Cell Research	Cell morphology	hFOB 1.19	hFOB - human foetal osteoblasts	HoloMonitor M3	Morphological and mechanical changes in living cells indicate functionally associated processes, such as cell maturation or death. In this study A. Bartolozzi with colleagues characterized the maturation of human fetal osteoblasts. The authors highlight that non-invasive methods, such as digital holography, atomic force microscopy, and TIE, have the ability to identify effective label-free biomarkers, which help to characterize cell biomarkers required for this fundamental process. Authors have used HoloMonitor M3 to quantify cell morphology (among others, area, perimeter, and volume) changes during hFOB maturation.
26/11/2019	Autologous tumor‑derived microvesicles influence gene expression profiles and enhance protumorigenic chemotactic potential, signal transduction and cellular respiration in gastric cancer cells	International Journal of Oncology	R. Szatanek et al	Poland	Cancer research	Chemotaxis	GC1415	GC1415 - gastric cancer	HoloMonitor M4	Tumor‑derived microvesicles (TMVs) interact with cells within the immune system. In the present study, R. Szatanek with co-workers have studied the effects of autologous‑TMVs (auto‑TMVs) on gene expression. Chemotaxis, intercellular signaling and cellular metabolism were examined in cells of the gastric cancer (GC) cell line. Authors have used HoloMonitor M4 to perform chemotaxis assay and showed that Auto-TVMs led to 75 gene overexpression and 96 gene underexpression, which affect, among others, chemotaxis.
21/11/2019	Colony Formation, Migratory, and Differentiation Characteristics of Multipotential Stromal Cells (MSCs) from “Clinically Accessible” Human Periosteum Compared to Donor-Matched Bone Marrow MSCs	Stem Cells International	H. E. Owston et al	UK	Stem cell therapy	Cell morphology and cell movements	Primary BM and primary MSC	Primary BM - primary human bone marrow cells Primary MSC - primary human multipotential stromal cells	HoloMonitor M4	Periosteum, a highly vascular and multipotential stromal cell- (MSC-) rich tissue, is vital for fracture healing. In this study, H. E. Owston et al aimed to compare human periosteum and donor-matched iliac crest BM MSC content. They also characterized MSCs in terms of colony formation, growth kinetics, phenotype, cell migration patterns, and trilineage differentiation capacity. Authors have used HoloMonitor M4 to show that in early culture, periosteum cells were less migratory at slower speeds than BM cells. Live cell tracking allowed to determine unique morphological and migration characteristics of periosteal MSCs that can be used to develop novel bone graft substitutes.
17/11/2019	KLF2+ stemness maintains human mesenchymal stem cells in bone regeneration	Stem Cells	Y. Zhou et al	China	Stem cell therapy	Cell movement and wound healing	hMSCs and HUVEC	hMSC - human mesenchyman stem cells HUVEC - human umbilical vein endothelial cells	HoloMonitor M4	Authors have studied how zinc finger protein presence influences stem cell capabilities to form blood vessels (undergo angiogenesis). In one part of this study authors have knocked down zinc finger proteins (KLF2) in mesenchymal stem cells (MSC) and in co-culture with HUVEC cells studied cell movements using HoloMonitor M4 (Figure 7C and 7D, and Video S5 and S6). Using HoloMonitor M4, Y. Zhour with colleagues, in real-time, have shown that absence of zinc finger proteins influences MSC cell motility speed and motility distance.
01/10/2019	Synthesis of a dihalogenated pyridinyl silicon rhodamine for mitochondrial imaging by a halogen dance rearrangement	Beilstein J. Org. Chem.	J. Matthias et al.	Germany	Flourofore synthesis	Cell proliferation	U2OS	U2OS - human bone osteosarcoma epithelial cells	HoloMonitor M4	Silicon xanthenes and silicon rhodamines are attractive as new fluorescent labels in the live sciences. Thus their development is at the high peak, especially for applications as a stains for use in super-resolution microscopy. In this publication, J. Matthias with co-workers have succesfully synthesised silicon rhodamine derivative, which had a high quantum yield and is suitable for live cell STED-nanoscopy, as it was non-toxic to studied cells. By employing HoloMonitor M4 authors have shown that new silicon rhodamine dye is not cytotoxic as cell prolifetion, including cell division time, cell count, and cell confluence, were uneffected.
16/09/2019	Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In Vivo and Three-Dimensional Models over the Petri-Dish	Cells	R. Alföldi et al.	Hungary	Cancer research	Phase contrast	A549	A549 - human lung adenocarcimona cell line	HoloMonitor M3	In this study authors have characterized lung cancer markers from cells cultured in two-dimensional (2D) and three-dimensional (3D) fashion. They have concluded that 3D cell cultures should be used for drug screening as these structures are more similar to the in vivo tumor conditions. R. Alfödi et al have used phase contrast functionality in HoloMonitor M3 to evaluate different culture conditions on A549 cells after 4 and 9 days post seeding.
04/09/2019	Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies	Theranostics: Methods and Protocols	L. V. Croft et al.	Australia	Cancer research	Cell proliferation, cell morphology	HeLa	HeLa - human cervical cancer cell line	HoloMonitor M4	L. Croft with co-authors have prepared a protocol on how to evaluate cancer drug affects by using non-invasing digital holographic imaging, i.e. HoloMonitor M4. Authors describe the use of the DHI platform HoloMonitorM4 to evaluate the effects of novel, targeted cancer therapies on cell viability and proliferation using the HeLa cancer cell line as a model. They present single cell tracking and population-wide analysis of multiple cell morphology parameters.
28/08/2019	Propofol prevents oxidative stress and apoptosis by regulating iron homeostasis and targeting JAK/STAT3 signaling in SH-SY5Y cells	Brain Research Bulletin	Y. Zhang et al.	China	Neurodegenerative disorders	Cell count	SH-SY5Y	SH-SY5Y - human neuroblastoma cell line	HoloMonitor M4	Y. Zhang with colleagues have demonstrated a new antioxidation mechanism of propofol, an intravenous anesthetic. Propofol could protect against nerve cell injury, induced by overload of iron through regulating iron metabolism and inhibiting stress and inflammation reaction pathways. Authors have used HoloMonitor M4 to confirm that propofol suppressed cell apoptosis by evaluating cell count.
30/07/2019	Adipocytes Migration is Altered Through Differentiation	Microscopy and Microanalysis	M. Lustig et al.	Israel	Cell Research	Cell migration and motility	3T3-L1	3T3-L1 are mouse embryonic preadipocytes	HoloMonitor M4	Preadipocyte differentiation from elongated structures to rounded adipocytes is adipogenesis. During the process cell cytoskeleton undergoes changes, which in turn alterate single-cell motility. In this work M. Lusting with colleagues for the first time evaluate differences between the motility of nondifferenciated preadipocytes and differentiated mature adipocytes using hologprahic imaging. Authors have used HoloMonitor M4 to show that preadipocytes move faster and further than adipocytes, idicating that cell cytoskeleton is altered during cell differentiation.
14/07/2019	Unravelling the role of trophoblastic-derived extracellular vesicles in in regulatory T cell differentiation	International Journal of Molecular Sciences	A. F. Kovacs et al.	Hungary	Cell Research	Cell migration and motility	Regulatory T cells (T reg cells)	Primary human immune suppressive cells	HoloMonitor M4	A. F. Kovacs et al has studied de novo differentiation of regulatory T cells by trophoblast-derived extracellular vesicles. Authors show that HSPE1 is a useful marker of Treg subtype characterization and have an important role in Treg cell expansion in vitro. Authors have employed HoloMonitor M4 to study Treg cell viability and showed positive migratory and motility capacity.
12/07/2019	Exploring the Natural Piericidins as Anti-Renal Cell Carcinoma Agents Targeting Peroxiredoxin 1	Journal of Medical Chemistry	X. Zhou et al	China, USA	Cytotoxicity	Cell proliferation	ACHN	ACHN - human renal carcinoma cells	HoloMonitor M4	X. Zhou with co-workers have evaluated 27 piericidin products against renal cell carcinoma (RCC). Authors have shown that piericidins provide a novel scaffold for further development of anti-RCC agents, and the new action mechanism targeting peroxiredoxin 1 may improve the existing renal cancer drugs. In this study, authors have used HoloMonitor M4 to study ACHN cell proliferation after exposure to 50 nM of piericidin.
21/06/2019	Uptake of nanowires by human lung adenocarcinoma cell	PLoS ONE	L. Abariute et al	Sweden	Cytotoxicity	Cell migration and motility	A549	A549 - human lung adenocarcinoma cell line	HoloMonitor M4	Semiconductor nanowires are increasingly used in optoelectronic devices. Thus, in this work, L. Abariute et al have invertigated the effects of gallium phosphide nanowires on human lung adenocarcinoma cells. Authors have showed that cells internalize the nanowires and that the nanowires have no effect on cell proliferation rate, motility, viability, and intracellular ROS levels. Additionally, authors have studied possible nanowire internalization pathways. In this work HoloMonitor M4 was used to study the effects of internalized nanowires on cell motility and migration.
14/05/2019	Complex approach to the estimation of cytotoxicity of ribosome-inactivating proteins on the cell model in vitro (in Russian)	Medicine of Extreme Situations	E. O. Sinegubova et al	Russia	Cytotoxicity	Cell morphology	HEK293	HEK293 - human embryonic kidney cells	HoloMonitor M4	E.O. Sinegubova with co-workers have studied viscumin cytotoxicity in an integrated approach by combining colorimetric assay with phase holographic imaging. By employing MTT assays authors have determined IC50 viscumin value. With help of HoloMonitor M4 authors have showed how viscumin at IC50 concentration affect cell morphology and cell confluency. Authors conclude that the combination of two methods gives an opportunity to evaluate several parameters at the same time and let to better understanding of viscumin effects on cells.
13/05/2019	The long noncoding RNA TP73‐AS1 promotes tumorigenicity of medulloblastoma cells	International Journal of Cancer	M. Varon et al	Israel, Germany	Cancer research	Cell migration and motility, wound healing	DAOY, UW 228.2	DAOY - human primary medulloblastoma cell line UW228.2 - pediatric primary brain tumor cell line	HoloMonitor M4	M. Varon with co-authors focused their research on medulloblastoma - the most common malignant brain cancer in children. Authors have studied what functions a long noncoding RNA, specifically TP73-AS1, perform. In this study, results show that the high expression of TP73-AS1 can be associated with poor outcome in some patients. Furthermore, silencing on TP73-AS1 in tumor cells induce cell apoptosis and reduce cell proliferation and motility rates in vitro, and prolonged sur.vival of tumor bearing mice in vivo. Authors have employed HoloMonitor M4 to evaluate TP73-AS1 effect on cell motility and showed that it promotes migration of tumor cells, DAOY and UW288.2
17/04/2019	ITGA2 as a potential nanotherapeutic target for glioblastoma	Scientific Reports	Guo P. et al	USA	Cancer research	Cell migration and morphology	A172, U87	A172 - human glioblastoma cell line U87 - human primary glioblastoma cell line	HoloMonitor M4	Most common and deadly brain cancer type in adults is glioblastoma. Thus, there is an urgent demand to develop an effective targeted therapeutic treatment. P. Gao with colleagues have identified and studied the ITGA2 cell antigen as a potential therapeutic target for glioblastoma. Their research results show that ITGA2 is more expressed in human glioblastoma cells and antigen blocking impedes cell migration. Additionally, authors have developed specified liposomes, which selectively deliver doxorubicin to the cells and inhibit ITGA2. Authors have used HoloMonitor M4 to evaluate the effects of ITGA2 antibody and IgG-conjugated liposome treatment on A172 and U87 cell motility.
01/04/2019	In vitro SOD-like activity of mono- and di-copper complexes with a phosphonate substituted SALAN-type ligand	Chemico - Biological Interactions	Szekacs I. et al	Hungary, Poland, Japan, Germany	Cytotoxicity	Cell morphology	MC3T3-E1	MC3T3-E1 - osteoblast-like cells	HoloMonitor M4	Malfuntioning superoxide dismutase (SOD) enzymes are linked to the imbalanced levels of radicals and various pathological conditions, including cancer. Thus, Szekacs with co-workers have studied two SOD mimiking agents functions, reactivity and toxicity. Authors have found that one analog, Cu[L], can be a promising copper based platform for biochemical and phamaceutical applications. HoloMonitor M4 was used to study SOD analog effects MC3T3-E1 cell morphology and proliferation changes for 72 hours after exposure. Authors have shown that Cu[L] can be tolerated by cells in vitro at high concentrations, while Cu2[L] induced cell changes, inplying cytotoxic effects.
29/03/2019	Momordicoside G Regulates Macrophage Phenotypes to Stimulate Efficient Repair of Lung Injury and Prevent Urethane-Induced Lung Carcinoma Lesions	Frontiers in Pharmocology	Du Z. et al	China	Tissue regeneration	Cell morphology	RAW 264.7	RAW 264.7 - murine cell line, macrophages from blood	HoloMonitor M4	In this study, Z. Du et al. have explored the contributions of macrophages to the effects of momordicoside G on lung injury and carcinoma lesions. Momordicoside G is a bioactive component from Momordica charantia, which is used in various traditional Chinese, Southeast Asian, and South African remedies. Authors have showed that momordicoside G can selectively suppress M1 type macrophages to maintain M2 type macrophages for lung injury repair and preventinflammation-related lung carcinogenesis and in combination they promote lung injury repair. HoloMonitor M4 was employed to evaluate momordicoside G effects (at 40 µM concentration) on macrophage morphology in vitro. The results showed that M1 type macrophage volume and area decrease and cells underwent apoptosis, while M2 type macrophages were unaffected.
28/03/2019	Effects of GADL1 overexpression on cell migration and the associated morphological changes	Scientific Reports	Wu T-N. et al	Taiwan	Bipolar disorder treatment	Cell migration and morphology	SH-SY5Y	SH-SY5Y - human neuroblastoma cell line	HoloMonitor M4	Lithium is the first-line mood stabilizer for bipolar disorder treatment. However, it has been shown that the treatment effectivness depends on patient decent. In this paper, T.-N. Wu with co-workers have studied how GADL-1 protein (expressed in Han Chinese bipolar patients) affects lithium treatment response in human neuroblastoma cells. HoloMonitor was extensively used in this study to monitor cell count, cell migration, and cell morphology, e.g. area, volume, thickness, perimeter length, irregularity, and eccentricity, changes in time. From obtained results, authors have concluded that cell migration and related morphological changes might provide good indicators for the lithium treatment sensitivity.
27/03/2019	Synthesis, Characterization, and Photodynamic Therapy Activity of 5,10,15,20-Tetrakis(carboxyl)porphyrin	Bioorganic & Medicinal Chemistry	Zhang Z. et al	China	Cancer research	Cell morphology	A549, HeLa, HepG2, BEAS-2B	A549 - human lung adenocarcinoma cell line; HeLa - cervical cancer cell line; HepG2 - human liver cancer cell line; BEAS-2B - human bronchial epithelium cell line	HoloMonitor M3	Photodynamic therapy is used as a clinical therapeutic method for solid tumors and nonmalignant lesions. It is based on water-soluble porphyrins and their properties, such as water solubility, low dark toxicity, high affinity for tumor tissue, and strong DNA binding. In this article, Z. Zhang et al. have studied the anionic porphyrin (1-Zn) activity. Authors have concluded that 1-Zn exhibits good photonuclease activity and might serve as a potential photosensitizer for lung cancer cells. HoloMonitor was used to track A549 cell volume decrease after exposure to 1-Zn in combination with light irradiation.
22/03/2019	Digital Holographic Cytometry: Macrophage Uptake of Nanoprobes	Imaging & Microscopy	Sternbæk L. et al	Sweden	Cytotoxicity	Cell morphology, proliferation	RAW 264.7	RAW 264.7 - murine cell line, macrophages from blood	HoloMonitor M4	Various nanoprobes are designed to have drug-delivery or detection properties. However, as a foreign bodies in human blood circulation, nanoprobes can potentially induce inflamatory response and active macrophages. In this study, L. Stenbæk et al. have employed HoloMonitor M4 to study the effects of nanoprobes (molecularly imprinted polymers targeted to sialic acid) on macrophage cell line, RAW 264.7 by evaluating cell morphology (cell area, volume, and thickness) and cell count. Authors have shown that neither the HoloMonitor M4 nor nanoprobes affected the physiological functions of macrophages.
22/03/2019	Whole-Cell Multiparameter Assay for Ricin and Abrin Activity-Based Digital Holographic Microscopy	Toxins	Makdasi E. et al	Israel	Cytotoxicity	Cell morphology	HeLa and Vero	HeLa - cervical cancer cell line; Vero - african green monkey kidney epithelium cell line	HoloMonitor M4	Ricin and abrin are ribosome-inactivating proteins, which can be easily and cheaply extracted, thus they can become potential biothreat agents. In this study, E. Makdasi et al. have developed a ricin and abrin activity assay based on the digital holographic microscopy (HoloMonitor M4) evaluation of cells following intoxication. Effects of ricin and abrin intoxication on cell lines were characterized by a decrease in cell confluence, reduced cell division, and smaller cell counts compared to untreated cells. Additionally, changes in cell morphology, such as decrease in cell area, perimeter, irregularity, and effective-calculated volume, and an increase in optical thickness and roughness. Authors conclude that HoloMonitor M4 can be applied for early and sensitive detection of ricin and abrin, as well as other toxins and substances, effects in cellular features upon toxin-mediated cell death processes.
18/03/2019	Emodin regulates neutrophil phenotypes to prevent hypercoagulation and lung carcinogenesis	Journal of Translational Medicine	Li Z. et al	China	Cancer research	Cell morphology	HL-60 and LLC	HL-60 - human acute promyelocytic leukemia; LLC - Lewis lung carcinoma	HoloMonitor M4	Emodin is a natural bioactive compound present in various Chinese medicinal herbs. It has multiple functions, including anti-proliferative, anti-tumor, and anti-inflammation. This study investigated the effects of emodin on neutrophil contributions to hypercoagulation and carcinogenesis. Z. Li et al. have suggested novel emodin functions, which led to selectively suppresing N2 type neutrophils and preventing hypercoagulation and lung carcinogenesis. Authors have used HoloMonitor M4 to study neutrophil volume change after emodin exposure.
09/03/2019	Preparation of fibrin hydrogels to promote the recruitment of anti-inflammatory macrophages	Acta Biomaterialia	Tanaka R. et al	Japan	Tissue regeneration	Cell morphology	Monocytes/Macrophages	Mouse bone marrow-derived monocytes/macrophages, human monocyte-derived macrophages (HMDMs)	HoloMonitor M4	During tissue regeneration macrophages, especially M2 type, play critical role in a successful healing process. Polymerized fibrin, fibrinogen, acts as a scaffold, into which macrophages can infiltrate to heal the injury. In this paper R. Tanaka et al. have fabricated fibrin hydrogel scaffolds, which promote anti-inflammatory polarization of both mouse and human macrophages. Authors have shown that fibrin hydrogel alone exerts adequate promoting effect on macrophage recruitment (M2 type) when compared to a fibrin hydrogel loaded with sphingosine-1-phosphate receptor 1 selective agonist, SEW2871. In this study, HoloMonitor M4 was used to study the ability of human-monocyte-derived macrophage to degrade fibrin hydrogel. Authors have shown that most of the macrophages did not adhere to the surface, and the decomposition of the fibrin hydrogel was performed by many nonadherent aggregates in cooperation with a small number of adherent cells.
04/03/2019	Advancing methods for the analysis of glioblastoma cell motion using quantitative time lapse holographic imaging and cellular tomography	SPIE BiOS	Luther E. et al	USA	Cancer research	Cell movement	Glioblastoma U87	Gliobastomas are brain cancer cells	HoloMonitor M4	Glioblastomas are aggressive brain cancer and patients are given poor survival prognosis. As the cells form long-term cellular associations, understanding their motility is challenging. E. Luther et al. have used quantitative time lapse holography imaging and cellular tomography to study cell motility, cell-cell interactions and cell network forming. Authors have used HoloMonitor M4 to analyze overall cell population movement by creating 4D images. First, separating X and Y information, then adding Z as time, and finally, cell thickness (or voxel brightness) as a fourth dimension. The latter one was used to study cell proliferation and differentiate between the phases of the cell cycle, along with the separation of mitotic cells from interphase cells. Additionally, E. Luther with colleagues have used a wound healing assay to test two drug capability to immobilize cells.
21/02/2019	Metabolism of diethyl phthalate (DEP) and identification of degradation intermediates by Pseudomonas sp. DNE-S1	Ecotoxicology and Envoronmental Safety	Tao Y. et al	China, Hong Kong	Biodegradation	Cell morphology	Pseudomonas sp. DNE-S1	Pseudomonas sp. DNE-S1 is a strain able to degrate DEP from contaminated soil in Zhaodong landfills, China.	HoloMonitor M4	Due to phthalic acid ester release into the ecosystems concerns are raised about their ecological and human health effects. It has been shown that diethyl phthalate (DEP) are able to induce cancer in animals and humans and have effects on the formation of female reproductive organs. Y. Tao et al. have screened a new strain Pseudomonas sp. DNE-S1, which can degrade DEP from contaminated soil in Zhaodong landfill, China. Authors have evaluated how the strain uses DEP as a carbon source in different environment conditions, as well as changes in cell morphology. HoloMonitor M4 was used to analyze the effect of metal ions on the cell morphology, including area, thickness, and volume. Y. Tao et al. have shown that after adding metal ions strain morphology changed significantly: cells became higher and the thickner, concluding that different metals have different effect on cell morphology.
02/02/2019	The photocytotoxicity effect of cationic sulfonated corrole towards lung cancer cells: in vitro and in vivo study	Lasers in Medical Science	Zhang Z. et al	China	Cancer research	Cell morphology	A549	Human adenocarcinoma lung cells	HoloMonitor M3	Z. Zhang with co-authors have studied the effects of cationic sulfonated corrole after irradiation with light on lung cancer in vitro and in vivo. Authors showed that synthesized chemical has a potential to be employed as a potential photosensitizer as it can induce A549 cell apoptosis by increasing ROS levels and activating mitochondrial apoptosis pathway. In this study, HoloMonitor M3 was used to study A549 cell morphology changes in time for 24 hours after exposure to corrole and light irradiation and controls for 1 hour. Authors have shown the decrease in A549 cell volume after 1 hour light illumination and corrole administration.
17/01/2019	Biomimetic dextran-based hydrogel layers for cell micropatterning over large areas using FluidFM BOT technology	Langmuir	A. Saftics et al.	Hungary	Biosensors	Cell viability, adhesion, cell migration	HeLa	HeLa - cervical cancer cell line	HoloMonitor M4	A. Saftics with co-authors have studied micropaterning for possible biosensor applications. They have used HoloMonitor to monitor cell adhesion. Additionally, authors have employed HoloMonitor M4 to study cell proliferation and cell migration in and out the paterned stuctures.
14/01/2019	Hippo pathway controls cell adhesion and context-dependent cell competition to influence skin engrafment efficiency	The FASEB journal	M. Nishio et al.	Japan	Skin engraftment	Cell adhesion	Pia arachnoid mesothelial cells, MDCK, NIH3T3, and CaSki cells	Mouse keratinocytes (PAM), dog kidney (MDCK), and mouse fibroblast (NIH3T3) cells, Human cervix cells from metastatic site (CaSki)	HoloMonitor M4	The authors have studied yes-associated protein -1 (YAP1) influence to cell competition and show that the effect is context dependent. Authors conclude that YAP1 activation controls cell cempetition in part by decreasing cell adhesion. In this study HoloMonitor was used to evaluate cell contact area and monolayer height after transfection.
01/01/2019	Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression	International Journal of Medical Sciences	C.-C. Chang et al.	Taiwan	Obesity	Cell count	3T3-L1	3T3-L1 are preadipocytes	HoloMonitor M4	The authors of the paper have studied lipopolysaccharide (LPS) effects in adipocytes and preposed a model for LPS regulated preadipocyte proliferation and adipogenesis. Holomonitor M4 was used to count cell number after various treatments.
16/12/2018	Moving into a new dimension: Tracking migrating cells with digital holographic cytometry in 3D	Cytometry Part A	A. Gjörloff Wingren	Sweden	Cancer research	Motility, migration, morphology	N/A (commentary)	N/A	HoloMonitor M4	Commentary article discussing the fundamental role of cell movement studies in cancer research. Advantages of the HoloMonitor Track Cells and Wound Healing modules over the transwell migration and invasion assays are highlighted, and includes the possibility to use the cells in experiments for other purposes after completing the imaging. In addition to single cell tracking, the HoloMonitor technology also benefits from the fact that morphology analysis can be performed of each cell. The author concludes that this indeed opens up for almost unlimited possibilities to perform cell morphology analysis using this methodology, since each image is very rich in cellular information.
27/11/2018	Nuclear respiratory factor 1 acting as an oncoprotein drives estrogen-induced breast carcinogenesis	Cells	J.K. Das	USA	Cancer research	Spheroids formation, migration	MCF-10A, MCF-7, and MDA-MB231	MCF-10A human breast epithelia cells, MCF-7 breast cancer cell line, MDA-MB231 human breast adenocarcinoma	HoloMonitor M4	Findings of this study provide a new understanding for the mechanism of estrogen-induced malignant transformation of MCF10A cells (human breast epithelia cells) by NRF1 (nuclear respiratory factor 1). HoloMonitor was used for imaging of formation of tumor spheroids used in the study, and for assessment of migration. The authors hope that their findings may open new avenues for therapies against breast cancer.
21/11/2018	Lack of LTβR Increases Susceptibility of IPEC-J2 Cells to Porcine Epidemic Diarrhea Virus	Cells	T. Altawaty et al.	China	Cell research	Cell cycle	IPEC-J2	IPEC-J2 are intestinal porcine enterocyte cell line	HoloMonitor M4	T. Altawaty with colleagues have investigated LTbR receptor role in maintaining cellilar functions of IPEC-J2 cell line, during porcine epidemic diarrhea virus (PEDV) infection. Authors have showed that LTbR plays a key role during cellular response to PEDV infection. In this study, HoloMonitor M4 was used to study cell morphology (cell volume and area) changes for 72 hours and helped authors to conclude that the lack of LTbR repector leads to cell apoptosis.
28/10/2018	The connection between the cardiac glycoside‐induced senescent cell morphology and Rho/Rho kinase pathway	Cytoskeleton	Y. D. Simay et al.	Turkey	Cancer research	Cell morphology	HeLa	HeLa, cervical cancer cell line	HoloMonitor M4	The study focused on drug-induced senescence and morphological changes HeLa cells undergo during the process. HoloMonitor M4 was used to study the effects of two compounds on cell morphology, including cell area, volume, thickness, and shape irregularities. Using HoloMonitor authors showed that cell area and volume changes during incubation with inhibitors.
21/10/2018	Evaluation of holographic imaging cytometer HoloMonitor M4® motility applications	Cytometry Part A	Y. Zhang and R. L. Judson	USA	Cancer research	Motility, migration, morphology	1205Lu and WM793	Human melanoma cell lines, 1205Lu metastatic, WM793 non-metastatic	HoloMonitor M4	The HoloMonitor software modules for cell tracking and wound healing analysis were evaluated and compared to the more conventional methods transwell migration and transwell invasion. Both HoloMonitor modules were found to be well-correlated with established standards, yielded reproducible results, and at the same time offered distinct advantages. The wound healing assay was the most tractable and automated method with good reproducibility, while the cell tracking module enabled identification of hypermobile subpopulations.
21/10/2018	Actin polymerization and cell motility are affected by NAA80-mediated posttranslational N-terminal acetylation of actin	Communicative & Integrative Biology	H. Aksnes et al.	Norway	Basic Cell Research	Motility, migration	HAP1	HAP1 - near-haploid human cell	HoloMonitor M4	It is well known that actin plays an essential role in muscle cell contraction, and cellular functions like skeleton/structure-function, organelle trafficking and cell migration. The authors studied how NAA80, by acetylating actin’s N-terminus, acts as a natural brake in the cells. Single-cell identification and tracking of movement was performed with HoloMonitor, and single-cell analysis used to demonstrate the power of this mechanism.
10/10/2018	Single cell analysis of proliferation and movement of cancer and normal-like cells on nanowire array substrates	J. Mater. Chem. B	Z. Li et al.	Sweden	Nanomaterials and cell interactions	Motility, migration, cell cycle	MCF10A, JIMT-1	MCF-10 A: human breast epithelia cells, JIMT-1 human breast cancer cells	HoloMonitor M4	The article presents a single cell analysis of cell cycle and cell movement parameters of cells seeded on nanowires using HoloMonitor M4 for time-lapse imaging. Nanowires are presently investigated in the context of various biological and medical applications. In general, these studies are population-based, which results in sub-populations being overlooked. Altogether, this study highlight the importance of performing single cell analysis to increase our understanding of the versatility of cell behavior on different substrates.
26/09/2018	Comparative cell biological study of in vitro antitumor and antimetastatic activity on melanoma cells of GnRH-III-containing conjugates modified with short-chain fatty acids	Beilstein J. Org. Chem.	E. Lajko et al.	Hungary	Cancer Research	Migration, morphology, adhesion	A2058	A2058, melanoma cell line	HoloMonitor M4	The article studies various peptide hormone-based derivates, in this case GnRH-R, for tumor therapy. HoloMonitor M4 was used to evaluate the effects of the derivatives on melanoma cell morphology, including cell area, thickness, eccentricity, hull convexity, and irregularity. Additionally, authors employed HoloMonitor to study cell motility, migration, and speed in order to evaluate peptide derivative ability to immobilize cells and prevent spreading and possible metastasis development.
25/09/2018	Ulleungdin, a lasso peptide with cancer cell migration inhibitory activity discovered by the genome mining approach	J. Nat. Prod.	S. Son et al.	Korea	Cancer Research	Motility, migration	A549	Human lung carcinoma	HoloMonitor M4	Ulleungdin, a new lasso peptide was isolated from Streptomyces KCB13F003. Structural analysis of ulleungdin, as well as cancer cell invasion and migration were studied. Ulleungdin affects invasivity as measured by a transwell assay, while there was a low and not increasing cytotoxicity. Using HoloMonitor is was shown that Ulleungdin inhibited motility and migration of human lung carcinoma A549 cells. Increasing concentrations of Ulleungdin increasingly affected cell motility.
24/09/2018	TRPM2 modulates neutrophil attraction to murine tumor cells by regulating CXCL2 expression	Cancer Immunology, Immunotherapy	M. Gershkovitz et al.	Israel	Immunoncology	Molitily, migration	4Ti, AT3, TRPM2, LLC, human neutrophils	4T1 and AT3 mouse breast tumor cells, LLC=Lewis Lung Carcinova	HoloMonitor M4	The tumor microenvironment in vivo is not limited to the tumor cells themselves but also includes many kinds of immune cells. In this study the researchers explored cytotoxic effects of neutrophils in a co-culture with murine tumor cells. To exert their cell killing effect the neutrophils must reach the target cells, i.e. tumor cells. Using the HoloMonitor cell motility and migration assays the scientists could explore how the ability of the neutrophils to approach the cancer cells was affected during various conditions. Their findings are also supported and visualized in an associated time-lapse movie.
09/09/2018	Translational medicine in pulmonary-tenal crosstalk: Therapeutic targeting of p-cresyl sulfate triggered nonspecific ROS and chemoattractants in dyspneic patients with uremic lung injury	J. Clin. Med.	J.-F. Chang et al.	Taiwan	Uremic Lung Injury	Cell counting	A549	Lung cancer cells	HoloMonitor M4	Chronic kidney disease causes kidney failure, but there are also many side effects, e.g. respiratory distress called uremic lung injury. P-Cresyl sulfate (PCS) is produced when proteins ferment in intestinal bacteria, and accumulates in patients with chronic kidney disease. The authors found an effect of PCS on lung cancer cells as measured by cell count using HoloMonitor. The higher the concentration the more effect, until cell death was initiated. Authors have tried to inhibit the effect of PCS with several different substances and used cell count here as well.
06/09/2018	Imidazole compounds for protecting choroidal endothelial cells from complement injury	Scientific Reports	S. Zeng et al.	USA	AMD (age-related macular degeneration)	Cell death	RF/6A	Promate endothelial cells (nonhuman)	HoloMonitor M4	AMD (age-related macular degeneration) is a common and serious eye disease, affecting mostly elderly people. Since the disease is associated with increased activity of the complement system and cell death of endothelial cells, it was hypothesized that inhibition of the complement system can help in AMD. The authors found two certain small molecules that seemed to protect cells from the kind of complement-induced injury seen in AMD. Using HoloMonitor M4 the authors could estimate the cell status; cells surface roughness was increased when cells were treated with complement factors, and reduced when pretreated with such molecules.
29/08/2018	Nexilin/NEXN controls actin polymerization in smooth muscle and is regulated by myocardin family coactivators and YAP	Scientific Reports	B. Zhu et al.	Sweden	Basic physiology	Migration, motility, confluence	SMS	Human smooth muscle cells	HoloMonitor M4	The authors hypothesized that Nexilin associates with dense bodies and dense bands in SMCs (smooth muscle cells). The results indicate that Nexilin is central for SMC structure and function. Cell migration studies was performed using a scratch assay, and the cell confluence as well as the speed of the cell motility in the wound were followed over time using HoloMonitor.
22/08/2018	Live-stream characterization of cadmium-induced cell death using visible CdTe-QDs	Scientific Reports	S. Filali et al.	France	Method development, cell death studies	Morphology (cell area, volume and thickness)	human synoviocytes	Rheumatoid arthritis - fibroblast-like synoviocytes grown from synovial tissue of three patients	HoloMonitor M4	The authors compared various methods to study cell death processes and characterization of apoptosis.Using HoloMonitor the authors could confirm apoptosis by the quantification of morphological parameters, i.e. reduced surface area and increased cell thickness.
09/08/2018	Hexagonal boron nitride functionalized with Au nanoparticles—properties and potential biological applications	Nanomaterials	M. Jedrzejczak-Silicka et al.	Poland	Biocompatibility	Proliferation, morphology	L929, MCF-7	L929: mouse fibroblasts, human breastcancer	HoloMonitor M4	Due to its chemical properties hexagonal boron nitride with gold nanoparticles may be an interesting material for cancer drug delivery etc. In this study, potential biomedical applications and cytotoxicity of the material was evaluated. HoloMonitor M4 was used as a tool to determine the effects on cell proliferation and cell morphology over time, after exposue to the material.
01/08/2018	General cytotoxicity and its application in nanomaterial analysis	IntechOpen	M. Jedrzejczak-Silicka and E. Mijowska	Poland	Review (book chapter)	Biocompatibility, cytotoxicity			HoloMonitor M4	Review describing and comparing various assays used to study biocompatibility and cytotoxicity of nanomaterials. Holographic phase imaging is pointed out as an excellent tool for cell morphometric characterisation and cell migration studies, and the authors conclude that the interest in the use of DH microscopy in research is constantly increasing.
01/08/2018	Direct activation of platelets by addition of CaCl2 leads coagulation of platelet-rich plasma	International Journal of Implant Dentistry	S. Toyoda et al.	Japan	Coagulation	Morphology, cell volume, roughness	Platelets	Human platelets	HoloMonitor M4	The authors have investigated the effects on coagulation by Ca2+. This may be of importance in implant dentistry, and the possibility to control platelet activation levels could be useful. By adding Ca2+ to platelets demonstrated that Ca2+ may directly activate washed platelets and promote clot formation. HoloMonitor was used to study details of platelet morphology changes and reactions to Ca2+.
19/07/2018	Interaction of Positively Charged Gold Nanoparticles with Cancer Cells Monitored by an in Situ Label-Free Optical Biosensor and Transmission Electron Microscopy	ACS Applied Materials & Iterfaces	B. Peter et al.	Hungary	Biosensors	Cell morphology	HeLa	HeLa, cervical cancer cell line	HoloMonitor M4	B. Peter with coauthors have studied nanoparticle and cell interaction in real-time. Results have shown that nanoparticle uptake is an active cell process and positively charged particles are uptaken by HeLa cells more efficiently compared to negatively charged particles. In this study, HoloMonitor M4 was used to assess cell morphology, specifically cell area.
09/07/2018	Bi-allelic loss of CDKN2A initiates melanoma invasion via BRN2 activation	Cancer Cell	H. Zeng et al.	USA	Melanoma	Cell division, cell death, senescence, motility	Melanocytes	Engineered primary human melanocytes	HoloMonitor M4	Utilizing precision genetic engineering and PHI’s HoloMonitor technology, scientists at University of California, San Francisco, have for the first time been able to monitor and map how mutations break down the genetic protection against skin cancer, allowing harmless moles to transform into invasive skin cancer.
25/06/2018	CAT3, a prodrug of 13a(S)-3-hydroxyl-6,7-dimethoxyphenanthro[9,10-b]-indolizidine, circumvents temozolomide-resistant glioblastoma via the Hedgehog signaling pathway, independently of O6-methylguanine DNA methyltransferase expression	OncoTargets and Therapy	M. Ji et al.	China	Cancer research	Cell count	U251/TMZ and T98G	TMZ-resistant glioblastoma cell lines	HoloMonitor M4	The results indicate that CAT3 is a potential therapeutic agent for TMZ-resistant GBM (glioblastoma multiforme). HoloMonitor M4 was used to directly monitor and calculate the cell number.
23/06/2018	Quantitative phase imaging for label-free analysis of cancer cells—focus on digital holographic microscopy	Appl. Sci.	Z. El-Schich et al.	Sweden and Italy	Review	QPI applied to cancer research			QPI and HoloMonitor M4	Review providing a current insight into QPI (quantitative phase imaging) applied to cancer research. The authors conclude that QPI is an ideal method for studying live cell dynamics by providing data from noninvasive monitoring over arbitrary time scales. The effect of drugs on migration, proliferation, and apoptosis of cancer cells are emerging fields suitable for QPI analysis. The authors also point out that need for QPI applications in clinical cancer diagnostics and treatments is emerging.
18/06/2018	Quantitative evaluation of morphological changes in activated platelets in vitro using digital holographic microscopy	Micron	Y. Kitamura et al.	Japan	Platelet activation	Morphology	Platelets	human platelets	HoloMonitor M4	The feasibility of DHM (Digital Holographic Microscopy) in the quantitative evaluation of morphological changes in activated platelets was evaluated. Data shown implies that DHM using the HoloMonitor could be a promissing method for quantitative examination of morphological changes in platelets in vitro. The publication also includes a comparision of the performance of different techniques for this purpose.
03/04/2018	The impact of circulating preeclampsia-associated extracellular vesicles on the migratory activity and phenotype of THP-1 monocytic cells	Scientific Reports	A. F. Kovacs et al.	Hungary	Pathogenesis in preeclampsia	Cell movements	THP-1	Monocytes	HoloMonitor M4	The study suggests that circulating extracellular vesicles (EVs) activate THP-1 monocytes, induce intra-monocytic TNF expression, as well as an altered chemotaxis and cell migration pattern. This is the first study to investigate in preeclampsia the effects of circulating EVs on monocyte chemotaxis, cell adhesion and migration in vitro. The HoloMonitor M4 was used for cell tracking of adherent cells. In the present experiments the chemokinetic effects were evaluated by measurement and calculation of migration and motility.
23/02/2018	Label-free optical biosensor for real-time monitoring the cytotoxicity of xenobiotics: A proof of principle study on glyphosate	Journal of Hazardous Materials	E. Farkas et al.	Hungary	Cytotoxicity	Viability, morphology	MC3T3-E1	Mammalian osteoblastic cell line	HoloMonitor M4	In the present work, the herbicide product Roundup Classic, its formulant (POEA) and its active ingredient (glyphosate) were studied on a mammalian osteoblastic cell line (MC3T3-E1) model using the optical biosensor Epic BT. Cell viability was monitored by HoloMonitor M4 by analyzing three-dimensional morphological structures of the cells.
23/02/2018	Applications of quantitative time lapse holographic imaging to the development of complex pharmaceutical nano formulations	Proceedings Volume 10503, Quantitative Phase Imaging IV, SPIE BiOS, 2018	E. Luther et al.	USA	Method development	Mitosis, cell death, cell-to-cell interactions, motility	tumor micro-environment		HoloMonitor M4	The authors rely on in vitro cellular cultures to evaluate the effects of the components of multifunctional nano-based formulations under development. Using HoloMonitor they efficiently obtained vectors quantifying feature changes over time in various sample conditions, i.e., changing compound concentrations or multi-compound combinations.
23/02/2018	Quantification of mammalian tumor cell state plasticity with digital holographic cytometry	Proceedings Volume 10503, Quantitative Phase Imaging IV, SPIE BiOS, 2018	M. Hejna et al.	USA	Cancer Research	Morphology	Human melanoma cell	Human melanoma cell lines of known metastatic potential	HoloMonitor M4	Working with a HoloMonitor M4 digital holographic cytometry platform, we have established a machine learning-based pipeline for high accuracy and label-free classification of adherent cells.
23/02/2018	The evolution of Phase Holographic Imaging from a research idea to publicly traded company	Proceedings Volume 10503, Quantitative Phase Imaging IV, SPIE BiOS, 2018	P. Egelberg	Sweden	Review	Label-free kinetic cell analysis			HoloMonitor M4	It is discussed how relevant biological information can be extracted from a sequence of quantitative phase images, with negligible user assistance and parameter tweaking, to simultaneously provide cell culture characteristics such as cell growth rate, viability, division rate, mitosis duration, phagocytosis rate, migration, motility and cell-cell adherence without requiring any artificial cell manipulation.
20/02/2018	Contribution of TMEM16F to pyroptotic cell death	Cell Death and Disease	J. Ousingsawat et al.	Germany	Apoptosis, pyroptosis	Cell volume	HAPI	Haploid Leukemia Cell Line	HoloMonitor M4	The role of TMEM6F in pyroptosis was investigated and the data suggests that TMEM16F has a significant impact on pyroptotic cell lysis and subsequent phagocytosis by neutrofils. HoloMonito was used to analyse cell volume changes.
18/02/2018	A time-lapse, label-free, quantitative phase imaging study of dormant and active human cancer cells	JoVe (video journal)	J. Huang, P. Guo and M.A. Moses	USA	Cancer Research	Morphology, proliferation, mitosis, migration, motility	KHOS	Human osteosarcoma cells	HoloMonitor M4	The video and publication demonstrate how HoloMonitor is used to conduct time-lapse and labeling-free characterizations of angiogenic and non-angiogenic human osteosarcoma KHOS cells. A panel of cellular parameters, including cell morphology, proliferation, and motility, were quantitatively measured and analyzed using HoloMonitor.
13/01/2018	Kynurenic acid and its derivatives are able to modulate the adhesion and locomotion of brain endothelial cells	Journal of Neural Transmission	E. Lajkó et al.	Hungary	Neurodegenerative disorders	Motility, migration, chemokinesis, morphology	GP8	Rat brain endothelial cells	HoloMonitor M4	Holomonitor M4 was used to analyse both chemokinetic responses and morphometry. The results obtained on endothelial responses and its amide derivatives.
11/01/2018	β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release	American Journal of Physiology-Lung Cellular and Molecular Physiology	Y.-W. Chang et al.	China, Japan and Taiwan	Acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS)	Area, volume, images and videos	neutrofils		HoloMonitor M4	Time-lapse videos and cell morphology images were recorded with a HoloMonitor M4 to assess the effects of the compound on neutrophil-platelet interaction and activation
15/12/2017	iTRAQ-based quantitative proteomics reveals the new evidence base for traumatic brain injury treated with targeted temperature management	Neurotherapeutics	S.-X. Cheng et al	China	Neurology	Cell area, cell volume	SH-SY5Y and HT-22	Human neuroblastoma cells (SH-SY5Y) and mouse hippocampal cells (HT-22)	HoloMonitor M4	Targeted temperature management (TTM) modulation on traumatic brain injury (TBI). HoloMonitor was used to analyze cell area and cell volume after exposure of cells to hypothermia or normothermia.
21/10/2017	Tau phosphorylation and amyloid-β deposition in the presence of formaldehyde	Formaldehyde and Cognition	J. Lu and R. He	China						Mechanisms related to Alzheimer’s disease (AD). Formaldehyde is shown to be a strong trigger of Aβ deposition and Tau phosphorylation, so that the dysmetabolism of endogenous formaldehyde should occur in the early stage of AD as formaldehyde disturbs nervous system in different pathways and in diverse manners.
13/10/2017	NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression	Nature Communications	J. Ju et al	China	human lung cancer cells	Viability, invasion	human lung cancer cells	Two independent, stable NatD knockdown human lung cancer H1299 cell lines developted by the authors	HoloMonitor M4	The authors report that NatD (N-α-acetyltransferase D) promotes the migratory and invasive capabilities of lung cancer cells in vitro and in vivo. On the ohter hand, depletion of NatD suppresses the epithelial-to-mesenchymal transition (EMT) of lung cancer cells. These findings indicate that NatD is a crucial epigenetic modulator of cell invasion during lung cancer progression.
12/10/2017	Zinc enhances the cellular energy supply to improve cell motility and restore impaired energetic metabolism in a toxic environment induced by OTA	Scientific Reports	X. Yang	China		Motility, migration	HEK293	Human embryonic kidney cells	HoloMonitor M3	Investigating the function of zinc in stimulated cell motility and motility speed in normal cell lines. Aferwards, energy metabolism pathways and impaired energetic metabolism models were introduced to show the function of zinc in the cellular energy supply.
12/10/2017	The role of vimentin in regulating cell invasive migration in dense cultures of breast carcinoma cells	Nano Letters	Y. Messica	Israel	Cell mechanics	Cell migration	MDA231 and MDA231 vim-	Highly invasive breast carcinoma cells. MDA231 vim-: knockout cells, which do not express cytoskeletal filament protein - vimetin	HoloMonitor M4	HoloMonitor M4 was used to track the migration of single cell.
04/10/2017	Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy	Cell Cycle	S. Kamlund et al.	Sweden	Cancer cell biology	Motility, migraion, proliferation, cell cycle	JIMT-1, L929, MCF-7	JIMT and MCF-7 human breast cancer cells	HoloMonitor M3 and M4	Digital holographic microscopy was used for longitudinal cell tracking of individual cells. Various parameters were investigated and the results suggest that EMT (epithelial to mesenchymal transision) and MET (mesenchymal to epithelial transission) can be investigated using this method.
01/10/2017	A biophysical approach to quantify skeletal stem cells trans-differentiation as a model for the study of osteoporosis	Biophysical Chemistry	L. Petecchia et al.	Italy	skeletal stem cell differentiation, osteoporosis	Morphology	hBM-MSCs	Human bone marrow-derived mesenchymal stem cells	HoloMonitor M3	Digital Holography allowed to extract a set of quantitative indicators associated with the specific biological state and, among all possible parameters, the average thickness, T, and the roughness kurtosis, RK, allowed to produce a scatter plot in which full osteoblasts, full adipocytes and undifferentiated hBM-MSC lie in well separated regions of the space. Noteworthy, this T-RK graph was successfully extended to all conditions and provided a clear and prompt visualization of the cellular commitment in terms of position in the T-RK plane
26/09/2017	Interleukin-6 blockade attenuates lung cancer tissue construction integrated by cancer stem cells	Scientific Reports	H. Ogawa et al.	Japan	Lung cancer	Motility, migration	human lung cancer cells	lung cancer stem cell-like cells from human lung cancer cell line	HoloMonitor M4	The authors established technologies to generate lung CSC (cancer stem cell) -like cells from human lung cancer cell line A549 by introducing OCT3/4, SOX2 and KLF4, and to construct “lung cancer organoids” in vitro that mimicked human lung cancer tissues. Through the use of these technologies and the evaluation of clinical samples, interleukin-6 was itentified as a novel potential therapeutic target for lung cancer stem cells.
21/09/2017	Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi	Nature Communications	I. Yeh et al.	USA	Melanoma development	Cell volume	melana-a	Stably tranduced melanocytes	HoloMonitor M4	HoloMonitor was used to measure cell volume. Together with other methods the results identify DPN (deep penetrating nevus) as an intermediate melanocytic neoplasm, with a progression stage positioned between benign nevus and DPN-like melanoma.
20/09/2017	High accuracy label-free classifcation of single-cell kinetic states from holographic cytometry of human melanoma cells	Scientific Reports	M. Hejna et al.	USA	Melanoma	Panel of 42 parameters	A375	Human melanoma cell line	HoloMonitor M4	The authors used machine learning to develop a method for robust and kinetic label-free classification of single adherent cells info functional states.
15/09/2017	Dynamics of morphological changes of adherent cultured cells at a range of low temperatures near physiological temperature (time-lapse imaging by digital holographic microscopy)	Cryobiology and Cryotechnology	H. Ishiguro, M. Uemura and K. Nagasaki	Japan	Thermal stress	Morphology, motility, cell growth, cell division	PC3	Human prostate carcinoma cell line	HoloMonitor M3	This study investigated the dynamics of morphological changes, including mobility of adherent cultured cells, under low-temperature stress near physiological temperature, using time-lapse three-dimensional imaging with digital holographic microscopy (DHM).
15/09/2017	Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation	Experimental Cell Research	L. Petecchia, C. Usai, M. Vassalli and P. Gavazzo	Italy	Biocompatibility and osteointegration potential of clinical titanium implants	Morphology (area, perimeter, volume, thickness, irregularity)	(hBM-MSC)	Mesenchymal stem cells from human bone marrow	HoloMonitor M3	The aim of the study was to evaluate the effectiveness of a nano-patterned titanium surface in sustaining hBM-MSC adhesion, growth and differentiation.
05/09/2017	CFTR supports cell death through ROS-dependent activation of TMEM16F (anoctamin 6)	Eur J Physiol	F.Simões et al.	Germany	Cystic fibrosis	Morphology, cell volume	HEK293, HeLa, and CFBE cells	Human embryonic kidney cells (HEK293), human cervical cancer ( HeLa), and cystic fibrosis bronchial epithelial (CFBE) cells	HoloMonitor	Mechanism in cystic fibrosis; role of induced cell death by reactive oxygen species (ROS) was investigated. Cell volume measurements using HoloMonitor was used to assess cell shrinkage during cell death.
01/07/2017	Standardized polyalthia longifolia leaf extract (PLME) inhibits cell proliferation and promotes apoptosis: the anti-cancer study with various microscopy methods	Biomedicine & Pharmacotherapy	S. Vijayarathnaa et al.	Malaysia, Australia	Apoptosis, imaging technology, cytotoxicity	Morphology	HeLa, Vero	HeLa:Human cervical cancer; Vero: green monkey kidney epithelial cells (non-cancerous cells)	HoloMonitor M3	In this study, the apoptogenic property and the cytotoxic effect of P. longifolia leaf methanolic extract (PLME) against the human cervical carcinoma cells (HeLa) was studied using light microscope (LM), digital holographic digital microscopy (DHM), scanning electron microscope (SEM) and transmission electron microscope (TEM).
26/06/2017	Synthesis, characterization and in vitro and in vivo photodynamic activities of a gallium(III) tris(ethoxycarbonyl)corrole	Dalton Transactions 2017	Z. Zhang et al.	China	Photo-cytotoxicity; photodynamic therapy (PDT) for cancer cells.	Morphology (area, thickness, shape), apoptosis	A549	Human lung carcinoma cells	HoloMonitor M3	After illumination and treatment with 1-Ga, A549 cells exhibited characteristics typical of apoptosis, spherical shape of the cells, chromatin condensation, and nuclear membrane irregularity. After incubation for 1 h, the authors found that the cells started to contract, the area decreased and the thickness increased.
19/06/2017	Accumulation of simulated pathological level of formaldehyde decreases cell viability and adhesive morphology in neuronal cells	Progress in Biochemistry and Biophysics	W. Jing et al.	China	Age-related cognitive impairment	Morphology; thickness, area and volume	N2a cells	Murine neuroblastoma	HoloMonitor M3	Chronic formaldehyde (FA) exposure leads to impairment of human memory. Using HoloMonitor Wang Jing and coworkers illustrate how FA exposure influence the cell morphology in terms of thickness, area and volume.
19/06/2017	Hypoxia-inducible factor-1 alpha is involved in RIP-induced necroptosis caused by in vitro and in vivo ischemic brain injury	Scientific reports	X.-S. Yang et al.	China	Ischemic brain injury, necroptosis	Cell count (used for viability)	HT-22	Murine hippocampal neuronal	HoloMonitor M4	These findings indicate that necroptosis contributes to ischemic brain injury induced by OGD and MCAO and implicate HIF-1α, RIP1, RIP3, and MLKL in necroptosis.
05/06/2017	Ouabain suppresses the growth and migration abilities of glioma U‑87MG cells through inhibiting the Akt/mTOR signaling pathway and downregulating the expression of HIF‑1α	Molecular Medicine Reports	X.‑S. Yang et al.	China	Cancer Research	Cell count, cell motility, 3D cell culture	U87MG cells	Human Glioblastoma	HoloMonitor M4	The present study revealed that ouabain intervention inhibited U‑87MG cell viability and cell motility in a dose‑dependent manner, suggesting that ouabain promoted the apoptosis of U87‑MG cells. HoloMonitor was used to analyse cell motility, cell count and to study the 3D cell structure.
26/05/2017	Mannich curcuminoids as potent anticancer agents	Archiv der Pharmazie	M. Gyuris et al.	Hungary	drug activity	Area, thickness, roughness, volume, eccentricity, irregularity	A549 and H1975	lung adenocarcinoma cells	HoloMonitor M3	Structure–activity relationship (SAR) studies were performed by applying viability assays and holographic microscopic imaging to these curcumin analogues for anti-proliferative activity against A549 and H1975 lung adenocarcinoma cells. The TNFα-induced NF-κB inhibition and autophagy induction effects correlated strongly with the cytotoxic potential of the analogues. Significant inhibition of tumour growth was observed when the most potent analogue 44 was added in liposomes at one-sixth of the maximally tolerated dose in the A549 xenograft model.
10/05/2017	History of cell culture	Biochemistry, Genetics and Molecular Biology » "New Insights into Cell Culture Technology"	M. Jedrzejczak-Silicka	Poland	Cell biology	Various	various	various	HoloMonitor	Book chapter review
02/05/2017	Hydroxylated fluorescent dyes for live-cell labeling: synthesis, spectra and super-resolution STED	Chemistry — A European Journal	A. N.Butkevich et al.	Germany	Method development	Cell quality, morphology, growth	Drosophilia S2 cells		HoloMonitor M4	Cell viability in the presence of dyes in the centromeres was verified by measuring cell morphology of Drosophila S2 cells using a holographic microscope (Holomonitor M4). Images and measures cells without using invasive dyes or reagents. Using HoloMonitor, the authors proved that cells stay alive and continue to divide after staining them with the improved super-resolution dyes.
01/05/2017	Applications of label-free, quantitative phase holographic imaging cytometry to the development of multi-specific nanoscale pharmaceutical formulations	Cytometry Part A 2017	E Luther et al	USA	Review	Review; various parameters, neois	HeLa, L929	HeLa:Human cervical cancer; L929: mouse fibroblast	HoloMonitor M4	A review of HoloMonitor applications: tracking of Giant HeLa cells, which may be undergoing neosis tracking the effects of cell cycle related toxic agents on cell lines; using MicroRNAs to reverse the polarization state in macrophages to induce tumor cell killing development of liposomal nanoformulations to overcome multi-drug resistance in ovarian cancer cells and development of dual sensitive micelles to specifically target matrix metalloproteinase 2
01/05/2017	Label-free high temporal resolution assessment of cell proliferation using digital holographic microscopy	Cytometry Part A 2017	B. Janicke et al.	Sweden	develop digital holography application, cell proliferation	Cell count, confluence, volume	L929	Mouse fibroblasts	HoloMonitor M4	The authors have developed a robust and label-free kinetic cell proliferation assay with high temporal resolution for adherent cells using HoloMonitor M4. Only two image processing settings were adjusted between cell lines, making the assay practical, user friendly, and free of user bias. In the recorded time-lapse image sequences, individual cells were automatically identified to provide detailed growth curves and growth rate data of cell number, confluence, and average cell volume. The results demonstrate how these parameters facilitate a deeper understanding of cell processes than what is achievable with current single-parameter and end-point methods.
01/05/2017	Lipid deregulation in UV irradiated skin cells: role of 25-hydroxycholesterol in keratinocyte differentiation during photoaging	The journal of steroid biochemistry and molecular biology	E. Olivier et. al.	France	Ageing of skin cells	Morphology; thickness, roughness, volume, area	HaCaT-cells	human keratinocytes	HoloMonitor M3	Cell morphological changes in ageing: Skin photoaging due to UV irradiation is a degenerative process that appears more and more as a growing concern. Lipids, including oxysterols, are involved in degenerative processes; as skin cells contain various lipids, the aim of our study was to evaluate first, changes in keratinocyte lipid levels induced by UV exposure and second, cellular effects of oxysterols in cell morphology and several hallmarks of keratinocyte differentiation. Using HoloMonitor and confocal microscopy analysis, the authors highlighted cell thickening and cytoskeletal damage after incubation of keratinocytes with 25-hydroxycholesterol.
18/04/2017	Characterization of a transgenic mouse model exhibiting spontaneous lung adenocarcinomas with a metastatic phenotype	PLOS ONE	H.-W. Chang et al.	Taiwan	phenotypic and genotypoc identification of lung cancer	Motility, area	Tg-3m and Tg-6m	Primary mouse lung adenocarcinoma	HoloMonitor M4	The authors have developed a mouse model which is very similar to human, and which can be used for more relevant drug evaluations. Focus are is tumor metatasis and especially the phenomenon of the epithelial-mesenchymal transition (EMT). HoloMonitor was used to investigate the capability of cell movement of tumor cells at different stages.
12/04/2017	The “inherent vice” in the anti-angiogenic theory may cause the highly metastatic cancer to spread more aggressively	Scientific Reports	D. Wang et al.	China	Tumour metastasis	Motility, migration	Primary 4T1 tumor	mouse mammary cancer cells	HoloMonitor M4	Even if the blood supply is cut off in tumors, cells will survive, and even migrate, and maybe cause increased metastasis.
06/04/2017	Quantitative phase-contrast imaging – a potential tool for future cancer diagnostics	Cytometry Part A	A. Gjörloff-Wingren	Sweden	Review	Review; various parameters	N/A	N/A	review	Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures
01/04/2017	In situ morphological assessment of apoptosis induced by Phaleria macrocarpa (Boerl.) fruit ethyl acetate fraction (PMEAF) in MDA-MB-231 cells by microscopy observation	Biomedicine & Pharmacotherapy	N. Kavitha et al.	Malaysia	assessment of apoptosis	Area, thickness	MDA-MB-231	MDA-MB-231: Human mammary carcinoma cell lines; Vero: green monkey kidney epithelial cells (non-cancerous cells)	HoloMonitor M3	The purpose of the study was to determine the cytotoxic effect of P. macrocarpa fruit ethyl acetate fraction (PMEAF) by using various conventional and modern microscopy techniques. The results indicate that PMEAF is a potential anticancer agent and a candidate for developing more potent anticancer drugs.
30/03/2017	Promising anticancer activity of cyclotrichium niveum l. extracts through induction of both apoptosis and necrosis	Food and Chemical Toxicology	A. Özdemir et al.	Turkey	Cytotoxic effects of plant extracts	Cell area, cell volume and average thickness	HeLa, MCF-7 cancer cells, and MRC-5 and MCF-10A normal cells	HeLa:Human cervical cancer; MCF-7: human breastcancer; MRC-5: Human embryo lung fibroblasts; MCF10A: normal-like human breast cells	HoloMonitor M4	Aim was to study the impact of plant extracts from Cyclotrichium niveumon on normal-like and cancer cell lines. The authors concluded that C. niveum has cytotoxic properties in the cancer cell lines tested.
27/03/2017	Characterization of dormant and active human cancer cells by quantitative phase imaging	Cytometry Part A	P. Guo, J. Huang and M. A. Moses	USA	angiogenesis	Area, thickness, migration, motility	KHOS	human osteosarcoma	HoloMonitor M4	Based on HoloMonitor M4, the authors developed a novel in vitro method to characterize the angiogenic and non-angiogenic phenotypes of human osteosarcoma cells. Compared with traditional in vitro assays, the approach provides a non-invasive and low-cost method to assess a panel of cell morphology and cell behaviors. The authors were able to easily and accurately differentiate between the angiogenic and non-angiogenic phenotypes of KHOS cells by comparing five cellular parameters: cell area, cell thickness, cell migration, cell motility and cell motility speed.
22/03/2017	Holography: the usefulness of digital holographic microscopy for clinical diagnostics	Holographic Materials and Optical Systems 2017	El-Schich	Sweden	Book chapter, review	Cell death, cell cycle, area, volume	U2932, WM-266-4, CHL-1	Large B-cell lymphoma (U2932), human melanoma (WM-266-4 and CHL-1)	HoloMonitor M4	Open access book chapter discussing the potential of holographic microscopy in clinical applications
07/03/2017	Loss of ERα induces amoeboid-like migration of breast cancer cells by downregulating vinculin	Nature Communications	Y. Gao et al.	China	cancer cell movement	Motility	MCF-7	Human breast cancer cultured in matrigel	HoloMonitor M4	The authors tracked and analyzed the migration of different MCF-7 cell populations in a 3D matrix. In addition to measuring the cell migration speed with HoloMonitor, the following five phenotypes were observed: ‘stable adhesion’, ‘unstable lamellipodia’, ‘bleb’, ‘stable bleb’ and ‘unstable pseudopod’.
01/03/2017	Three-dimensional morphology of C6/36 cells infected by dengue virus: a study based on digital holographic microscopy	Journal of Southern Medical University	Y, JH, et al.	China	Virus infection	Area, thickness, volume	C6/36 cells	Larvae	HoloMonitor M4	Digital holographic microscopy allows monitoring of the complex morphological changes of cells during DENV infection. The 4 DENV strains with different serotypes causes characteristic cell damages during infection
20/02/2017	Supervised classification of etoposide-treated in vitro adherent cells based on noninvasive imaging morphology	Journal of Medical Imaging	A. Leida Mölder et al.	Sweden	Image-based analysis, cell classification	Morphology	DU-145	Prostate cancer cells	HoloMonitor M4	The morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we report a best case sensitivity of 88% and specificity of 94% for classification of cells as treated/untreated.
02/11/2016	Cathelicidins positively regulate pancreatic β-cell functions	The faseb journal	J. Sun et al.	China, Sweden, France	Immunregulation	Cell density	INS-1, INS-1 832/13	INS-1 832/13, a modified rat insulinoma INS-1 cell clone stably transfected with the human proinsulin gene,	HoloMonitor M4	HoloMonitor M4 was used to assess the cell density during cell proliferation.
12/10/2016	Non-invasive, quantitative assessment of the morphology of γ-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy	International journal of radiation biology	T. Kawase	Japan	Stem cell therapy	Cell morphology	MSC-AT, MSC-BM	Mesenchymal stem cells derived from adipose tissue (MSC-AT) and bone marrow (MSC-BM),	HoloMonitor M4	Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.
01/10/2016	Reversal of chemoresistance in ovarian cancer by co-delivery of a p-glycoprotein inhibitor and paclitaxel in a liposomal platform	Molecular Cancer Therapeutics	Y. Zhang	USA	Cancer	Cell cycle arrest, apoptosis	SKOV 3 TR cells	Ovarian cancer cell line	HoloMonitor M4	The authors created a nanoparticle formulation against chemotherapy resistant ovarian cancer, loaded with the commonly used cancer drug paclitaxel together with a drug that breaks down the resistance mechanism of cancer cells. Using HoloMonitor, the scientists were able to show that paclitaxel-resistant ovarian cancer cells stopped multiplying when treated with the nanoparticles.
30/09/2016	Hepatitis B virus X induces inflammation and cancer in mice liver through dysregulation of cytoskeletal remodeling and lipid metabolism	Oncotarget	Z. Xu et al	China	Cancer	Cell migration	Huh-7	Huh-7 - human liver cell line	HoloMonitor M4	In this study, Z. Xu with colleagues have defined the pathological mechanism for the occurrence and development of Hepatitis B virus X protein induced liver cancer. Authors have employed HoloMonitor M4 to study the changes of single cel mimigration after CDC42 gene knowkdown in Huh-7 cells.
17/08/2016	Ca2+ signals, cell membrane disintegration, and activation of TMEM16F during necroptosis	Cellular and molecular life sciences	J. Ousingsawat	Germany	Cell death (necroptosis)	Volume, morphology kinetics	NIH3T3, HT29	NIH3T3:Mouse embryonic fibroblasts; HT29:Human colonic carcinoma	HoloMonitor M4	It is well known that cells swell during necroptosis. However, proper cell volume measurements are still missing. Using HoloMonitor M4 the authors non-invasively measured the cell volume changes associated with necroptosis. The results were confirmed by flow cytometry measurements.
12/08/2016	Relationship between TMEM16A/anoctamin 1 and LRRC8A	European journal of physiology	R. Benedetto	Germany/Portugal	Basic cell research	Changes in cell volume, hight and diameter (swelling and shrinking)	HEK 293	Human embryonic kidney cells	HoloMonitor M4?	Analysis of hypo-induced changes in cell height rather than cell volume, as an increase in cell height is not compromised by basal adhesions or cell/cell contacts. Cell height measured by holographic microscopy in HEK293 cells, indicated hypotonic cell swelling (Hypo) within seconds and completed regulatory volume decrease (RVD) within less than 2 min
19/07/2016	Mixed nanosized polymeric micelles as promoter of doxorubicin and miRNA-34a co-delivery triggered by dual stimuli in tumor tissue	Small	G. Salzano et al.	USA	drug delivery	Motility, migration, volume, thickness, area	HT1080	Human fibrosarcoma	HoloMonitor M4	Methods for producing 4D plot were developed. Images from the time course were exported to ImageJ and treated as an image stack, creating a volume rendering of the cellular motion over time of analysis. The brightness threshold was adjusted to view all cells within the imaging area or only the thicker cells, a feature that allows identification of mitosis, apoptosis, and transition between mesenchymal and amoeboid cellular morphologies
16/06/2016	Biophysical characteristics of proteins and living cells exposed to the green tea polyphenol epigallocatechin-3-gallate	European biophysics journal	Beatrix Peter, Szilvia Bosze and Robert Horvath	Hungary	Review. Mode of action	Various; cell adhesion, motility, migration, stiffness, apoptosis and proliferation				HoloMonitor-based methods and other methods to assess cell adhesion, motility, migration, stiffness, apoptosis and proliferation exposed to green tea polyphenol epigallocatechin-3-gallate are reviewed.
13/05/2016	Label-free optical biosensors for monitoring cellular processes and cytotoxic agents at interfaces using guided modes and advanced phase-contrast imaging techniques	Advanced sciences and technologies for security applications	I. Székács, R. Horvath and A. Székács	Hungary	Review	Review
18/04/2016	Ion channels in regulated cell death	Cellular and molecular life sciences	K. Kunzelmann	Germany	Cell death (review)	Cell death				Despite the rather different morphologic appearances of apoptosis and necroptosis, both mechanisms of cell death require the transport of ions, organic osmolytes, and water. The contribution of these transport proteins and underlying mechanisms are summarized in this review.
31/03/2016	Shielding of the geomagnetic field alters actin assembly and inhibits cell motility in human neuroblastoma cells	Scientific reports	W-C. Mo et al.	China, Australia	Mode of action	Volume, thickness, area, perimeter length, irregularity, eccentricity, motility	SHSY-5Y	Human neuroblastoma	HoloMonitor M4	Using holomonitor M4, the authors show that the elimination of Earth’s magnetic field affects cell behavior. Potential influence on cancer treatments suggested.
24/03/2016	Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine lactone elicits changes in cell volume, morphology, and AQP9 characteristics in macrophages	Frontiers in cellular and infection microbiology	A. Holm, K-E. Magnusson and E. Vikström	Sweden	Mode of action; bacterial influence on macrophages	Cell volume	Human primary macrophages		HoloMonitor M4	Analysis of optical cell volume changes; time-lapse videos were recorded using the holomonitor M4 Image acquisition was set at an interval of 30 s for 1 h.
04/03/2016	The curcumin analog C-150, influencing NF-κB, UPR and Akt/Notch pathways has potent anticancer activity in vitro and in vivo	Plos one	L. Hackler	Hungary	Cancer	Cell morphology (volume, area, thickness)	U87 MG, U-251 MG, GBM1, GBM2	human glioma cell lines	HoloMonitor M3	Visualization of cytotoxic effects using holomonitor. These experiments record morphological parameters of treated cells such as area, thickness and volume. First time where the effects of drug induced cell death on cellular morphology of these glioma cells were detected by using 3D holographic images.
31/12/2015	Incubator proof miniaturized HoloMonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections	J. Biomed. Opt	B. Peter et al.	Hungary	Basic research, nanostructures	Motility, migration, cell adhesion	HeLa, MC3T3-E1	HeLa: human cervical cancer; MC3T3-E1 mouse osteoblastic cell line	Holomonitor M4	Holomonitor M4 was used to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications.
24/12/2015	Enhanced cytotoxicity of folic acid-targeted liposomes co-loaded with C6 ceramide and doxorubicin: in vitro evaluation on HeLa, A2780-ADR and H69-AR cells	Molecular pharmaceutics	S. Kumar Sriraman et al.	USA	Cancer, Drug efficacy	Aera, apoptosis	HeLa	Human cervical cancer	HoloMonitor M4	Using holomonitor M4, it was found that FA-(C6+Dox)-LP treated HeLa cells underwent rapid progression to apoptosis (cell suicide) after 21 hours, as evidenced by a drastic drop in cell area after loss of cell membrane integrity.
22/12/2015	From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behavior	Scientific reports	H. Persson et al.	Sweden	Basic research	Cell migration, division, morphology	L929	Mouse fibroblasts	HoloMonitor M3	Understanding of the interactions between cells and nanowires. The results provide guidelines to minimize cellular stress on nanowire arrays. Additionally, the findings show that cell behavior can be controlled by adjusting nanowire density, which may have applications in drug development.
18/12/2015	Targeted tumor therapy by rubia tinctorum l.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines	Cancer cell international	E. Lajkó et al.	Hungary	Cancer, drug efficacy	Migration, migration directness, motility and motility speed	A2058, HT168-M1, MRC-5	Human melanoma cell lines (A2058, HT168-M1) Human fibroblast cells (MRC-5)	HoloMonitor M4	Our results highlight the effective and selective inhibitory activity of purpurin towards melanoma cells and its possible use as a targeted anticancer agent. The anthraquinones of the cytotoxic extract are suggested to apply in drug delivery systems as an anticancer drug
30/11/2015	Poloxamer bioadhesive hydrogel for buccal drug delivery: cytotoxicity and trans-epithelial permeability evaluations using TR146 human buccal epithelial cell line	International journal of pharmaceutics	N. Zeng et al.	France	Drug delivery	Viability, membrane integrity, apoptosis	Tr146	human buccal epithelial cell line	Holomonitor M3	The assessment of cell viability (MTT, Alamar Blue), membrane integrity (Neutral Red), and apoptosis assay (Hoechst 33342), were performed and associated to holomonitor M3 analysis.
09/06/2015	Reversal of chemoresistance in ovarian cancer by co-delivery of mdr inhibitors and paclitaxel using a targeted liposomal platform	The faseb journal	S. Kumar Sriraman et al.	USA	Cancer, chemoresistance	Mitotis length, apoptosis	SKOV3	human ovarian cancer line	HoloMonitor M4	Using time-course holographic imaging studies, this cell cycle arrest followed by progression to apoptosis was clearly visualized and confirmed.
30/04/2015	Macrophage polarization and the effect of microRNA-155 administered in water-in-oil-in-water multiple emulsion formulations	Journal of Clinical & Cellular Immunology	A. Oka et al.	USA	Cancer/immunoncology, macrophage polarization	Morphology motility, apoptosis, coculture	J774A.1, SKOV3	MJ774A.1 murine macrophage cell line; SKOV3 human ovarian cancer cells	Holomonitor M4	Holographic assessment using Holomonitor M4 of the co-cultured cells in real-time showed differences in motility and morphology of macrophages with mir-155 ME treated cells showing greater cellular interaction between the two phenotypes.
20/03/2015	Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume	Future science oa	Z. El-Schich et al.	Sweden	Cancer, drug delivery, microarray	Cell number, mean area, thickness, volume	Jurkat, U2932	Jurkat: human T-cell acute lymphoblastic leukemia; U2932: human diffuse large B-cell lymphoma	HoloMonitor M2	The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.
01/03/2015	Induction of morphological changes in death-induced cancer cells monitored by holographic microscopy	Journal of structural biology	Z. El-Schich et al.	Sweden	Cell death morphology	Confluence, cell number, area, volume	L929, DU145	L929: mouse fibroblast; DU145: human prostate cancer	HoloMonitor M3	Using HoloMonitor M3, the authors show that holographic microscopy allows label-free and completely non-invasive morphological measurements of cell growth, viability and death.
01/12/2014	Environmental and toxicological impacts of glyphosate with its formulating adjuvant	International Journal of Agricultural, Biosystems Science and Engineering	I. Székács et al.	Hungary	Effect of drug	Area, thickness	NE-4C	mouse embryonic neuroectodermal	Holomonitor M4	The article describes a study of the health effects of the well-known, widely used herbicide Roundup. In the study, the HoloMonitor M4 was used to assess toxic effects to animal cells after exposure to Roundup.
10/09/2014	Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in L929 cells	PLOS ONE	M. Falck Miniotis et al.	Sweden	Method development	Cell volume to study cell cycle arrest	L929	mouse fibroblast	Holomonitor M3	We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.
02/09/2014	Combination of unsaturated fatty acids and ionizing radiation on human glioma cells: cellular, biochemical and gene expression analysis	Lipids in health and disease	O. Antal et al.	Hungary	Increase cancer treatment efficiency	Cell number, confluence, irregularity, thickness	U87MG	human glioma cells	HoloMonitor M3	Holomonitor M3 was used to assess the increased death of glioma (brain tumor) cells when combining irradiation with various unsaturated fatty acid treatments
28/07/2014	The role of PI3K/AKT-related PIP5K1α and the discovery of its selective inhibitor for treatment of advanced prostate cancer	PNAS	J. Semenas et al.	Sweden, Finland	Mode of action, prostate Cancer	Morphology, cell size	PC-3	human prostate cancer cells	holomonitor M3	The HoloMonitor M3 imaging system was applied to capture living PC-3 cells in culture during treatment
17/06/2014	Edaravone protects against methylglyoxal-induced barrier damage in human brain endothelial cells	PLOS ONE	A. E. Tóth et al.	Hungary, France, Japan	Mode of action. Drug against brain damage after acute brain ischemia and infarction	Area, thickness	hCMEC/D3	Human brain endothelial cells	Holomonitor M3	Using holographic microscopy for label-free analysis of living cells, visualized for the first time the kinetics of morphological changes caused by methylglyoxal. The morphology data confirmed that edavarone completely prevented methylglyoxal-induced changes in morphology and detachment of cells.
27/06/2013	Fibroblasts cultured on nanowires exhibit low motility, impaired cell division, and DNA damage	Small	H. Persson et al.	Sweden	Cells on nanomaterials	Motility	L929	Mouse fibroblasts	HoloMonitor M4	Fibroblast behaviour on vertical nanowire arrays was investigated using the HoloMonitor M3. It was shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration.
29/05/2013	Cells and holograms — holograms and digital holographic microscopy as a tool to study the morphology of living Cells	Holography — Basic Principles and Contemporary Applications	K. Alm et al.	Sweden	Review	Review			DHM
29/04/2013	Lipids as tumoricidal components of human α-lactalbumin made lethal to tumor cells (HAMLET): unique and shared effects on signaling and death	J. Of Biological Chemistry	J. C. S. Ho et al.	Sweden	Action of HAMLET	Cell morphology	A549	human lung carcinoma cells	Holomonitor M3	The authors used the holomonitor M3 to observe morphological differences on cells over time, treated with either HAMLET or various lipids associated with HAMLET.
17/03/2013	A unifying mechanism for cancer cell death through ion channel activation by HAMLET	PLOS One	P. Storm et al.	Sweden	Action of HAMLET	Cell morphology			Holomonitor M2	Changes in cell morphology was studied using HoloMonitor technology
24/01/2013	Prevention and treatment of colon cancer by ceroral administration of HAMLET	Gut	M. Puthia et al.	Sweden	Action of HAMLET	Thickness, area	DLD1	human colon cancer	Holomonitor M2	Using the HoloMonitor M2, the authors show that cell thickness and adherent surface area change dramatically after HAMLET treatment. HAMLET is a human α-lactalbumin made lethal to tumor cells.
27/12/2012	Low resolution solution structure of HAMLET and the importance of its alpha-domains in tumoricidal activity	PLOS ONE	J. C. S. Ho et al.	Sweden	Action of HAMLET	Images	A549	human lung carcinoma cells	HoloMonitor M3	Peptides covering the alpha1 and alpha2 domains of the protein triggered rapid ion fluxes in the presence of sodium oleate and were internalized by tumor cells, causing rapid and sustained changes in cell morphology. The alpha peptide-oleate bound forms also triggered tumor cell death with comparable efficiency as HAMLET. In addition, shorter peptides corresponding to those domains are biologically active.
06/11/2012	HAMLET: functional properties and therapeutic potential	Future Medicine	J. C. S. Ho et al.	Sweden	Action of HAMLET, review	Images
20/03/2012	Dermatan sulfate is involved in the tumorigenic properties of esophagus cquamos cell carcinoma	Cancer Research	M. A. Thelin et al.	Sweden	Tumorogenic properties	Area	TE1	human squamous cell carcinoma of the esophagus	N/A	Chondroitin and dermatan sulfate proteoglycans, which occur both in the matrix and at the cell surface, play important roles in thetumorgenic processes. The unique feature that distinguishes DS from CS is the presence of iduronic acid (IdoA) in DS. IdoA in DS influences tumorigenesis by affecting cancer cell behavior
08/03/2011	HAMLET binding to α-actinin facilitates tumor cell detachment	PLOS ONE	M. Trulsson et al.	Sweden	Action of HAMLET	Area, thickness	A549	lung cancer	HoloMonitor M2	Interaction between HAMLET and a-actinins promotes tumor cell detachment.
28/02/2011	Digital holography and cell studies	Holography-Research and technologies	K. Alm et al.	Sweden	review	Review
23/11/2010	Generation of trisomies in cancer cells by multipolar mitosis and incomplete cytokinesis	PNAS	D. Gisselsson Nord et al.	Sweden	Genetic defects in kidney cancer	Cell division	WiT-49	Derived from a primary lung metastasis of an aggressive Wilms kidney tumor	HoloMonitor M2	Gisselsson and colleagues studied cell division in short-term cultures established from fibroblasts and primary tumors. The researchers showed that abnormal cell divisions can cause an abnormal number of chromosomes in cancer cells.
01/11/2010	Reduction of the putative CD44+CD24 breast cancer stem cell population by targeting the polyamine metabolic pathway with PG11047	Anticancer Drugs	H. Cirenajwis et al.	Sweden	Action of polyamine inhibitors	Motility	JIMT-1	human breast cancer	HoloMonitor M2	Cirenajwis and colleagues used the label-free live cell imaging capabilities of the HoloMonitor to study the movement of JIMT-1 breast cancer cells. They showed that treatment with the ornithine analogue difluoromethylor-nithine (DFMO) increased cell motility, while treatment with the polyamine analogue PG-11047 decreased cell motility.
12/07/2010	Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: implications for specific immunotherapy	Vaccine	S. Broos et al.	Sweden	Immunotherapy, drug uptake, nanoparticle	Morphology, images	MoDC	monocyte-derived dendritic cell	HoloMonitor M2
01/06/2010	Digital holographic microscopy — innovative and non-destructive analysis of living cells	Microscopy: Science, Technology, Applications and Education	Z. El-Schish et al.	Sweden	Review	Review
01/01/2010	Cell motility studies using digital holographic microscopy	Microscopy: Science, Technology, Applications and Education	J. Persson et al.	Sweden	Develop digital holography application, motility	Motility, morphology	L929, L56Br-C1, MDA-MB-231	L929: mouse fibroblast; L56Br-C1: human breast cancer lymph node metastasis; MDA-MB-231: human epithelial mammary gland cell line	HoloMonitor M2	Using digital holography in combination with our program Celltrack, we can follow a population of cells, and we can study single cells within that population. The results also show that we can correlate cell movement with the level of confluence in the cultures, cell area, cell optical thickness and cell volume. In all, digital holography proved suitable for a cell movement assay.
31/10/2008	Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography	Journal of Microscopy	A. Mölder et al.	Sweden	Develop digital holography application	Cell counting, confluence, phase shift, optical path length	MCF-10 A, L929, PC-3,DU-145	MCF-10 A: human breast epithelia cells; L929: mouse fibroblasts; PC-3: human prostate cancer; DU-145: human prostate cancer	HoloMonitor 2	Using a unique non-invasive labelfree cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HolomonitorTM M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.
01/09/2008	A hardware acceleration platform for digital holographic imaging	Journal of VLSI Signal Processing Systems	T. Lenart, M. Gustafsson, V. Öwall	Sweden	Background technology
01/01/2008	Modeling and exploration of a reconfigurable architecture for digital holographic Imaging	IEEE	T. Lenart, H. Svensson and V. Öwall	Sweden	Background technology
01/01/2005	Object characterization with refractometric digital Fourier holography	Optics Letters	M. Sebesta and M. Gustafsson	Sweden	Background technology
01/03/2004	High-resolution digital transmission microscopy — a Fourier holography approach	Optics and Lasers in Engineering	M. Gustafsson et al.	Sweden	Background technology
01/01/2004	Refractometry of microscopic objects with digital holography	Applied Optics	M. Gustafsson and M. Sebesta	Sweden	Background technology
		Journal	Author(s)			Applications	Cell line(s)

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