Wound Healing and cell migration can be defined as the movement of cells from one location to another in response to biological/chemical signals or environmental events. Wound healing and cell migration can contribute to pathological conditions in many disease areas like immune responses, tumor metastasis and angiogenesis.
Use of Wound Healing Assays to Study Cell Migration
Wound healing assays mimic in vivo cell migration. They are in vitro assays widely used in all kinds of research related to directed cell migration.
Cell and User-friendly Wound Healing Assay
The HoloMonitor® Wound healing Assay is a classical wound healing, or scratch assay, optimized to easily provide kinetic, repetitive and semi-automatic result presentation of the gap closure and cell front velocity.
Gap closure decreases over time as the cells fill the gap. Untreated cells are used and the gap is created using the ibidi® Culture-Insert 2 Well.
- PHI HoloMonitor M4 with Hstudio software version 2.7.3 or later
- Culture vessel from ibidi®: Culture-Insert 2 Well in ibidi μ-Plate 24 Well (cat. # 80241) or Culture-Insert 2 Well in ibidi μ-Dish 35 mm, high (cat. # 81176)
- PHI vessel holder for selected ibidi® vessel
- PHI HoloLid for selected ibidi® vessel