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Wound Healing Assay

An introduction to HoloMonitor Wound Healing Assay based on live-cell imaging

Wound Healing Assay, or Scratch Wound Healing Assay, is used to study the coordinated movements of cells and to measure cell migration across a wound field, or gap. HoloMonitor Wound Healing Assay applies a label-free technology to monitor cells in the incubator.

Wound healing and cell migration can be defined as the movement of cells from one location to another in response to biological/chemical signals or environmental events. Wound healing and cell migration can contribute to pathological conditions in many disease areas like immune responses, tumor metastasis and angiogenesis.

Use of  Wound Healing Assays to Study Cell Migration

The Wound Healing assay is a standard in vivo method for cell migration. They are in vitro assays widely used in all kinds of research related to directed cell migration.

Cell and User-friendly Wound Healing Assay

The HoloMonitor® Wound Healing Assay is a classical wound healing, or scratch assay, optimized to easily provide kinetic, repetitive and semi-automatic result presentation of the gap closure and cell front velocity.

The Wound Healing Assay provides kinetic gap closure analysis showing how gap width and cell covered area change over time.

The Wound Healing Assay provides kinetic gap closure analysis showing how gap width and cell covered area change over time.

HoloMonitor® Wound Healing Assay - Get the Whole Picture

The assay is based on a cell-friendly method to create the gap and easily reveals repetitive data and movies showing the details of gap closure. As all HoloMonitor applications the wound healing assay is cell-friendly and based on label-free live-cell imaging.

  • Time-lapse images and videos illustrating how cells migrate into the gap
  • Automatic and kinetic calculation of gap closure over time
  • Cell front velocity

To get single cell data and movement patterns, cells of interest can be tracked using cell tracking. Detailed, non-biased data on migration into the wound area is then easily achieved. When preferred, further analyses can be undertaken for details on cell morphology changes, cell proliferation and cell division.

Video showing gap closure over time as the cells fill the gap. Untreated cells are used and the gap is created using the ibidi® Culture-Insert 2 Well.

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HoloMonitor Label-free Wound Healing Assay? 

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