One key benefit of the HoloMonitor technology is that individual cells can be identified and monitored over time.
Unlabeled cells can be studied in the cell incubator, as long as needed to get relevant motility and migration data. This enables both kinetic cell motility studies providing cell population data, as described below, and tracking of single cells, using the Track Cells module.
Explore how cell motility changes
The HoloMonitor® Motility Assay is designed to explore the average motility of cells in a population and is ideal for studies investigating how various treatments, drug candidates, or environments, influence cell motility over time. The cell motility can be long term analyzed using normal cell growth media and environment, with no labeling, staining or phototoxicity.
Captures and automatically presents speed and distance
The HoloMonitor App Suite software automatically analyzes and presents motility data in terms of speed kinetics, i.e. average cell speed over time, and accumulated mean distance, at each time point. Note that the result presentation is immediate and progresses as the experiment proceeds.
The software automatically presents motility data for the cell populations. If preferred all data can easily be exported to Excel for further analysis.
Key References Kinetic Cell Motility
Moving into a New Dimension: Tracking Migrating Cells with Digital Holographic Cytometry in 3DCytometry Part A (2018)Read moreCommentary article discussing the fundamental role of cell movement studies in cancer research. Advantages of the HoloMonitor Track Cells and Wound Healing modules over the transwell migration and invasion assays are highlighted, and includes the possibility to use the cells in experiments for other purposes after completing the imaging. In addition to single cell tracking, the HoloMonitor technology also benefits from the fact that morphology analysis can be performed of each cell. The author concludes that this indeed opens up for almost unlimited possibilities to perform cell morphology analysis using this methodology, since each image is very rich in cellular information.
Evaluation of Holographic Imaging Cytometer HoloMonitor M4 Motility ApplicationsCytometry Part A (2018)Read more
The HoloMonitor software modules for cell tracking and wound healing analysis were evaluated and compared to the more conventional methods transwell migration and transwell invasion. Both HoloMonitor modules were found to be well-correlated with established standards, yielded reproducible results, and at the same time offered distinct advantages. The wound healing assay was the most tractable and automated method with good reproducibility, while the cell tracking module enabled identification of hypermobile subpopulations.
TRPM2 Modulates Neutrophil Attraction to Murine Tumor Cells by Regulating CXCL2 ExpressionCancer Immunology, Immunotherapy (2018)Read more
The tumor microenvironment in vivo is not limited to the tumor cells themselves but also includes many kinds of immune cells. In this study the researchers explored cytotoxic effects of neutrophils in a co-culture with murine tumor cells. To exert their cell killing effect the neutrophils must reach the target cells, i.e. tumor cells. Using the HoloMonitor cell motility and migration assays the scientists could explore how the ability of the neutrophils to approach the cancer cells was affected during various conditions. Their findings are also supported and visualized in an associated time-lapse movie.