Kinetic Motility
One key benefit of the HoloMonitor technology is that individual cells can be identified and monitored over time.
Unlabeled cells can be studied in the cell incubator, as long as needed to get relevant motility and migration data. This enables both kinetic cell motility studies providing cell population data, as described below, and tracking of single cells, using the Track Cells module.
Explore how cell motility changes
The HoloMonitor® Motility Assay is designed to explore the average motility of cells in a population and is ideal for studies investigating how various treatments, drug candidates, or environments, influence cell motility over time. The cell motility can be long term analyzed using normal cell growth media and environment, with no labeling, staining or phototoxicity.
Captures and automatically presents speed and distance
The HoloMonitor App Suite software automatically analyzes and presents motility data in terms of speed kinetics, i.e. average cell speed over time, and accumulated mean distance, at each time point. Note that the result presentation is immediate and progresses as the experiment proceeds.
The software automatically presents motility data for the cell populations. If preferred all data can easily be exported to Excel for further analysis.
Assessment of kinetic motility of a cell population
HoloMonitor® Kinetic Motility Assay:
- Is designed to explore the motility of a cell population over time.
- Is ideal for detection of differences in cell motility causes by the investigated drug or environmental condition vs controls.
- Automatically calculates and presents 1. kinetic motility data in terms of mean speed of all cells in the population, and 2. accumulated mean distance at each time point.
After imaging the undestroyed cells, or the supernatant, can be used for additional analysis using other methods.
Experiments set up using App Suite software can easily be re-analyzed to generate results for another assay type. For example, for a motility experiment, proliferation results can be achieved from the same experimental data.
Motility and migration studies of single cells, or subpopulations of cells, can be performed using the Track Cells module.
- PHI HoloMonitor M4 with App Suite software
- Culture vessels and PHI vessel holder for selected vessel
- PHI HoloLid for selected vessel