Our [single-cell tracking] data clearly show that there are subpopulations of rapidly dividing cells, hiding in population-based data such as the population doubling time. Thus, by only drawing conclusions based on population data, important biological processes on individual cell levels may be neglected.
HoloMonitor M4 was used to study and classify melanocyte cell morphology differences during various cell cycle stages.
HoloMonitor M4 was used to study cell motility, migration and proliferation when cultured on various nanostructured surfaces.
HoloMonitor M4 was used to study cell migration changes in wound healing experiments in after silencing NEXN gene in human coronary artery smooth muscle cells.
HoloMonitor M4 was used to study cell motility and migration after exposure to III-V semiconductor nanowires.
In this thesis, cell division and cell movement of six different cell lines have been studied using digital holographic microscopy. A new way of analysing individual cells by using digital holographic microscopy alone, or in combination with fluorescence microscopy is presented.
HoloMonitor M4 was used to study murine Mesenchymal Stem Cell motility, migration, and speed, while studying gap closure in a scratch assay.
The HoloMonitor M4 was used to monitor living cells (human embryonic kidney cells and preosteoblasts) on nanostructured coatings in a novel cuvette configuration.