Malmö University

Peer Reviewed Articles and Book Chapters

• Non-invasive, Label-free Cell Counting and Quantitative Analysis of Adherent Cells Using Digital Holography

  Anna Mölder et al

  Journal of Microscopy (2008)

  Using a unique non-invasive label-free cell counting method, results comparable to conventional cell counting using a haemocytometer were produced. The major advantage using HoloMonitor™ M2 is the opportunity to easily access information about cell number, size, optical thickness and confluence in an automatic, non-invasive manner.

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• Digital Holographic Microscopy — Innovative and Non-destructive Analysis of Living Cells

  Z. El-Schich, A. Mölder, M. Sebesta, L. Gisselsson, K. Alm, A. Gjörloff Wingren

  Microscopy: Science, Technology, Applications and Education (2010)
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• Digital Holography and Cell Studies

  K. Alm, H. Cirenajwis, L. Gisselsson, A. Gjörloff Wingren, B. Janicke, A. Mölder, S. Oredsson, J. Persson

  Holography-Research and technologies (2011)
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• Cells and Holograms — Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells

  K. Alm, Z. El-Schich, M. Falck Miniotis, A. Gjörloff Wingren, B. Janicke and S. Oredsson

  Holography — Basic Principles and Contemporary Applications  (2013)
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• Digital Holographic Microscopy for Non-invasive Monitoring of Cell Cycle Arrest in L929 Cells

  Maria Falck Miniotis, Anthonny Mukwaya and Anette Gjörloff Wingren

  PLOS ONE (2014)

  We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.

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• Interfacing Antibody-based Microarrays and Digital Holography Enables Label-free Detection for Loss of Cell Volume

  Zahra El-Schich, Emmy Nilsson, Anna S Gerdtsson, Christer Wingren and Anette Gjörloff Wingren

  Future science oa (2015)

  The article provides proof of concept for using holographic microscopy combined with antibody-based microarray technology for detecting morphological changes in captured cells.

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• Supervised Classification of Etoposide-treated in Vitro Adherent Cells Based on Noninvasive Imaging Morphology

  Anna Leida Mölder, Johan Persson, Zahra El-Schich, Silvester Czanner, Anette Gjörloff-Wingren

  Journal of Medical Imaging (2017)

  The morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we report a best case sensitivity of 88% and specificity of 94% for classification of cells as treated/untreated.

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• Holography: the Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

  Zahra El-Schich, Sofia Kamlund, Birgit Janicke, Kersti Alm and Anette Gjörloff Wingren

  Holographic Materials and Optical Systems (2017)

  Open access book chapter discussing the potential of holographic microscopy in clinical applications

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• Quantitative Phase-contrast Imaging – a Potential Tool for Future Cancer Diagnostics

  Anette Gjörloff-Wingren

  Cytometry Part A (2017)

  Prof. Gjörloff-Wingren has used holographic microscopy in cancer related research for over a decade. Gjörloff-Wingren discuss the emerging field of quantitative phase imaging and the novel label-free capabilities that promise to improve and succeed invasive laborious photochemical procedures

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• Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells – Focus on Digital Holographic Microscopy

  Z. El-Schich, A Leida Mölder and A Gjörloff Wingren

  Appl Sci (2018)

  Review providing a current insight into QPI (quantitative phase imaging) applied to cancer research. The authors conclude that QPI is an ideal method for studying live cell dynamics by providing data from noninvasive monitoring over arbitrary time scales. The effect of drugs on migration, proliferation, and apoptosis of cancer cells are emerging fields suitable for QPI analysis. The authors also point out that need for QPI applications in clinical cancer diagnostics and treatments is emerging.

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• Moving into a New Dimension: Tracking Migrating Cells with Digital Holographic Cytometry in 3D

  A. Görloff Wingren

  Cytometry Part A (2018)
  Commentary article discussing the fundamental role of cell movement studies in cancer research. Advantages of the HoloMonitor Track Cells and Wound Healing modules over the transwell migration and invasion assays are highlighted, and includes the possibility to use the cells in experiments for other purposes after completing the imaging. In addition to single cell tracking, the HoloMonitor technology also benefits from the fact that morphology analysis can be performed of each cell. The author concludes that this indeed opens up for almost unlimited possibilities to perform cell morphology analysis using this methodology, since each image is very rich in cellular information.

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