HoloMonitor M4: A live cell imaging system for cell incubators or hypoxia chambers

HoloMonitor® Live Cell Imaging System

Plain HoloMonitor logo in PHI blue

The HoloMonitor® live cell imaging system is a complete label-free, single-cell level imaging solution for your standard CO₂ incubator. It comprises the compact HoloMonitor cell culture microscope along with the tailor-made and powerful App Suite software. Built for automatic cell imaging and analysis, designed by, and for, cell biologists.

Read more about HoloMonitor in its role as…

The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

A Cell Culture Microscope

The main purpose of HoloMonitor is to monitor, record and analyze your cell cultures in the least invasive way possible.

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The HoloMonitor microscope photographed from the side, placed inside a standard incubator.

An Incubator Microscope

The compact HoloMonitor microscope is built to work inside your standard incubator. Thus, it ensures stable and physiologically relevant conditions.

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Zoomed view of yellow L929 cells on a bright blue background

A Live Cell Imaging System

HoloMonitor does not need any labels or stains to visualize your cells. Hence, it has minimal impact on your cells, allowing long-term live cell monitoring.

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Why HoloMonitor?


  • Avoid known interference of cellular markers for biologically relevant results.


  • Small footprint to bring imaging inside your incubator.


  • Not all live cell imaging microscopes have to be expensive.


  • Get immediate real-time data and image sequences.


  • Intuitive software to guide you from imaging to analysis.


  • Controlled conditions matter for your cell culture.
The HoloMonitor live cell imager viewed from the front

HoloMonitor system starting price €26,945

The HoloMonitor Hardware

Base unit of the HoloMonitor cell culture microscope

Imaging Unit

The base unit is the actual microscope, placed in your incubator and responsible for continuously imaging your live cell cultures.

HoloMonitor base unit with the motorized stage quantitative cell imaging

Motorized Stage

The motorized stage supports a variety of culture vessels. Further on, it enables automated and continuous imaging of multiple positions in your sample.

The laser unit of the HoloMonitor live cell imaging system

Laser Unit

The laser unit is placed outside your incubator and provides a low-energy laser to enable holographic imaging without affecting your cells.

Technical Specifications

Technical Information

Sample stageFixed
Light sourceExternal laser unit, 635 nm
Sample illumination635 nm, 0.2 mW/cm²
Lateral resolution1 µm
Field of view0.25 mm²
Working distance0.5–2.0 mm
Autofocusing range1.5 mm
Maximum image rate1 image/s
Image size1024 × 1024 pixels
Dimensions290 × 170 × 185 mm (W × D × H)
Weight3.75 kg
Cell culture vessels6-well, 24-well, 96-well, Petri, IBIDI
Power supplyInternational 110–240 V
Interface cableUSB
Warranty1 year

Sarstedt 35 mm Petri dishes
Sarstedt 6-well plates
Sarstedt Lumox 24-well plates
Sarstedt Lumox 96-well plates
IBIDI plastic ware (channel slides, chemotaxis slides, wound healing inserts)

We always recommend using our HoloLids™ for optimal optical performance.

Regulatory Compliance

Electromagnetic compatibility2014/30/EU and 2014/35/EU (low voltage directive)
Safety requirementsIEC 61010-1:2010 (electrical equipment for measurement, control and laboratory use)
Safety of laser productsIEC 60825-1:2014

CE marking is subject to the general principles set out in Article 30 of Regulation (EC) No 765/2008.


Sample and environment

CellsMonolayer of adherent cells
IncubatorAccess port for cabling
Operating temperature10–40°C
Operating humidityMax 95%


Operating system
Operating systemWindows 10, 64-bit
ProcessorIntel Core i7
Memory16 GB RAM (8 GB minimum)
Hard drive512 GB SSD (256 GB minimum) and external USB3 hard drive for backup and transfer
DisplayFull HD (1920 × 1080) or higher

For service purposes, it is convenient if the computer has internet access. However, we recommend disabling the internet connection during experiments, as automatic Windows updates may disrupt them.

Technical Information

Repeatability5 µm
Travel range100 × 70 × 10 mm (X × Y × Z)
Dimensions*290 × 200 × 185 mm (W × D × H)
Operational dimensions*375 × 260 × 185 (W × D × H)
Minimum space inside the incubator400 × 270 × 185 (W × D × H)
Weight*5.5 kg
Operating temperature10–40°C
Operating humidityMax 95%
Cell culture vessel support6-well, 24-well, 96-well, Petri, IBIDI
Warranty1 year

*Including the base unit

Technical Information

Exposure time5 ms, non-scanning
Light sourceExternal laser unit, 635 nm
Sample illumination0.2 mW/cm²
Power supplyInternational 110–240 V
Interface cableUSB
Warranty1 year

The HoloMonitor Software

The heart of HoloMonitor is the modular App Suite software, with tailor-made apps for different live cell assays. Each app offers a guided setup of your experiment, automatic image collection and real-time data analysis and presentation.

Screenshot of App Suite showing buttons with assay names on a white background.

Moreover, HoloMonitor App Suite excels when it comes to data reanalysis. Every experiment contains all data necessary to reanalyze it as another type of experiment. For example, a proliferation assay can be reanalyzed as a motility assay at a later point. Hence, no need to spend time, money and cells on a new experiment!

HoloMonitor Live Cell Assays

The Workflow

Designed by cell biologists, the HoloMonitor App Suite software offers a simple and guided user interface. So, with a self-explanatory workflow, it guides you from experiment setup to data presentation in 5 simple steps:

HoloMonitor App Suite - live cell imaging and data analysis software
  1. Assay selection
  2. Basic setup
  3. Image capture setup
  4. Start capturing images
  5. View the results in real-time
Workflow of the HoloMonitor cell imaging software, App Suite

In addition, you can easily create colorful videos and images to present your results. Of course, all data can also be exported to Microsoft Excel for further analysis.

Best microscope I’ve tried so far—worth every penny!

Markus Bosteen
Senior Scientist, Novo Nordisk, Copenhagen

HoloMonitor Accessories

24-well HoloLid and plate mounted on plate holder

Culture Vessel Holders

HoloMonitor’s motorized stage supports a variety of culture vessels, each secured with a vessel holder. This means easy attachment and exact positioning on the microscope.

Hand placing 6-well HoloLid on 6-well microplate


HoloLids are specially designed lids for standard cell culture vessels. They are optically superior to normal vessel lids and ensure the best possible image quality every time.

The HoloDry canister together with the included timestrip


HoloDry is a non-toxic canister that is easily attached to the HoloMonitor microscope. It keeps the optics and interior free of condensation, which ensures optimal performance.

Phase Holographic Imaging PHI AB
Skiffervägen 48
224 78 Lund
+46 46 38 60 80

Phase Holographic Imaging PHI Inc.
265 Franklin Street Suite 1702
Boston, MA 02110
+1 617-963-5150


VAT: SE556542781101

Securities Identification

ISIN: SE0005504636
LEI: 549300JZR79QYESM6296

HoloMonitor® is a registered trademark
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Phase Holographic Imaging PHI AB

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