HoloMonitor ® — An Incubator Microscope

The HoloMonitor incubator microscope facilitates non-invasively cell culture analysis on a single-cell level in real-time. HoloMonitor operates within a CO2 incubator or hypoxia chamber, enabling long-term studies of in vitro cell populations in a stable and physiologically relevant environment.

The HoloMonitor live cell imaging microscope system provides more than 30 cell parameters, reflecting cell health, proliferation, migration, differentiation and cell cycle phase.

HoloMonitor® M4 operates in a cell incubator, hypoxia chamber or on the lab bench.

Single-cell analysis on a population level

From just a single cell culture experiment you can learn how treatments affect the response and behavior of your cell culture samples.

HoloMonitor is an incubator tolerant phase imaging microscope. This allows HoloMonitor to provide label-free cell culture analysis in an optimal environment over extended periods of time. HoloMonitor quantifies more than 30 single-cell parameters on a population level, reflecting:

  • cell morphology,
  • cell proliferation,
  • cell motility and migration,
  • cell invasion,
  • cell differen­ti­a­tion,
  • cell cycle phase and other cellular events.

Cell-friendly long-term imaging & analysis

HoloMonitor deploys quantitative phase contrast microscopy to non-invasively analyze cellular events by long-term digital phase imaging. While recording, the time-lapse image sequences are automatically analyzed to in real-time provide single-cell and population information in the form of graphs and scatterplots.

One sample, multiple results

By reanalyzing a previously recorded image sequence, multiple results can be obtained from a single cell culture sample using the HoloMonitor cell imaging applications — saving time, effort and precious stem and primary cells.

From within the cell incubator, HoloMonitor records multiple time-lapse image sequences of a cell culture sample. By letting several HoloMonitor App Suite applications analyze the stored image sequences, a multitude of results is obtained from a single sample. The data analysis can be done anywhere on a standard laptop, which has access to the HoloMonitor database.

First point of investigation

Contrary to fluorescence microscopy, the cells are non-invasively imaged with a low power laser without labels or stains. This leaves the cells completely unaffected for further downstream studies using complementary instrumentation, for example, fluorescent imaging by high-resolution confocal microscopy.

Workflow of the HoloMonitor live cell imaging system

In this cell culture analysis webinar Kersti Alm, Head of Biology at PHI, demonstrates how to quantify cell movements and relate them to other cellular parameters, such as cell size and shape.

She shares results of the latest publications by scientists using HoloMonitor and highlights the great potential of simultaneously quantifying cell movement and morphology, for both single-cells and cell populations.

An incubator microscope — for and by cell biologists

Designed by cell biologists, the HoloMonitor App Suite cell imaging software offers a simple and guided user interface with self-explanatory workflow, from experiment setup through data analysis. In addition, you can also easily create colorful videos and images. All data can of course be exported to Microsoft Excel for further analysis.

Customers employ the HoloMonitor incubator microscope for cell culture analysis in a variety of fields within cell biology, including cancer research, drug discovery and stem cell research. To date, research results have been published in more than 150 peer-reviewed publications.

The HoloMonitor App Suite live cell imaging and analysis software intuitively guides the user through experiment setup, image acquisition and data analysis.

HoloMonitor App Suite intuitively guides the user through experiment setup, image acquisition and data analysis.

System components

  • Microscope unit

    The HoloMonitor microscope unit can be permanently installed directly in a cell incubator, in a hypoxia chamber or on the lab bench.

  • High precision motorized stage

    HoloMonitor is equipped with a motorized XYZ-stage. With this high precision stage, HoloMonitor analyzes and records time-lapse image sequences at multiple locations in selected wells.

  • Low power illumination

    The illumination source is a low power 635-nm diode laser unit, placed outside the incubator and connected to the microscope unit with fiber optics.

  • Condensation free lids

    The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

Showing the HoloMonitor imaging system with a motorized xyz-stage and microplate holder

Sample holders are available for microplates, slides and 35 mm petri dishes

The low power HoloMonitor laser unit is placed outside the incubator and connected to the HoloMonitor base unit with fiber optics.

The HoloMonitor laser unit

The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

HoloMonitor HoloLid for 6-well microplates

Sample Requirements

  • The HoloMonitor assays neither require fluorescent proteins or labels, nor any specially designed vessels, making protocols straightforward and easy-to-use.
  • A monolayer of adherent eukaryotic primary cells or cell line cells seeded in any of the recommended vessels.
  • Customers have successfully used HoloMonitor with a large number of cell types, see peer-reviewed publication list.

“The HoloMonitor live cell imaging microscope quantifies living cells by measuring how light changes direction when passing through unstained cells. The cells are completely unaffected, as no light energy whatsoever is absorbed or transferred to the cells — no energy exchange, no change”

Prof Stina_Oredsson

HoloMonitor gives a totally new dimension to our work

Prof. Stina Oredsson

Lund University