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HoloMonitor M4 — Live Cell Imaging in Your Incubator

HoloMonitor® is a time-lapse cytometer for label-free in vitro live cell imaging and analysis, directly in your CO2 cell incubator.

HoloMonitor is used by scientists all over the world to study the life cycle and behavior of living — or dying — cells, under physiologically optimal conditions. No stains needed.

The employed technique, holographic microscopy, is cell-friendly, fast and simple to use, offering unique imaging capabilities for live-cell kinetic investigations, at both single-cell and cell-population levels.

The HoloMonitor M4 live cell imaging system is designed to visualize and quantify adherent cells over time. The system software, App Suite, allows researchers to create publication-ready images, videos and diagrams of cellular behavior, using a range of applications  — all label-free.

Apoptotic cell explosion

Visualizes and Quantifies Cellular Events

HoloMonitor is designed to generate both visual elements and quantitative data. Visual elements, such as sequences of images and videos, illustrate cellular events taking place during the experiment, while quantitative data put numbers on what can be seen with the human eye.

From visual information in the holographic images, the data are translated by sophisticated software algorithms into morphological parameters — optical cell volume, thickness, texture and many more — to provide kinetic cell analyses.

Compared to standard methods the HoloMonitor approach saves both time, money and valuable cells for further experiments.

Learn more how HoloMonitor can save time and money — and your cells here.

  • Reliable and Cost-effective Operation

    Since no labels or stains are needed, the assay protocols are straight forward and the experiments are quick and easy to set-up. A new and innovative mechanical design together with an intuitive software interface makes HoloMonitor M4 operation simple and reliable.

  • Ideal for Kinetic Cell Population Studies

    Images can be recorded with high temporal resolution (maximum image rate: 1 image/s), ensuring that important cellular events are not overlooked. This high temporal resolution is one key reason why HoloMonitor technology is optimal for kinetic studies to advance our understanding of processes such as cell growth, movements, division, and death.

  • Enabling Long-term Experiments

    The incubator-compatible design makes the HoloMonitor M4 system especially suited for long-term kinetic cellular analyses. The low-intensity, single-wavelength laser generates no heat reducing the risk of photo-damage to an absolute minimum.

HoloMonitor M4: live cell imaging in cell incubator

Cell Friendly and Incubator-compatible Live Cell Imaging

All HoloMonitor applications are totally label free; no staining or contrast agents are required. Cell cultures can be continuously monitored and analyzed under physiologically optimal conditions, i.e. in the cell incubator or hypoxia chamber, before and after treatment with drug candidates or other chemical compounds, minute by minute and day after day, without the need for any stains or labels. The risk for toxic effects and/or masking of the effect of various conditions and/or target drug is thus avoided.

HoloMonitor M4 adds a new dimension to our portfolio of complex imaging technologies — TIME!

Ed Luther, Supervisor

Core Imaging and Cytometry Facility, Northeastern University in Boston