The HoloMonitor live cell analysis system operates in your CO2 cell incubator environment, hypoxia chamber or directly on the lab bench.
HoloMonitor is used by scientists all over the world to study the life cycle and behavior of living — or dying — cells, under physiologically optimal conditions. No stains needed. The non-invasive core technology, holographic microscopy, is cell-friendly, fast and simple to use, offering unique imaging capabilities for live cell kinetic investigations at single-cell and cell population level.
Developed by and for cell biologists, HoloMonitor is designed to in vitro visualize and quantify adherent cells in real-time in a cell incubator environment. The App Suite imaging and live cell analysis software allows researchers to create publication-ready images, videos and diagrams of cellular drug response and behavior, using a range of applications — all label-free.
An apoptotic explosion imaged by HoloMonitor
HoloMonitor generates both visual elements and quantitative data. Visual elements, such as sequences of images and videos, illustrate cellular events taking place during the experiment, while quantitative data put numbers on what can be seen with the human eye.
From the visual information in the holographic microscope images, the data is translated by sophisticated software algorithms into morphological parameters — optical cell volume, thickness, texture and many more — to provide kinetic live cell analysis in your cell incubator in real-time.
Beatrix Peter et al., Incubator proof miniaturized Holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections, SPIE (2015)
HoloMonitor and incubator-based live cell imaging allow cell cultures to be continuously monitored and analyzed over extended periods time under physiologically optimal conditions — in a standard CO2 incubator or a hypoxia chamber. As all HoloMonitor applications are completely label-free, the risk of toxic effects masking drug-induced effects is reduced to a minimum.
Since no labels or stains are needed, the assay protocols are straight forward and the experiments are quick and easy to set-up. A new and innovative mechanical design together with an intuitive software interface makes HoloMonitor operation simple and reliable.
Images can be recorded with high temporal resolution, ensuring that important cellular events are not overlooked. This high temporal resolution is a key reason why HoloMonitor technology is optimal for kinetic live cell studies to advance the understanding of processes such as cell growth, movements, division and cell death.
The incubator-compatible design makes the HoloMonitor system especially well suited for long-term kinetic cellular analyses. The low-intensity single-wavelength laser generates no heat, reducing the risk of photo-damage to an absolute minimum.
HoloMonitor in a standard CO2 cell incubator
Do you wonder how live cell imaging with HoloMonitor® fits your research needs?
We can image live cells from our lab for you or show the ease of data analysis with our wide application portfolio.
Our product experts and application specialists are happy to answer your questions.
HoloMonitor M4 adds a new dimension to our portfolio of complex imaging technologies — TIME!
Core Imaging and Cytometry Facility, Northeastern University in Boston