Real-time Live Cell Analysis

The HoloMonitor live cell analysis system operates in your CO2 cell incubator environment, hypoxia chamber or directly on the lab bench.

HoloMonitor is used by scientists all over the world to study the life cycle and behavior of living — or dying — cells, under physio­logi­cally optimal conditions. No stains needed. The non-invasive core technology, holographic microscopy, is cell-friendly, fast and simple to use, offering unique imaging capabilities for live cell kinetic investigations at single-cell and cell population level.

Developed by and for cell biologists, HoloMonitor is designed to in vitro visualize and quantify adherent cells in real-time in a cell incubator environment. The App Suite imaging and live cell analysis software allows researchers to create publi­cation-ready images, videos and diagrams of cellular drug response and behavior, using a range of applications  — all label-free.

Apoptotic cell explosion

An apoptotic explosion imaged by HoloMonitor

Visualizes and Quantifies Cellular Events

HoloMonitor generates both visual elements and quantitative data. Visual elements, such as sequences of images and videos, illustrate cellular events taking place during the experiment, while quantitative data put numbers on what can be seen with the human eye.

From the visual information in the holographic microscope images, the data is translated by sophisticated software algorithms into morphological parameters — optical cell volume, thickness, texture and many more — to provide kinetic live cell analysis in your cell incubator in real-time.

Live Cell Analysis for Cell Incubators and Hypoxia Chambers

  • Cell-friendly

    HoloMonitor and incubator-based live cell imaging allow cell cultures to be continuously monitored and analyzed over extended periods time under physiologically optimal conditions — in a standard CO2 incubator or a hypoxia chamber. As all HoloMonitor applications are completely label-free, the risk of toxic effects masking drug-induced effects is reduced to a minimum.

  • Reliable and Cost-effective Operation

    Since no labels or stains are needed, the assay protocols are straight forward and the experiments are quick and easy to set-up. A new and innovative mechanical design together with an intuitive software interface makes HoloMonitor operation simple and reliable.

  • Single-cell Analysis on a Population Level

    Images can be recorded with high temporal resolution, ensuring that important cellular events are not overlooked. This high temporal resolution is a key reason why HoloMonitor technology is optimal for kinetic live cell studies to advance the understanding of processes such as cell growth, movements, division and cell death.

  • Long-term Live Cell Imaging Experiments

    The incubator-compatible design makes the HoloMonitor system especially well suited for long-term kinetic cellular analyses. The low-intensity single-wavelength laser generates no heat, reducing the risk of photo-damage to an absolute minimum.

HoloMonitor M4: A live cell imaging system for cell incubators or hypoxia chambers

HoloMonitor in a standard CO2 cell incubator


HoloMonitor M4 adds a new dimension to our portfolio of complex imaging technologies — TIME!

Ed Luther, Supervisor

Core Imaging and Cytometry Facility, Northeastern University in Boston