HoloMonitor M4 — Live Cell Imaging in Your Incubator
HoloMonitor® is a time-lapse cytometer for label-free in vitro live cell imaging and analysis, directly in your CO2 cell incubator.
HoloMonitor is used by scientists all over the world to study the life cycle and behavior of living — or dying — cells, under physiologically optimal conditions. No stains needed.
The employed technique, holographic microscopy, is cell-friendly, fast and simple to use, offering unique imaging capabilities for live-cell kinetic investigations, at both single-cell and cell-population levels.
The HoloMonitor M4 live cell imaging system is designed to visualize and quantify adherent cells over time. The system software, App Suite, allows researchers to create publication-ready images, videos and diagrams of cellular behavior, using a range of applications — all label-free.
Visualizes and Quantifies Cellular Events
HoloMonitor is designed to generate both visual elements and quantitative data. Visual elements, such as sequences of images and videos, illustrate cellular events taking place during the experiment, while quantitative data put numbers on what can be seen with the human eye.
From visual information in the holographic images, the data are translated by sophisticated software algorithms into morphological parameters — optical cell volume, thickness, texture and many more — to provide kinetic cell analyses.
Compared to standard methods the HoloMonitor approach saves both time, money and valuable cells for further experiments.
Cell Friendly and Incubator-compatible Live Cell Imaging
All HoloMonitor applications are totally label free; no staining or contrast agents are required. Cell cultures can be continuously monitored and analyzed under physiologically optimal conditions, i.e. in the cell incubator or hypoxia chamber, before and after treatment with drug candidates or other chemical compounds, minute by minute and day after day, without the need for any stains or labels. The risk for toxic effects and/or masking of the effect of various conditions and/or target drug is thus avoided.
Ed Luther, Supervisor
Core Imaging and Cytometry Facility, Northeastern University in Boston