Customer Publication
Lactobacilli-host interactions inhibit Staphylococcus aureus and Escherichia coli-induced cell death and invasion in a cellular model of infection
Journal: Frontiers in Microbiology (2024)
Institution: Department of Molecular Biology and Genetics, Democritus University of Thrace, Greece
Research Areas: Infection Response
Cell Lines: HT-29 (human colorectal adenocarcinoma)
Summary: Probiotic lactobacilli exert antimicrobial and antibiofilm activity through a plethora of mechanisms, including the production of inhibitory compounds and competition for nutrients and adhesion sites on the host mucosa. Their ability to interfere with pathogen-host interactions may mitigate the harmful effects of infection. The present study aims to investigate their ability to limit cell death induced by Staphylococcus aureus (SA) and Escherichia coli (EC) in the human colon adenocarcinoma cell line HT-29. Lactobacilli treatment down-regulated pathways involved in pathogen adhesion and internalization, endocytosis, cell–cell and cell-extracellular matrix (ECM) adhesion in HT-29 cells, resulting in pathogen protection. Our findings highlight the strain-specific protective effects of LAB against pathogen-induced cell death achieved through competitive exclusion and priming of host cell responses. Using the kinetic proliferation assay, HoloMonitor-generated data showed the decrease in cell proliferation caused by treatment of HT-29 cells with SA or EC.