Authors: Taehoon Oh et al.

Journal: Nutrients (2025)

Institution: Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju 28116, Republic of Korea; dhxogns10@kribb.re.kr

Research Areas: Cell Research

Cell Lines: MEF (Mouse fibroblast cell)

Summary: the authors investigated a traditional vinegar-soaked egg extract (vinegar-egg) to identify phenolic compounds that may enhance wound healing. They isolated four compounds (4-hydroxybenzoic acid, vanillic acid, methyl syringate, leptosperin) and found that the first two significantly enhanced cell motility and invasion in fibroblast models, correlating with increased phosphorylation of focal adhesion kinase (FAK), MMP-2 and p38 MAPK signalling. To monitor dynamic cell behaviour, they employed the HoloMonitor M4 to track individual adherent cells in real time (label-free) and thereby quantify motility/migration changes under treatment. The goal was to link molecular signalling events to functional cell behaviour, the results show that vinegar-egg derived phenolics can enhance wound-healing-relevant cellular behaviour.

Keywords: HoloMonitor M4, single cell tracking, vinegar-egg, 4-hydroxybenzoic acid, vanillic acid, wound healing, cell motility, migration, cell invasion, focal adhesion kinase (FAK), MAPK, p38