Customer Publication
In situ monitoring of cell adhesion kinetics using label-free optical biosensors: Evaluating anticancer potential and selectivity of natural compounds
Journal: View (2025)
Research Areas: Drug Discovery
Cell Lines: Hela, MC3T3-E1 (Cervical cancer cells, preosterblast cells)
Summary: This study presents a novel label-free approach for identifying natural compounds with anticancer potential by continuously monitoring their effects on cell adhesion and morphology. Using a resonant waveguide grating biosensor and HoloMonitor, the researchers analyzed 11 pure natural compounds and four crude extracts on cancerous (HeLa) and healthy (preosteoblast) cells in real time. The combined methods allowed simultaneous assessment of adhesion kinetics and cell morphology, revealing both the potency and mechanisms of the compounds. They found that aryltetralin lignans (deoxypodophyllotoxin and angeloyl podophyllotoxin) exerted non-selective anti-adhesive effects through direct interaction with fibronectin, while other compounds like balanophonin and vermelhotin selectively inhibited cancer cell adhesion through fibronectin-independent mechanisms.HoloMonitor M4 was used to study the morphology change in response to adhesion-inhibition treatment.