Cell Death Kinetics
The Process of Cell Death
Cell toxicity, or cytotoxicity, is defined as the ability of certain substances or mediator cells to destroy living cells. Exposure of cells to a cytotoxic compound is ultimately lethal and induces either necrosis, during which the cells lose membrane integrity and die rapidly, or apoptosis (programmed cell death). Cell death, in terms of cell viability, is thus a common end point in toxicological studies. However, cells usually undergo morphological changes before the actual cell death occurs. Healthy cells are often irregular and rather thin, dying cells are small, less irregular and thick, while dead cells are round and thin. Analyzing these features thus enables detailed understanding of the cell death process, both for individual cells and cell populations.
DU-145 prostate cancer cell undergoing apoptosis. The graph illustrates how morphological changes, in this case in cell volume and thickness, precede the actual cell death.
The graph is an example showing how morphological parameters can differentiate between untreated cells and cells treated with an cytotoxic substance. In this case cell thickness is plotted vs irregularity. Green dots represent untreated, and blue and red represent etoposide-treated L929 cells.