The HoloMonitor Live Cell Imaging System

The HoloMonitor® live cell imaging system non-invasively monitors the response and behavior of cell culture populations in real-time on a single-cell level. The label-free imager operates within a CO2 incubator or hypoxia chamber, enabling long-term analysis of living cell populations in a stable and physiologically relevant environment.

The HoloMonitor live cell imaging microscope system provides more than 30 cell parameters, reflecting cell health, proliferation, migration, differentiation and cell cycle phase.

HoloMonitor® M4 operates in a cell incubator, hypoxia chamber or on the lab bench.

Single-cell imaging on a population level

From just a single live cell imaging experiment you can learn how treatments affect the response and behavior of your cell culture samples.

HoloMonitor is a quantitative phase contrast microscope. Without requiring invasive labels or stains, this allows HoloMonitor to provide more than 30 cellular parameters on a population or single-cell level, reflecting:

  • cell morphology,
  • cell proliferation,
  • cell motility and migration,
  • cell invasion,
  • cell differen­ti­a­tion,
  • cell cycle phase and other cellular events.

Cell-friendly long-term imaging & analysis

HoloMonitor deploys quantitative phase contrast microscopy to non-invasively analyze cellular events by long-term digital imaging. While recording, the time-lapse image sequences are automatically analyzed to in real-time provide single-cell and population information in the form of graphs and scatterplots.

By reanalyzing a previously recorded image sequence using the HoloMonitor imaging applications, multiple results can be obtained from a single sample — saving time, effort and precious stem and primary cells.

Contrary to fluorescence microscopy, the cells are non-invasively imaged with a low power laser without labels or stains. This leaves the cells completely unaffected for further downstream studies using complementary instrumentation, for example, fluorescent imaging by high-resolution confocal microscopy.

In this live cell imaging webinar Kersti Alm, Head of Biology at PHI, showcases you how, by using label-free live cell imaging, you can quantify cell movements and relate them to other cellular parameters such as cell size and shape.

She shares results of latest publications from researchers worldwide using HoloMonitor time-lapse cytometer and highlight the great potential of quantifying cell movement and morphology, for both single-cell and cell populations.

Live cell imaging for and by cell biologists

Designed by cell biologists, the HoloMonitor App Suite software offers a simple and guided user interface with self-explanatory workflow, from experiment setup through data analysis. In addition, you can also easily create colorful videos and images. All data can of course be exported to Microsoft Excel for further analysis.

Customers employ the HoloMonitor microscopy system in a variety of fields within cell biology, including cancer research, drug discovery and stem cell research. To date, research results have been published in more than 150 peer-reviewed publications.

The HoloMonitor App Suite live cell imaging and analysis software intuitively guides the user through experiment setup, image acquisition and data analysis.

HoloMonitor App Suite intuitively guides the user through experiment setup, image acquisition and data analysis.

System components

  • Cell imager

    The HoloMonitor cell imager can be permanently installed directly in a cell incubator, in a hypoxia chamber or on the lab bench.

  • High precision motorized stage

    The cell imager is equipped with a motorized XYZ-stage. With this high precision stage, HoloMonitor analyzes and records time-lapse image sequences at multiple locations in selected wells.

  • Low power illumination

    The illumination source is a low power 635-nm diode laser unit, placed outside the incubator and connected to the cell imager with fiber optics.

  • Condensation free lids

    The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

Showing the HoloMonitor imaging system with a motorized xyz-stage and microplate holder

Sample holders are available for microplates, slides and 35 mm petri dishes

The low power HoloMonitor laser unit is placed outside the incubator and connected to the HoloMonitor base unit with fiber optics.

The HoloMonitor laser unit

The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

HoloMonitor HoloLid for 6-well microplates

Sample Requirements

  • The HoloMonitor assays neither require fluorescent proteins or labels, nor any specially designed vessels, making protocols straightforward and easy-to-use.
  • A monolayer of adherent eukaryotic primary cells or cell line cells seeded in any of the recommended vessels.
  • Customers have successfully used HoloMonitor with a large number of cell types, see peer-reviewed publication list.

“The HoloMonitor live cell imaging microscope quantifies living cells by measuring how light changes direction when passing through unstained cells. The cells are completely unaffected, as no light energy whatsoever is absorbed or transferred to the cells — no energy exchange, no change”

Prof Stina_Oredsson

HoloMonitor gives a totally new dimension to our work

Prof. Stina Oredsson

Lund University