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An All in One Live Cell Imaging System

The HoloMonitor® live cell imaging microscope system provides more than 30 cell parameters, reflecting cell health, proliferation, migration, differen­ti­a­tion and cell cycle phase, on a population or single-cell level. From just a single live cell experiment you can learn how treatments affect the response and behavior of your cells.

The HoloMonitor live cell imaging microscope system provides more than 30 cell parameters, reflecting cell health, proliferation, migration, differentiation and cell cycle phase.

Live Cell Imaging in Your Incubator

Directly in the cell incubator, the HoloMonitor microscope system analyzes cell response in real-time by recording time-lapse images. While recording, the live cell image sequences are auto­matically analyzed to immediately provide single-cell and cell population information in the form of graphs, scatterplots and cell images.

By reanalyzing a previously recorded image sequence using additional HoloMonitor applications, multiple results can be obtained from a single cell culture sample — saving time, effort and precious stem and primary cells. As the cells are non-invasively imaged with a low power laser without labels or stains, the cells are completely unaffected for further downstream studies using complementary instrumentation.

Customers employ HoloMonitor for live cell imaging in a variety of fields, including cancer research, drug discovery and stem cell research. To date, research results have been published in more than 150 peer-reviewed publications.

A Live Cell Imaging System — for and by Cell Biologists

HoloMonitor App Suite

Designed by cell biologists, the HoloMonitor App Suite software offers a simple and guided user interface with self-explanatory workflow, from experiment setup through data analysis. In addition, you can also easily create colorful videos and images of your cells. All data can of course be exported to Microsoft Excel for further analysis.

The HoloMonitor App Suite live cell imaging and analysis software intuitively guides the user through experiment setup, image acquisition and data analysis.

HoloMonitor App Suite intuitively guides the user through experiment setup, image acquisition and data analysis.

Explore all the HoloMonitor App Suite applications

Here

HoloMonitor M4 base unit

The base unit of the microscope system can be permanently installed in a cell incubator, hypoxia chamber or on the lab bench.

High precision motorized stage

The base unit is equipped with a motorized XYZ-stage. With this high precision stage, HoloMonitor records time-lapse image sequences at multiple locations in selected cell culture wells.

Low power illumination

The illumination source is a low power 635-nm diode laser unit, placed outside the incubator and connected to the base unit with fiber optics.

Condensation free lids

The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

Showing the HoloMonitor imaging system with a motorized xyz-stage and microplate holder

Sample holders are available for microplates, slides and 35 mm petri dishes

The low power HoloMonitor laser unit is placed outside the incubator and connected to the HoloMonitor base unit with fiber optics.

The HoloMonitor laser unit

The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.

HoloMonitor HoloLid for 6-well microplates

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“The HoloMonitor live cell imaging microscope quantifies living cells by measuring how light changes direction when passing through unstained cells. The cells are completely unaffected, as no light energy whatsoever is absorbed or transferred to the cells — no energy exchange, no change”

HoloMonitor gives a totally new dimension to our work

Prof. Stina Oredsson

Lund University

Cell Sample Requirements

  • No fluorescent labels, stains or specially designed vessels are needed to run HoloMonitor assays, making protocols straight-forward and easy-to-use.
  • A monolayer of adherent eukaryotic primary cells or cell line cells seeded in any of the recommended cell culture vessels.
  • Customers have successfully used HoloMonitor with a large number of cell types, see peer-reviewed publication list here.
Digitally colored L929 cells imaged by HoloMonitor

Digitally colored L929 cells imaged by HoloMonitor