The HoloMonitor® live cell imaging system non-invasively monitors the response and behavior of cell culture populations in real-time on a single-cell level. The label-free imager operates within a CO2 incubator or hypoxia chamber, enabling long-term analysis of living cell populations in a stable and physiologically relevant environment.
HoloMonitor® M4 operates in a cell incubator, hypoxia chamber or on the lab bench.
From just a single live cell imaging experiment you can learn how treatments affect the response and behavior of your cell culture samples.
HoloMonitor is a quantitative phase contrast microscope. Without requiring invasive labels or stains, this allows HoloMonitor to provide more than 30 cellular parameters on a population or single-cell level, reflecting:
HoloMonitor deploys quantitative phase contrast microscopy to non-invasively analyze cellular events by long-term digital imaging. While recording, the time-lapse image sequences are automatically analyzed to in real-time provide single-cell and population information in the form of graphs and scatterplots.
By reanalyzing a previously recorded image sequence using the HoloMonitor imaging applications, multiple results can be obtained from a single sample — saving time, effort and precious stem and primary cells.
Contrary to fluorescence microscopy, the cells are non-invasively imaged with a low power laser without labels or stains. This leaves the cells completely unaffected for further downstream studies using complementary instrumentation, for example, fluorescent imaging by high-resolution confocal microscopy.
Croft et al., Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies, Theranostics (2019)
In this live cell imaging webinar Kersti Alm, Head of Biology at PHI, showcases you how, by using label-free live cell imaging, you can quantify cell movements and relate them to other cellular parameters such as cell size and shape.
She shares results of latest publications from researchers worldwide using HoloMonitor time-lapse cytometer and highlight the great potential of quantifying cell movement and morphology, for both single-cell and cell populations.
Designed by cell biologists, the HoloMonitor App Suite software offers a simple and guided user interface with self-explanatory workflow, from experiment setup through data analysis. In addition, you can also easily create colorful videos and images. All data can of course be exported to Microsoft Excel for further analysis.
Customers employ the HoloMonitor microscopy system in a variety of fields within cell biology, including cancer research, drug discovery and stem cell research. To date, research results have been published in more than 150 peer-reviewed publications.
HoloMonitor App Suite intuitively guides the user through experiment setup, image acquisition and data analysis.
The HoloMonitor cell imager can be permanently installed directly in a cell incubator, in a hypoxia chamber or on the lab bench.
The cell imager is equipped with a motorized XYZ-stage. With this high precision stage, HoloMonitor analyzes and records time-lapse image sequences at multiple locations in selected wells.
The illumination source is a low power 635-nm diode laser unit, placed outside the incubator and connected to the cell imager with fiber optics.
The HoloMonitor HoloLids are specially designed to eliminate image disturbances, caused by surface vibrations and condensation inside the cell culture vessel.
Sample holders are available for microplates, slides and 35 mm petri dishes
The HoloMonitor laser unit
HoloMonitor HoloLid for 6-well microplates
“The HoloMonitor live cell imaging microscope quantifies living cells by measuring how light changes direction when passing through unstained cells. The cells are completely unaffected, as no light energy whatsoever is absorbed or transferred to the cells — no energy exchange, no change”
HoloMonitor gives a totally new dimension to our work
Do you wonder how live cell imaging with HoloMonitor® fits your research needs?
We can image live cells from our lab for you or show the ease of data analysis with our wide application portfolio.
Our product experts and application specialists are happy to answer your questions.