With the HoloMonitor system, cellular behavior, drug responses, and cell events can easily and continuously be visualized and quantified over time. Microscopy images are recorded inside the incubator directly in the cell culture vessel at regular time intervals, to not overlook any significant events.
The HoloMonitor system comprises:
- An incubator-compatible base unit with motorized stage (optional)
- A laser unit
- A proprietary software
The laser and base units are used to capture time-lapse holographic phase images of cells. The system is controlled by two proprietary software programs, Hstudio, and HoloMonitor App Suite.
Prof. Stina Oredsson
M4 base unit
The HoloMonitor M4 base unit can be permanently installed directly in a cell incubator, in a hypoxia chamber, or on the lab bench.
The HoloMonitor M4 base unit can optionally be equipped with a motorized xyz-stage. With this high precision stage, the HoloMonitor M4 can record time-lapse videos at multiple locations in parallel. Sample locations can be within the same culture or in different cultures.
M4 laser unit
The illumination source is a 635-nm diode laser placed outside the incubator and connected with fiber optics
HoloMonitor M4 unit with motorized stage, inside a cell incubator.
The HoloMonitor® App Suite software offers a simple and guided user interface, with self-explanatory work flow, but still allows the user to set precise parameters for each time-lapse experiment.
The Hstudio software allows for flexible set-up of experiments and cytometric analysis of the huge volume of data collected during imaging.
No labels, stains or specially designed vessels are needed to run HoloMonitor assays making protocols straight-forward and easy-to-use.
Mono-layer of adherent eukaryotic cells seeded in any of the recommended cell culture vessels. Download a list of cell lines successfully used: Cell Lines used with HoloMonitor.
- Glass-bottom Petri dishes
- 35 mm Sarstedt Petri dishes with HoloLid
- IBIDI plastic ware (channel slides, chemotaxis slides, wound healing inserts)
- Sarstedt 6-well plates with HoloLid
- Sarstedts Lumox 24 or 96 well plates with HoloLid
Operating system: Windows 10, 64-bit
Processor: Intel Core i7
Memory: 16 GB RAM (8 GB minimum)
Hard drive: 512 GB SSD (256 GB minimum) and external USB3 hard drive for backup and transfer
Display: Full HD (1920x1080) or higher