Non-invasive live cell imaging in your standard incubator

What is live cell imaging?

Live cell imaging is the study of living cells using microscopy. The most common way to study living cells is by using time-lapse imaging, where several subsequent images are collected over time. This allows you to follow your cells and study their behavior over time.

HoloMonitor M4 live cell imaging & analysis system
The HoloMonitor M4 live cell imaging microscope.
HoloMonitor logo without tagline

Live cell imaging with HoloMonitor®

Live cell imaging is where the HoloMonitor microscope excels. Using digital holography, it collects 3D images of your cell cultures completely non-invasively. It requires no labels or stains, and the low-energy laser generates negligible phototoxicity. Placed in your standardincubator, this allows you to collect real-time data on your cell’s unaffected behavior, for as often and as long as you want.

This time-lapse video was recorded with HoloMonitor over 24 hours. It shows MDA-MB-231 breast cancer cells in vitro. The images are captured completely label-free and the colors are added afterward in the computer.

Quantifying over time is crucial for a full under­standing of cell systems. I am convinced that time-lapse cytometry will enable the next level of insight.


Always measures over 30 cellular features

Every image HoloMonitor takes contains position and size information about every cell, in three dimensions. From this, you can extract over 30 different cellular features, such as cell area, thickness, volume and migration speed. Many of the features are unique for digital holography microscopy. If you do a time-lapse, you can study all of these parameters simultaneously over time, at population level and all the way down to single-cell level.

HoloMonitor M4: A live cell imaging system for cell incubators or hypoxia chambers

Non-invasive imaging inside your incubator

The HoloMonitor microscope is designed to be placed inside your standard incubator, lab bench, or hypoxia chamber 24/7, allowing you to image your cells in their natural environment. Furthermore, as it uses digital holography to image your cells, it does not use any kind of labels or stains to visualize them, reducing the imaging influence on cell behavior to a minimum. Taken together, this makes HoloMonitor the perfect, non-invasive live cell imaging tool, unrivaled by the competition.

Result screen of the HoloMonitor App Suite dose response live cell imaging application

Clear but comprehensive data presentation

The HoloMonitor App Suite cell imaging software collects all data on your cells and presents it the way you want. For example, you can get graphs showing cellular changes over time, dot plots to find subpopulations within your cell cultures or just plain numbers if you are just interested in the cell count. Of course, all data can be exported to Microsoft Excel as well, allowing you to do your own calculations and data presentation.

Screenshot showing an optical thickness plot in HoloMonitor App Suite

Strong software with walk-through assays

The HoloMonitor App Suite cell imaging software offers several predefined live cell assays to study, for example, proliferation and morphology. The software guides you through an easy setup, runs the experiment for you, and compiles the results in real time. It even lets you reanalyse your experiments, so if you did a dose-response assay but need motility data, there is no need setup a new assay. Just reanalyze what you already have, saving both time, money and cells.

Live cell imaging with HoloMonitor

Wound healing (scratch assay) with HoloMonitor. The software automatically gives you relevant data such as cell front velocity and gap closure over time.

Single cell tracking with HoloMonitor. Every cell is automatically tracked and the software generates cell trees and motility data on the fly.