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Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy

Cancer cells differ from other cells when it comes to cell morphology, cell proliferation, cell division, cell migration and cell motility. To investigate cell division, the researchers behind this article, used HoloMonitor to track cell movements. Holographic time-lapse movies were used to extract information on cell division of individual cells, and cell family trees were constructed where the cells were categorized. Learn more and read the article here.

Publications

Below you will find summaries and links to peer reviewed articles and book chapters discussing HoloMonitor applications and PHI's core technology – holographic and quantitative phase imaging (QPI) microscopy.

Peer Reviewed Articles and Book Chapters

Highlighted Articles

  • Evaluation of Holographic Imaging Cytometer HoloMonitor M4 Motility Applications
    Y. Zhang and R. L. Judson
    Cytometry Part A (2018)

    The HoloMonitor software modules for cell tracking and wound healing analysis were evaluated and compared to the more conventional methods transwell migration and transwell invasion. Both HoloMonitor modules were found to be well-correlated with established standards, yielded reproducible results, and at the same time offered distinct advantages. The wound healing assay was the most tractable and automated method with good reproducibility, while the cell tracking module enabled identification of hypermobile subpopulations.

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  • Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation
    H. Zeng et al.
    Cancer Cell (2018)

    Utilizing precision genetic engineering and PHI’s HoloMonitor technology, scientists at University of California, San Francisco, have for the first time been able to monitor and map how mutations break down the genetic protection against skin cancer, allowing harmless moles to transform into invasive skin cancer.

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  • Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi
    I. Yeh et al.
    Nature Communications (2017)

    HoloMonitor was used to measure cell volume. Together with other methods the results identify DPN (deep penetrating nevus) as an intermediate melanocytic neoplasm, with a progression stage positioned between benign nevus and DPN-like melanoma.

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  • Loss of Erα Induces Amoeboid-like Migration of Breast Cancer Cells by Downregulating Vinculin
    Y. Gao et al.
    Nature Communications (2017)

    The authors tracked and analyzed the migration of different MCF-7 cell populations in a 3D matrix. In addition to measuring the cell migration speed with HoloMonitor, the following five phenotypes were observed: ‘stable adhesion’, ‘unstable lamellipodia’, ‘bleb’, ‘stable bleb’ and ‘unstable pseudopod’.

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  • Reversal of Chemoresistance in Ovarian Cancer by Co-delivery of a p-glycoprotein Inhibitor and Paclitaxel in a Liposomal Platform
    Y. Zhang et al.
    Molecular Cancer Therapeutics (2016)

    The authors created a nanoparticle formulation against chemotherapy resistant ovarian cancer, loaded with the commonly used cancer drug paclitaxel together with a drug that breaks down the resistance mechanism of cancer cells. Using HoloMonitor, the scientists were able to show that paclitaxel-resistant ovarian cancer cells stopped multiplying when treated with the nanoparticles.

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