Journal: International Immunopharmacology (2022)
Institution: Pharmaceutical College of Henan University
Research Areas: Cancer research
Cell Lines: U937, A549 (human tissue lymphoma cells, lung cancer cells)
Summary: Berberine is the active compound in Coptis Chinensis Franch, traditionally used to treat malignant sores and cancer. In vitro, Protein arginine deaminase 4 (PAD4) overexpression affects the cell-activated state in macrophages by promoting epithelial-mesenchymal transition (EMT) of A549 lung cancer cells and inhibiting cell apoptosis. The authors investigated if berberine can prevent lung cancer and explore its effect on PADI4-related macrophage function. Results suggested berberine can significantly inhibit PADI4 expression by reversing the macrophage-activated state and the lung cancer-promoting effect of PADI4-overexpressed macrophages. This finding provides a novel understanding and evidence for using berberine to prevent lung cancer. HoloMonitor M4 is used for cell morphology study for co-culturing.