Journal: FASEB BioAdvances (2022)
Institution: University of Fukui
Research Areas: Cancer research
Cell Lines: Primary fibroblasts
Summary: Xeroderma pigmentosum (XP) is a hereditary disorder characterized by a photosensitivity predisposition to skin cancers. Here, the authors investigated the role of the XP group-A (Xpa) gene in directed cell migration. Xpa-knockdown neurons in the embryonic cerebral cortex showed abnormal cell migration, cell cycle exit, and differentiation. The genotype-phenotype relationship between the lack of XPA and cell migration abnormalities was confirmed using both a scratch assay and time-lapse microscopy HoloMonitor in XP-A patient-derived fibroblasts. Unlike healthy cells, these cells showed impairment in overall mobility and the direction of motility. Therefore, abnormal cell migration may explain neural tissue abnormalities in patients with XP-A. HoloMonitor M4 was used to compare cell motility between wt and XPA knock-down fibroblasts.