Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitination
Journal: International Journal of Biological Sciences (2022)
Institution: Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China.
Research Areas: Cancer research
Cell Lines: SUM159PT, SUM149PT, MDAMB231, MCF7, BT474, SKBR3 (TNBC lines, luminal breast cancer cell lines, HER2-positive breast cancer cell line)
Summary: Cold atmospheric plasma (CAP) is selective against many cancers with few side effects, yet its molecular mechanism remains unclear. The authors propose CAP as a promoneg onco-therapy targeting cancer stemness via the AQP3/FOXO1 axis. AQP3-19Y phosphorylation was essential for SCAF11-mediated AQP3-5K K48-ubiquitination and FOXO1 stability, which was suppressed on CAP exposure. The authors also demonstrated the synergistic advantages between CAP and Atorvastatin towards enhanced anti-cancer efficacy. They advocated CAP as a promising onco-therapy, functioning alone or as an adjunct to other therapeutic modalinowards the hope of cancer eradication. HoloMonitor was used for cell migration and single cell study.