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Colony Formation, Migratory, and Differentiation Characteristics of Multipotential Stromal Cells (MSCs) from “Clinically Accessible” Human Periosteum Compared to Donor-Matched Bone Marrow MSCs

Authors: Heather E. Owston, Payal Ganguly,Giuseppe Tronci, Stephen J. Russell, Peter V. Giannoudis, Elena A. Jones

Journal: Stem Cells International (2019)

Institution: University of Leeds

Research Areas: Stem cell research

Cell Lines: Primary BM and primary MSC (Primary BM – primary human bone marrow cells Primary MSC – primary human multipotential stromal cells)

Summary: Periosteum, a highly vascular and multipotential stromal cell- (MSC-) rich tissue, is vital for fracture healing. In this study, H. E. Owston et al aimed to compare human periosteum and donor-matched iliac crest BM MSC content. They also characterized MSCs in terms of colony formation, growth kinetics, phenotype, cell migration patterns, and trilineage differentiation capacity. Authors have used HoloMonitor M4 to show that in early culture, periosteum cells were less migratory at slower speeds than BM cells. Live cell tracking allowed to determine unique morphological and migration characteristics of periosteal MSCs that can be used to develop novel bone graft substitutes.

Keywords: HoloMonitor M4, cell morphology and cell movements, multipotential stromal cell, stem cell therapy

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