Development of label-free biophysical markers in osteogenic maturation
Journal of the Mechanical Behavior of Biomedical Materials (2019)
Institution: National Research Council of Italy, Italy
Cell Line: hFOB 1.19 (Human foetal osteoblasts)
Research Area: Cell Research
Conclusions: Morphological and mechanical changes in living cells indicate functionally associated processes, such as cell maturation or death. In this study A. Bartolozzi with colleagues characterized the maturation of human fetal osteoblasts. The authors highlight that non-invasive method, such as digital holography, atomic force microscopy, and TIE, have the ability to identify effective label-free biomarkers, which help to characterize cell biomarkers required for this fundamental process. Authors have used HoloMonitor M3 to quantify cell morphology (among others, area, perimeter, and volume) changes during hFOB maturation.