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Digital Holographic Cytometry: Macrophage Uptake of Nanoprobes

Authors: Louise Sternbæk, Martha Wamaitha Kimani, Kornelia Gawlitza, Birgit Janicke, Kersti Alm, Anette Gjörloff Wingren

Journal: Imaging & Microscopy (2019)

Institution: Malmö University

Research Areas: Cytotoxicity

Cell Lines: RAW 264, 7 (Murine cell line, macrophages from blood)

Summary: Various nanoprobes are designed to have drug-delivery or detection properties. However, as a foreign bodies in human blood circulation, nanoprobes can potentially induce inflammatory response and active macrophages. In this study, L. Sternbæk et al. have employed HoloMonitor M4 to study the effects of nanoprobes (molecularly imprinted polymers targeted to sialic acid) on macrophage cell line, RAW 264.7 by evaluating cell morphology (cell area, volume, and thickness) and cell count. Authors have shown that neither the HoloMonitor M4 nor nanoprobes affected the physiological functions of macrophages.

Keywords: HoloMonitor M4, Cytotoxicity, Cell morphology, Cell proliferation

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