Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies

L. Croft et al.

Theranostics: Methods and Protocols (2021)

Institution: Queensland University of Technology, Australia

Cell Line: HeLa (Human cervical cancer cell line)

Research Area: Cancer research

Tags: HoloMonitor M4, cell proliferation, cell morphology

Conclusions: L. Croft with co-authors have prepared a protocol on how to evaluate cancer drug affects by using non-invasing digital holographic imaging, i.e. HoloMonitor M4. Authors describe the use of the DHI platform HoloMonitorM4 to evaluate the effects of novel, targeted cancer therapies on cell viability and proliferation using the HeLa cancer cell line as a model. They present single cell tracking and population-wide analysis of multiple cell morphology parameters.

Read the article …