Digital Holographic Microscopy for Non-invasive Monitoring of Cell Cycle Arrest in L929 Cells

M. Miniotis et al.

PLOS ONE (2014)

Institution: Malmö University, Sweden

Cell Line: L929 (Mouse fibroblast)

Research Area: Method development

Tags: Holomonitor M3, Cell morphology, Cell cycle and cell division, Flow cytometry, Cancer treatment, G1 phase, Colcemid treatment, Holographic microscopy, therapeutic drug

Conclusions: We show that average cell phase volume results from DHM readings are comparable to the flow cytometry findings. DHM thus provides a non-disruptive alternative to flow cytometry. The technique has the potential to develop into a fast and cost-efficient method for pre-clinical monitoring of cancer treatment efficacy.

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