Customer Publication
Dissociation of the nuclear WWOX/TRAF2 switch renders UV/cold shock-mediated nuclear bubbling cell death at low temperatures
Journal: Cell Communication and Signaling (2024)
Institution: Institute of Molecular Medicine, National Cheng Kung University, Tainan, Taiwan
Research Areas: Cell death
Cell Lines: COS7, L929S (African green monkey SV40-transfected kidney COS7 fibroblasts, mouse tumor necrosis factor (TNF)-sensitive L929S fibrosarcoma cells)
Summary: UV-treatment, alone or in combination with cold, causes two types of cell death connected to accumulation of NO gas, the bubbling cell death (BCD) or the nuclear pop-out explosion death (POD). Using a multitude of cell lines, the authors investigate the molecular pathways determining which type of cell death the cell will undergo. Using HoloMonitor M4, they demonstrated that there is an increased thickness/height in dividing or apoptotic cells. Cell volume increase occurs normally in dividing cells. The tallness lasts less than 20 min, and then the cell divides. In contrast, during apoptosis, the increased cell height can last for 5 to 8 h, followed by a breakdown of the whole cell. However, during BCD, the bubble released from the nucleus is indeed flattened, compared to the cell body, suggesting that the nuclear bubble contains NO and cytoplasmic liquid.