Customer Publication
Dissecting Morphological and Functional Dynamics of Non-Tumorigenic and Triple-Negative Breast Cancer Cell Lines Using PCA and t-SNE Analysis
Journal: Cancer Reports (2025)
Research Areas: Cancer Research
Cell Lines: MCF10A, MDA-MB-231 (triple-negative breast cancer cell lines)
Summary: Triple-negative breast cancer (TNBC) poses significant challenges due to its aggressive nature and lack of targeted therapies. Understanding the cellular behaviors of TNBC is crucial for developing effective treatments. This study aims to compare the morphological characteristics of non-tumorigenic MCF10A and aggressive MDA-MB-231 TNBC cell lines using advanced analytical techniques. The profound differences in cellular dynamics between MCF10A and MDA-MB-231 cell lines underscore the unique adaptive mechanisms of TNBC cells. Our study provides valuable insights into the cellular foundations of TNBC aggressiveness, offering a foundation for future research aimed at understanding the mechanistic underpinnings of TNBC progression and therapeutic targeting.HoloMonitor was used to study cell morphology including area, irregularity and optical thickness., as well as cell migration, motility, migration directness,