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Dual-functional injectable hydrogels as antimicrobial and angiogenic therapeutics for dental pulp regeneration†

Authors: Nhu-Quynh Thi Nguyen et al.

Journal: Journal of Materials Chemistry B (2025)

Institution: Biomaterials and Nanotechnology Research Group, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

Research Areas: Material chemistry

Cell Lines: SVEC4-10 (mouse endothelial cells)

Summary: this study investigates an injectable hydrogel system composed of hyaluronic acid (HA) and carboxymethyl chitosan (CMCs), chemically crosslinked with 1,4-butanediol diglycidyl ether (BDDE). The hydrogel demonstrated excellent injectability, self-healing ability, and mechanical stability. In vitro and in vivo assessments confirmed its biocompatibility, biodegradability, and capability for controlled release of therapeutic agents. Notably, the amoxicillin-loaded hydrogel (HACM/AX) showed significant antibacterial properties; while the erythropoietin-loaded hydrogel (HACM/EPO) significantly enhanced endothelial cell proliferation and migration, facilitating angiogenesis for tissue regeneration. These findings highlight the potential of the HACM hydrogel as a dual-function biomaterial with antimicrobial and pro-angiogenic properties, offering a promising alternative for pulpitis treatment and dental tissue engineering. HoloMonitor m4 was used to study the morphological features such as convexity, eccentricity, and cell area, cell proliferation and wound healing.

Keywords: HoloMonitor M4, cell morphology, cell proliferation, wound healing

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