Effects of pro-inflammatory cytokines and cell interactions on cell area and cytoskeleton of rheumatoid arthritis synoviocytes and immune cells
Journal: European Journal of Cell Biology (2023)
Institution: University of Lyon
Research Areas: Immunology
Cell Lines: Synoviocytes, Peripheral blood mononuclear cells (PBMCs)
Summary: Rheumatoid arthritis is a disease in which the immune system attacks the joints, leading to inflammation and the formation of pannus. In this study, the researchers investigated how inflammation affects the morphology of synoviocytes and immune cells. They found that two cytokines, IL-17 and TNF, which play a major role in RA, caused synoviocytes to retract and develop more pseudopodia. Additionally, inflammation reduced several morphological parameters, including cell confluence, area, and motility speed. The researchers also looked at how co-culturing synoviocytes and immune cells affected cell morphology, finding that inflammation or cell activation led to similar changes in both cell types. However, this effect was not seen in control synoviocytes, indicating that an inflammatory environment is necessary for these morphological changes to occur. Overall, these findings suggest that inflammation and cell interactions can dramatically alter the shape and structure of synoviocytes and immune cells in the joint, potentially leading to better interactions between cells.HoloMonitor M4 is used to study morphological changes and motility speed.