Efficient and crucial quality control of HAP1 cell ploidy status

Authors: T. Beigl et al.

Journal: Biology Open (2020)

Institution: University of Bergen

Research Areas: Cell Research

Cell Lines: HAP1 (Near haploid cell line derived from male chronic myelogenous leukemia)

Summary: Haploid cells are powerful tools to study gene functions and became a "workhorse" for CRISPR/Cas9 editing because a change/mutation in a single allele is enough to reveal a potential phenotype. In this study, T. B. Beigl with colleagues have aimed to check for fundamental differences between HAP1 cell cultures in the haploid versus diploid state. Authors have shown that it is extremely important to control the ploidy of HAP1 cells prior to phenotypic analyses and provide a streamlined procedure to obtain diploid cultures. Here HoloMonitor M4 was employed while comparing haploid and diploid HAP1 cell morphology, growth kinetics, and cell motility.

Keywords: HoloMonitor M4, Cell morphology and cell movements, HAP1, Near-haploid human cell line, CRISPR/Cas9, Gene editing, Clustered palindromic repeats, Cas9 enzyme, Cell phenotype analyses, Microscopy genetic disease cell model, Cell culture quality control

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