Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays
Journal: Scientific Reports (2020)
Institution: University of Bergen
Research Areas: Method Development
Cell Lines: NCL-H524, U2OS (NCL-H524 – human lung carcinoma cells; U2OS – human bone osteosarcoma epithelial cells)
Summary: Standard 2D cell cultures do not mimic physiological conditions, resulting in research results that are not always applicable in vivo. This paper focuses on developing cell-friendly methods where cells grow in Matrigel to create a physiologically relevant environment. Using the HoloMonitor M4, Hellesvik with colleagues have shown how to quantify cell morphology, proliferation, migration, and motility in different concentrations of Matrigel. In addition, the authors have demonstrated how to quantify the initial formation of spheroids in Matrigel and the paper introduces a novel method for measuring cell invasion.