Authors: Martin Garcia-Ryde et al.

Journal: International Journal of Molecular Sciences (2024)

Research Areas: Pulmonology

Cell Lines: Primary human lung fibroblasts and bronchoalveolar lavage cells (Human lung fibroblasts derived from lung biopsies and BAL cells derived from bronchoalveolar lavage fluid)

Summary: This study investigates differences in stress-related gene expression within lung cells from patients with chronic obstructive pulmonary disease (COPD) and healthy donors. Lung fibroblasts and bronchoalveolar lavage cells were derived, and their gene and protein expression was investigated. HoloMonitor M4 was used to study cell proliferation. They show that cigarette smoke extract (CSE) inhibited the proliferation of lung fibroblasts from both healthy and COPD subjects. Furthermore, they show that lung fibroblasts from COPD patients have an atypical ER stress gene response to CSE, which may be a contributing factor to COPD progression.

Keywords: copd, cigarette smoke extract, lung fibroblast, bronchoalveolar lavage, gene expression, er stress, protein