Externally added cystatin C reduces growth of A375 melanoma cells by increasing cell cycle time

Authors: H. Wallin et al.

Journal: FEBS Openbio (2021)

Institution: Lund University

Research Areas: Cancer research

Cell Lines: A375 (Human malignant melanoma)

Summary: Human cell growth and intracellular proteolysis is affected by naturally secreted cysteine protease inhibitors, thus it could potentially be used to suppress tumour growth. In this paper, H. Wallin with colleagues have studied the effects of externally added Cystatin C on melanoma cancer cell viability and proliferation. Authors have shown that the addition of Cystatin C and its internalization prolongs cell cycle length (division) and decreases melanoma cell proliferation and could potentially be utilized in suppressing tumor growth. HoloMonitor M4 was used to study cell proliferation, cell cycle length, and cell morphology (area and optical thickness).

Keywords: HoloMonitor M4, cell proliferation, cell morphology, cell cycle, cell growth, cysteine peptidase, digital holographic microscopy, protease inhibitor

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