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G9a-mediated repression of CDH10 in hypoxia enhances breast tumour cell motility and associates with poor survival outcome

Authors: Francesco Casciello, Fares Al-Ejeh, Mariska Miranda, Greg Kelly, Eva Baxter, Karolina Windloch, Frank Gannon, Jason S Lee

Journal: Theranostics (2020)

Institution: Berghofer Medical Research Institute

Research Areas: Cancer research

Cell Lines: MDA-MB-231, MCF-7 (MDA-MB-231: Human mammary carcinoma cell line; MCF-7 breast cancer cell line)

Summary: Epigenetic enzymes play an important role in regulating gene expression under lack of oxygen (hypoxia) in tumors. Thus during cancer metastasis, tumor hypoxia impairs cell adhesion molecule expression and allows tumor cells to detach from the primary site, migrate and establish metastases in distant areas of the body. This process is epithelial to mesenchymal transition (EMT) and leads to enhanced tumor aggressiveness, especially under hypoxia. In the present study, F. Casciello with colleagues have shown that epigenetic enzyme (G9a) accumulation in hypoxia leads to CDH10 protein transcription repression. This enhances cancer cell motility and is an integral part of hypoxia-mediated EMT in breast cancer. In this study, HoloMonitor M4 was used to precisely track single cells and quantify the total distance cells have traveled under different conditions and led authors to reach their conclusions on G9a and CDH10 role in the EMT mechanism.

Keywords: HoloMonitor M4, cell motility, G9a, hypoxia, CDH10, metastasis, breast cancer

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