Genetic deletion of HPV E7 oncogene effectively regresses HPV driven oral squamous carcinoma tumour growth
Journal: Biomedicine & Pharmacotherapy (2022)
Institution: Griffith University
Research Areas: Virus, cancer research
Cell Lines: SCC2, HEK293T cells (HPV+ OSC cell line, kidney epithelial-like cell)
Summary: The E6 and E7 are the main oncogenes that drive the carcinogenic process in the human papillomavirus (HPV)s. It is well-established that the removal of E7 dampens HPV cancer cell growth and proliferation, and this makes E7 a perfect target for HPV cancer treatment. Previous studies showed the presence of HPV E7 sensitizes both HPV-positive cervical and OSC tumors to tumor regression by inhibiting aurora kinases (AUR). In this study, authors investigated a staggering combination of aurora kinase inhibition, using AUR inhibitor, alisertib, followed by CRISPR editing of E7, to determine if this would lead to better HPV+ OSC killing. HoloMonitor M4 was used to compare cells with or without AUR inhibitor treatment.