IK Channel-Independent Effects of Clotrimazole and Senicapoc on Cancer Cells Viability and Migration
Journal: International Journal of Molecular Sciences (2023)
Institution: National Research Council
Research Areas: Cancer Research
Cell Lines: WM266-4, panc-1 (Melanoma cell line, Pancreatic cancerl cell line)
Summary: The study focuses on the role of the IK channel in the proliferation and migration of different types of cancer cells. The researchers used two different blockers, clotrimazole and senicapoc, and two cell lines: metastatic melanoma WM266-4 and pancreatic cancer Panc-1. The results showed that clotrimazole and senicapoc induced a decrease in viability and the migration of both WM266-4 and Panc-1 cells, irrespective of IK expression levels. Patch-clamp experiments on WM266-4 cells revealed Ca2±dependent, IK-like, clotrimazole- and senicapoc-sensitive currents, which could not be detected in Panc-1 cells. Neither clotrimazole nor senicapoc altered the intracellular Ca2+ concentration. These findings suggest that the effects of IK blockers on cancer cells are not strictly dependent on a robust presence of the channel in the plasma membrane. Instead, they might be due to off-target effects on other cellular targets or to the blockade of IK channels localized in intracellular organelles.HoloMonitor M4 was used to perform wound healing assay to study if clotrimazole and senicapoc affect cell migration.