Customer Publication

KLF2+ stemness maintains human mesenchymal stem cells in bone regeneration

Authors: Ying Zhou, Chao Liu, Jianxiang He, Lingqing Dong, Huiyong Zhu, Bin Zhang, Xiaoxia Feng, Wenjian Weng, Kui Cheng, Mengfei Yu, Huiming Wang

Journal: Stem Cells (2019)

Institution: Zhejiang University

Research Areas: Stem cell research

Cell Lines: hMSC, HUVEC (hMSC – human mesenchyman stem cells HUVEC – human umbilical vein endothelial cells)

Summary: Authors have studied how zinc finger protein presence influences stem cell capabilities to form blood vessels (undergo angiogenesis). In one part of this study authors have knocked down zinc finger proteins (KLF2) in mesenchymal stem cells (MSC) and in co-culture with HUVEC cells studied cell movements using HoloMonitor M4 (Figure 7C and 7D, and Video S5 and S6). Using HoloMonitor M4, Y. Zhour with colleagues, in real-time, have shown that absence of zinc finger proteins influences MSC cell motility speed and motility distance.

Keywords: HoloMonitor M4, cell movement and wound healing, angiogenesis, endothelial cell, KLF2, mesenchymal stem cell, osteogenesis, stemness, stem cell therapy

Read the article …