Label-Free Classification of Apoptosis, Ferroptosis and Necroptosis Using Digital Holographic Cytometry
Journal: Applied Sciences (2020)
Institution: University of Utah
Research Areas: Cell research
Cell Lines: 501mel (Human derived metastatic melanoma cell line)
Summary: Activation of alternative programmed cell death pathways, e.g. ferroptosis and necroptosis, is a promising therapeutic strategy for cancer cells, which evade apoptosis. However, the interplay between distinct programmed cell death pathways and cancer progression is complex and can paradoxically promote the disease. In this publication, K.L. Barker with colleagues has aimed to develop a label-free, live-cell imaging-based assay for classifying forms of programmed cell death with single-cell resolution. By employing HoloMonitor M4 and studying cell morphology parameter changes, authors have developed a method, which with high accuracy could classify cell death pathway in the test set for the melanoma cell line.