Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
Journal: Int. J. Mol. Sci. (2022)
Institution: Univ. Bordeaux
Cell Lines: SH-SY5Y, HCT116, primary neuronal cells from Sprague–Dawley rat, rat primary cortical astrocytes (human colorectal carcinoma cells)
Summary: To evaluate the impact on cell status of environmental radiofrequency signals (RF) exposure, authors expose cells to RF exposure alone or during co-exposure to RF, and chemical treatments are known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at a constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant.