Customer Publication

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Authors: Joushomme et al

Journal: Int. J. Mol. Sci. (2022)

Institution: Univ. Bordeaux

Research Areas: Enviromental science

Cell Lines: SH-SY5Y, HCT116, primary neuronal cells from Sprague–Dawley rat, rat primary cortical astrocytes (human colorectal carcinoma cells)

Summary: To evaluate the impact on cell status of environmental radiofrequency signals (RF) exposure, authors expose cells to RF exposure alone or during co-exposure to RF, and chemical treatments are known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at a constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant. HoloMonitor M4 was used to study the cell morphology changes afterRF- and sham-exposure.

Keywords: HoloMonitor M4, cell morphology, label-free techniques; radiofrequency electromagnetic fields; bioelectromagnetics; digital holographic microscopy; impedancemetry; human cell lines; rat cortex primary cells; apoptosis; autophagy

Read the article …

Phase Holographic Imaging PHI AB
Skiffervägen 48
224 78 Lund
Sweden
+46 46 38 60 80
info@phiab.com

Phase Holographic Imaging PHI Inc.
265 Franklin Street Suite 1702
Boston, MA 02110
USA
+1 617-963-5150
info@phiab.com

Invoicing

VAT: SE556542781101
faktura@phiab.com

Securities Identification

ISIN: SE0005504636
LEI: 549300JZR79QYESM6296

HoloMonitor® is a registered trademark
Copyright © 2012-2021
Phase Holographic Imaging PHI AB

Phase Holographic Image logo in white

PHI Theme 3.12.2