Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments
Journal: Int. J. Mol. Sci. (2022)
Institution: Univ. Bordeaux
Research Areas: Enviromental science
Cell Lines: SH-SY5Y, HCT116, primary neuronal cells from Sprague–Dawley rat, rat primary cortical astrocytes (human colorectal carcinoma cells)
Summary: To evaluate the impact on cell status of environmental radiofrequency signals (RF) exposure, authors expose cells to RF exposure alone or during co-exposure to RF, and chemical treatments are known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at a constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant. HoloMonitor M4 was used to study the cell morphology changes afterRF- and sham-exposure.