Low Resolution Solution Structure of Hamlet and the Importance of Its Alpha-domains in Tumoricidal Activity
PLOS ONE (2012)
Institution: Lund Unviersity, Sweden
Cell Line: A549 (Human lung carcinoma cells)
Research Area: Action of HAMLET
Conclusions: Peptides covering the alpha1 and alpha2 domains of the protein triggered rapid ion fluxes in the presence of sodium oleate and were internalized by tumor cells, causing rapid and sustained changes in cell morphology. The alpha peptide-oleate bound forms also triggered tumor cell death with comparable efficiency as HAMLET. In addition, shorter peptides corresponding to those domains are biologically active.