Mast Cell Proteases Tryptase and Chymase Induce Migratory and Morphological Alterations in Bronchial Epithelial Cells
International Journal of Molecular Sciences (2021)
Institution: Lund University, Sweden
Cell Line: BEAS-2B (BEAS-2B - Human bronchial epithelial cells)
Research Area: Cell Research
Conclusions: Mast cells (MCs) home into the epithelium in respiratory diseases, but the interaction mechanisms remain unclear. This study investigates how MC tryptase and chymase affect bronchial epithelial cells. HoloMonitor M4 was used to study cell growth and division rate, cell morphology changes, and cell motility and migration. The authors conclude that MC proteases induce cell migration and proliferative and morphological alterations in bronchial epithelial cells, suggesting that MC release of proteases may play a critical role in airway epithelial remodeling and disruption of epithelial function.