Customer Publication

Mechanical Stretch Promotes Neurite Outgrowth of Primary Cultured Dorsal Root Ganglion Neurons via Suppression of Semaphorin 3A-Neuropilin-1/Plexin-A1 Signaling

Authors: Meili Liu, Zitong An, Yu Zhang, Yuchen Xiao, Junwei Xu, Zhijun Zhao, Chongquan Huang, Anqing Wang, Gang Zhou, Ping Li, Yubo Fan

Journal: American Chemical Society (2022)

Institution: Beihang University

Research Areas: Neuronscience

Cell Lines: DRG neurons

Summary: Significant attempts have been made to promote neuronal extension and migration in nerve development and regeneration. This study investigated the effects of mechanical stretch on dorsal root ganglion neuronal growth and the underlying mechanisms by assessing the extension direction, neurite length, cell body size, mitochondrial membrane potential, and the expression of Sema3A and its receptors. The results showed that cell viability significantly increased at 2.5, 5, and 10% tensile strains for 4 h. Moreover, neurons migrated closer to the stretching direction at 5% tensile strain (0–12 h), while the control group moved disorderly. Furthermore, the Sema3A-Neuropilin-1/Plexin-A1 signaling pathway was found to be suppressed after a mechanical stretch at 5% tensile strain for 4 h. Finally. A Sema3A-SiRNA treatment led to remarkable guidance growth in the stretch-grown neurons. This study provides a promising new approach for developing mechanical stretching therapy or guidance factor-related drugs in injured neuronal regeneration. HoloMonitor M4 was used in this study to study the axonal trajectory to evaluate neuronal motility and migration.

Keywords: HoloMonitor M4, cell motility and migration, elastic silica membranes, stretch, semaphorin3A, Neuropilin-1, Plexin-A1, axonal growth, nerve regeneration

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