Non-invasive, quantitative assessment of the morphology of γ-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy

T. Kawase et al.

International journal of radiation biology (2016)

Institution: Niigata University, Japan

Cell Line: MSC-AT, MSC-BM (Mesenchymal stem cells derived from adipose tissue (MSC-AT) and bone marrow (MSC-BM))

Research Area: Stem cell therapy

Tags: Holomonitor M4, Cell morphology, Cell volume, Holographic microscopy, Mesenchymal stem cells, Periosteal cells, γ-ray irradiation

Conclusions: To assure the quality of cells to be used in cell therapy, we examined the applicability of digital holographic microscopy (DHM) for non-invasive, quantitative assessment of changes in cell morphology. Cell morphological parameters (e.g. cell volume) monitored by HoloMonitor could be useful and more stable than the DNA damage markers currently used.