Customer Publication

Oral bacteria accelerate pancreatic cancer development in mice

Authors: Elias Saba et al.

Journal: Gut (2024)

Institution: Institute of Biomedical and Oral Research

Research Areas: Cancer Research

Cell Lines: Panc-1, MIA-PaCa- 2, AsPC-1, BxPC-3, Panc02 (Human pancreatic ductal adenocarcinoma cell lines)

Summary: Epidemiological studies highlight an association between pancreatic ductal adenocarcinoma (PDAC) and oral carriage of the anaerobic bacterium Porphyromonas gingivalis. P. gingivalis migrated from the oral cavity to the pancreas in mice and can be detected in human PanIN lesions. Repetitive P. gingivalis administration to wild-type mice induced pancreatic acinar-to-ductal metaplasia (ADM), and altered the composition of the intrapancreatic microbiome. In iKC mice, P. gingivalis accelerated PanIN to PDAC progression. In vitro, P. gingivalis infection induced acinar cell ADM markers SOX9 and CK19, and intracellular bacteria protected PDAC cells from reactive oxygen species-mediated cell death resulting from nutrient stress. Taken together, the findings demonstrate a causal role for P. gingivalis in pancreatic cancer development in mice. HoloMonitor M4 used to count the cells.

Keywords: HoloMonitor M4, cell count, pancreatic cancer

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