Pigment Epithelium Derived Factor Is Involved in the Late Phase of Osteosarcoma Metastasis by Increasing Extravasation and Cell-Cell Adhesion
Journal: Frontiers in Oncology (2022)
Institution: Department of Molecular Medicine and Biochemistry, Graduate School and Faculty of Medicine, Akita University, Japan
Research Areas: Cancer Research
Cell Lines: 143B, TKD2 cell (osteosarcoma cell, mouse kidney endothelial cell)
Summary: To investigate the factors involved in organ #tropism in metastasis, SerpinF1/Pigment epithelium-derived factor (PEDF) was identified as the primary target for #kidneymetastasis. Overexpression of PEDF metastasized to the lungs and kidneys by triggering mesenchymal-to-epithelial transition (MET). Moreover, PEDF has shown anti-metastatic effects in epithelial cancer cells by hindering wound healing activity. Furthermore, novel interactions were observed among PEDF, lamR, lysyl oxidase-like 1 (Loxl1), and SNAI3 (Snail-like transcription factor) during the endothelial-to-mesenchymal transition (EndoMT). The results show that PEDF induces cancer #cellextravasation by increasing the permeability of kidney and lung vasculature acting via lamR and its downstream genes. HoloMonitor M4 was used for cell proliferation and wound healing studies.