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Preparation of fibrin hydrogels to promote the recruitment of anti-inflammatory macrophages

Authors: Ryusuke Tanaka, Yoichi Saito, Yukio Fujiwara, Jun-ichiro Jo, Yasuhiko Tabata

Journal: Acta Biomaterialia (2019)

Institution: Kyoto University

Research Areas: Inflammation

Cell Lines: Monocytes/Macrophages (Mouse bone marrow-derived monocytes/macrophages, human monocyte-derived macrophages (HMDMs))

Summary: During tissue regeneration macrophages, especially M2 type, play critical role in a successful healing process. Polymerized fibrin, fibrinogen, acts as a scaffold, into which macrophages can infiltrate to heal the injury. In this paper R. Tanaka et al. have fabricated fibrin hydrogel scaffolds, which promote anti-inflammatory polarization of both mouse and human macrophages. Authors have shown that fibrin hydrogel alone exerts adequate promoting effect on macrophage recruitment (M2 type) when compared to a fibrin hydrogel loaded with sphingosine-1-phosphate receptor 1 selective agonist, SEW2871. In this study, HoloMonitor M4 was used to study the ability of human-monocyte-derived macrophage to degrade fibrin hydrogel. Authors have shown that most of the macrophages did not adhere to the surface, and the decomposition of the fibrin hydrogel was performed by many nonadherent aggregates in cooperation with a small number of adherent cells.

Keywords: HoloMonitor M4, Cell morphology, Macrophages, Fibrin, Anti-inflammation, Controlled release, Tissue engineering, Tissue regeneration

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